Absence of Mesothelial Cells from Tuberculous Pleural Effusions

Spriggs, A. I.; Boddington, Michael M.

doi:10.1136/thx.15.2.169

Cited by 56 publications

(13 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This might have been due to the lack of mesothelial cells in these effusions. Mesothelial cells are usually absent in tuberculous effusions; indeed, the absence of mesothelial cells, together with the presence of increased numbers of lymphocytes in pleural effu- sions, is currently considered a diagnostic criterion for TB (28,29). The high concentrations of soluble IL-8 in these effusions might be due to production of this cytokine by mesothelial cells that remain in the pleural lining and are not shed into the effusion.…”

Section: Discussionmentioning

confidence: 97%

Interleukin-8 Induces Lymphocyte Chemotaxis into the Pleural Space

Pace

Gjomarkaj

Melis

et al. 1999

Am J Respir Crit Care Med

View full text Add to dashboard Cite

The pleural space is a potential compartment between the lung and chest wall that becomes filled with fluid and inflammatory cells in a number of respiratory diseases. In an attempt to understand one aspect of the inflammatory process in the pleural space, we compared the responses in three different diseases (congestive heart failure [CHF], tuberculosis [TB], and cancer). Large concentrations of interleukin-8 (IL-8) were detected in cancer and TB effusions, but not in CHF. Surprisingly, the concentration of IL-8 correlated best with lymphocyte recruitment and not with neutrophil recruitment. Pleural fluid from cancer and TB patients was chemotactic for lymphocytes, and this activity was partly blocked by an anti-IL-8 antibody in cancer and completely blocked in TB. To determine whether there was the potential for a chemotactic gradient into the pleural space, pleural effusion cells were analyzed for the expression of IL-8. Cells in the effusions of cancer patients expressed IL-8, whereas IL-8 could not be detected from the cells of TB and CHF effusions. To explore the possible role of pleural macrophages in the regulation of IL-8, pleural effusion cells were treated with culture supernatants from stimulated pleural macrophages. Stimulated pleural macrophages were able to induce expression of messenger RNA (mRNA) for IL-8 and IL-8 protein production, and this activity was abrogated by blocking tumor necrosis factor-alpha. These findings suggest that soluble IL-8 is an important factor for the recruitment of lymphocytes into the pleural space, and that this cytokine is produced by both pleural structural and cancer cells after their activation by macrophage-derived, cytokine-mediated signals.

show abstract

Section: Discussionmentioning

confidence: 97%

Interleukin-8 Induces Lymphocyte Chemotaxis into the Pleural Space

Pace

Gjomarkaj

Melis

et al. 1999

Am J Respir Crit Care Med

View full text Add to dashboard Cite

show abstract

“…On the other hand, lymphocytic fluid suggests either cancer or tuberculosis (Yam, 1967;Light et al, 1973), although the absence or scarcity of mesothelial cells is a more constant characteristic than lymphocytosis in tuberculosis (Spriggs and Boddington, 1960). We confirmed the following characteristics: in tuberculous effusion the fluid contained the greatest number of cells, was the most lymphocytic, and had only a few mesothelial cells; whereas in cancerous effusion the fluid contained the smallest number of cells, was rarely lymphocytic, and contained the greatest number of mesothelial cells.…”

Section: Discussionmentioning

confidence: 99%

Pleural effusion: laboratory tests in 300 cases.

Hirsch¹,

Ruffié²,

Nebut³

et al. 1979

Thorax

192

View full text Add to dashboard Cite

The cause of pleural effusion was studied in 300 consecutive patients by clinical examination and laboratory tests. The three most common causes were found to be cancer 117 cases (metastatic 65, bronchogenic Pleural effusion represents a very common diagnostic problem. In all studies on the causes of this condition, apart from those that are evident, such as congestive heart failure and cirrhosis, the percentage of undetermined causes is still around 20%, even after complete diagnostic evaluation.We determined the origin of pleural effusion in patients with subacute or chronic conditions admitted to a department of thoracic diseases. The purpose of this study was to determine the specific cause-malignancy or tuberculous or bacterial infection-and ResultsThe most frequent causes of pleural effusion were cancer (39%), tuberculous infection (17 6%), and bacterial infection (126%): 20f6% were undiagnosed, and other causes represented 10% (table 1).

show abstract

“…Unfortunately, none of the above features are specific for tuberculosis and similar pleural fluid characteristics are seen in parapneumonic effusions, some malignancies and rheumatoid disease involving the pleura and additional tests are required in most cases. The presence of significant mesothelial cells or in the pleural fluid argues against tuberculous pleurisy and should prompt the investigating clinician to consider an alternative diagnosis [20].…”

Section: Biochemical and Cellular Analysis Of Pleural Fluidmentioning

confidence: 99%

Pleural tuberculosis

Chakrabarti

Davies

2016

Monaldi Arch Chest Dis

View full text Add to dashboard Cite

Pleural effusions in tuberculosis are commonly seen in young adults as an immunological phenomenon occurring soon after primary infection. However, the epidemiology and demographics of tuberculous pleurisy are changing due to the impact of HIV co-infection and the increasing number of pleural effusions seen as part of re-activation disease. Pleural biopsy for histology and culture is the mainstay of diagnosis with closed needle biopsy adequate in the majority of cases. Techniques such as PCR of biopsy specimens and the role of pleural fluid ADA are still being evaluated as a diagnostic aid. Tuberculous empyema is less commonly seen in the western world and the diagnostic yield from pleural fluid here is greater than in “primary” effusions. Treatment with appropriate antituberculous chemotherapy is generally successful though there is currently insufficient evidence to recommend the routine use of corticosteroids in this condition.

show abstract

Absence of Mesothelial Cells from Tuberculous Pleural Effusions

Cited by 56 publications

References 1 publication

Interleukin-8 Induces Lymphocyte Chemotaxis into the Pleural Space

Interleukin-8 Induces Lymphocyte Chemotaxis into the Pleural Space

Pleural effusion: laboratory tests in 300 cases.

Pleural tuberculosis

Contact Info

Product

Resources

About