Absence of jaagsiekte sheep retrovirus DNA and RNA in bronchioloalveolar and conventional human pulmonary adenocarcinoma by PCR and RT-PCR analysis

Yousem, Samuel A.; Finkelstein, Sydney; Swalsky, Patricia A.; Bakker, Anke; Ohori, N. Paul

doi:10.1053/hupa.2001.28249

Cited by 40 publications

(25 citation statements)

References 16 publications

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“…However, these results have not been confirmed by further molecular studies and 2 PCR-based analyses failed to detect any exogenous or endogenous JSRV-related genome in human BAC and adenocarcinoma [35,92].…”

Section: Jsrv and Human Lung Cancermentioning

confidence: 54%

Jaagsiekte Sheep Retrovirus (JSRV): from virus to lung cancer in sheep

et al. 2007

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-Jaagsiekte Sheep Retrovirus (JSRV) is a betaretrovirus infecting sheep. This virus is responsible for a pulmonary adenocarcinoma, by transformation of epithelial cells from the bronchioli and alveoli. This animal cancer is similar to human bronchioloalveolar cancer (BAC), a specific form of human lung cancer for which a viral aetiology has not yet been identified. JSRV interacts with target cells through the membrane receptor Hyal2. The JSRV genome is simple and contains no recognised oncogene. It is now well established that the viral envelope protein is oncogenic by itself, via the cytoplasmic domain of the transmembrane glycoprotein and some domains of the surface glycoprotein. Activation of the PI3K/Akt and MAPK pathways participates in the envelopeinduced transformation. Tumour development is associated with telomerase activation. This review will focus on the induction of cancer by JSRV.

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Section: Jsrv and Human Lung Cancermentioning

confidence: 54%

Jaagsiekte Sheep Retrovirus (JSRV): from virus to lung cancer in sheep

et al. 2007

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“…However, there is no clear consensus on the correlation between JSRV and human lung cancer (Yousem et al, 2001;De Las Heras et al, 2007;Hopwood et al, 2010). Interestingly, one study has reported that a subset of human lung cancers expressed an antigen that could react with a JSRV Envspecific monoclonal antibody.…”

Section: Discussionmentioning

confidence: 99%

Detection of Jaagsiekte sheep retrovirus in the peripheral blood during the pre-clinical period of ovine pulmonary adenomatosis

Liu¹,

Zhang²,

Sun³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The envelope protein (Env) of the Jaagsiekte sheep retrovirus (JSRV) is known to be a unique oncoprotein responsible for inducing ovine pulmonary adenocarcinoma (OPA). The objective of this study was to prepare a specific monoclonal antibody (mAb) against the JSRV Env protein using bioinformatic analysis. According to the structure and epitope prediction results of JSRV Env, the JSRV-Env 572-615 antigen was prepared via peptide synthesis (amino acid sequence 572-615, denoted as JSRV-Env 572-615 ). BALB/c mice were immunized to prepare the anti-JSRV-Env 572-615 mAb. Spleen cells were fused with SP2/0 myeloma cells after being screened by indirect ELISA and cloned by limiting dilution. The specificity of mAb was evaluated by western blot analysis and immunohistochemistry assays. Western blot results showed that the JSRV Env protein was able to bind to mAb with high specificity. Immunohistochemistry assays demonstrated that the mAb was able to recognize JSRV Env in adenomatous hyperplasia of the lung. Furthermore, JSRV was detected in peripheral blood leukocytes during the pre-clinical period of OPA in 2 of the 25 sheep using this newly synthesized mAb. Therefore, this mAb may be a useful tool for the detection of JSRV in sheep.

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“…In one case, orf-X-like and gag-pro-like sequences were detected in 10-26% of lung cancer patients and healthy individuals in Nigeria and Cameroon, but were not present in samples from France, Germany and Russia (Morozov et al, 2004). In two other studies, JSRV-like sequences were not detected (Yousem et al, 2001;Hiatt and Highsmith, 2002). Together these studies suggest the possibility of JSRV involvement in human lung cancers, but more work is needed to resolve this issue.…”

Section: Sheep Retroviral Oncogenesis: a Historical Perspectivementioning

confidence: 82%

Oncogenic transformation by the jaagsiekte sheep retrovirus envelope protein

Liu

Miller

2006

Oncogene

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Retroviruses have played profound roles in our understanding of the genetic and molecular basis of cancer. Jaagsiekte sheep retrovirus (JSRV) is a simple retrovirus that causes contagious lung tumors in sheep, known as ovine pulmonary adenocarcinoma (OPA). Intriguingly, OPA resembles pulmonary adenocarcinoma in humans, and may provide a model for this frequent human cancer. Distinct from the classical mechanisms of retroviral oncogenesis by insertional activation of or virus capture of host oncogenes, the native envelope (Env) structural protein of JSRV is itself the active oncogene. A major pathway for Env transformation involves interaction of the Env cytoplasmic tail with as yet unidentified cellular adaptor(s), leading to the activation of PI3K/Akt and MAPK signaling cascades. Another potential mechanism involves the cell-entry receptor for JSRV, Hyaluronidase 2 (Hyal2), and the RON receptor tyrosine kinase, but the exact roles of these proteins in JSRV Env transformation remain to be better understood. Recently, a mouse model of lung cancer induced by JSRV Env has been developed, and the tumors in mice resemble those seen in sheep infected with JSRV and in humans. In this review, we summarize recent progress in our understanding the molecular mechanisms of oncogenic transformation by JSRV Env protein, and discuss the relevance to human lung cancer.

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Absence of jaagsiekte sheep retrovirus DNA and RNA in bronchioloalveolar and conventional human pulmonary adenocarcinoma by PCR and RT-PCR analysis

Cited by 40 publications

References 16 publications

Jaagsiekte Sheep Retrovirus (JSRV): from virus to lung cancer in sheep

Jaagsiekte Sheep Retrovirus (JSRV): from virus to lung cancer in sheep

Detection of Jaagsiekte sheep retrovirus in the peripheral blood during the pre-clinical period of ovine pulmonary adenomatosis

Oncogenic transformation by the jaagsiekte sheep retrovirus envelope protein

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