Absence of Leishmaniinae and Nosematidae in stingless bees

Nunes‐Silva, Patrícia; Piot, Niels; Meeus, Ivan; Blochtein, Betina; Smagghe, Guy

doi:10.1038/srep32547

Cited by 23 publications

(9 citation statements)

References 31 publications

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“…Little is known about pathogens in stingless bees; however, there are no pathogen transfer record from A. mellifera [113], which shows the lack of information about microorganisms in Meliponini.…”

Section: Microorganisms In Stingless Bee Honeymentioning

confidence: 99%

Microorganisms in Honey

Silva¹,

Rabadzhiev²,

Eller³

et al. 2017

Honey Analysis

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Honey is a product with low water activity because of the great amount of sugars (fructose and glucose), and also it has antimicrobial compounds derived from flowers or because of its transformation process in the beehive. Despite all the honey microorganism barriers, some species of microorganisms are able to survive and may cause damage to honeybees or consumers. Techniques of pathogenic microorganism identification by DNA using PCR are recommended and required for sanitary and customs control. It is important to know the diversity of contaminating microorganisms in honey, especially due to disseminate pathogenic microorganisms in the international traded marketing. In contrast, beneficial microorganisms such as yeasts can remain latently in this product waiting for the moment in which the environment is suitable for their development. Among the beneficial bacteria found in honeybee products, we can mention some lactic acid bacteria that act as prebiotics when ingested. The microorganisms in the digestive tract of honeybees are important for their health. Thus, we present the knowledge of microbiota associated with honey from honeybees and stingless bees (Hymenoptera, Apidae) and the techniques available for the detection of microorganisms in honey.

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Section: Microorganisms In Stingless Bee Honeymentioning

confidence: 99%

Microorganisms in Honey

Silva¹,

Rabadzhiev²,

Eller³

et al. 2017

Honey Analysis

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“…bombi , or (more recently) N . bombi could have been introduced by alien bees and then transferred to native ones (Arbetman et al 2013;Plischuk et al 2017); however, temporarily space incompatible findings have suggested some weaknesses in this hypothesis (Gamboa et al 2015;Nunes-Silva et al 2016). Throughout our contribution, we suggest that these three species were in fact present in the region long before the introduction of B .…”

Section: Discussionmentioning

confidence: 67%

Phoretic mites on South American bumblebees (Bombus spp.) as parasite carriers: a historical input

et al. 2020

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Phoretic mites associated with bumblebees are considered commensals that represent little or no risk for their hosts. The aim of the present study was to assess the potential role of the phoretic mites Pneumolaelaps longanalis and Parasitellus fucorum , as carriers of parasites that are known to affect bees. Mites were extracted from bumblebees dating between 1945 and 1981 which belong to museum collections, and from a freshly collected queen. The DNA was extracted and amplified, and the final products were sequenced and analyzed. Parasite's DNA was found in all samples. Ascosphaera spp., Crithidia bombi , Lotmaria passim , and Nosema ceranae were present in both mite species. Moreover, DNA from Apis mellifera filamentous virus (AmFV), Apicystis bombi , Ascosphaera apis , Nosema apis , and Nosema bombi was found in P. longanalis. This study represents the oldest report of parasite's DNA in bumblebee phoretic mites, highlighting the relevance of museum samples for historical studies in parasitology.

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“…Once the species were identified, total genomic DNA was individually extracted using a High Pure PCR Template Preparation kit (Roche Diagnostics). DNA amplification of a 134 bp PCR product (5'-AGATGGGGGCATTCGTATTG-3', 5'-ATCTGATCGCCTTCGAACCT-3') was performed as internal control (Nunes-Silva et al, 2016). AmFV DNA was detected using primers which amplify a 551 bp fragment of the Bro-N gene of AmFV (Gauthier et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

Broad Geographic and Host Distribution of Apis mellifera Filamentous Virus in South American Native Bees

Quintana

Landa

Revainera

et al. 2019

Journal of Apicultural Science

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Apis mellifera filamentous virus (AmFV) is a large double stranded DNA virus of honey bees and its prevalence and relationship with other parasites is poorly known. Samples consisted of fifty-one adult bees belonging to eight native species collected using entomological nets in six provinces of Argentina, from 2009 to 2018. Total genomic DNA was extracted from individual bees and a 551 bp fragment of the Bro-N gene of AmFV was amplified by qPCR. In the present work we have reported for the first time both the presence and the wide geographic distribution of AmFV in Argentinian species of native bees. This is the first report of the presence of this virus associated with Xylocopa atamisquensis, X. augusti, X. frontalis, X. spendidula, Bombus pauloensis and Peponapis fervens. Detecting pathogens that could threaten native bee health is of outmost importance to generate both conservation and management strategies.

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Absence of Leishmaniinae and Nosematidae in stingless bees

Cited by 23 publications

References 31 publications

Microorganisms in Honey

Microorganisms in Honey

Phoretic mites on South American bumblebees (Bombus spp.) as parasite carriers: a historical input

Broad Geographic and Host Distribution of Apis mellifera Filamentous Virus in South American Native Bees

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