Absence of human T‐lymphotropic virus type I <i>tax</i> sequences in a population of normal blood donors in the Baltimore, MD/Washington, DC, area: results from a multicenter study

Cowan, Elliot P.; Nemo, George J.; Williams, Alan E.; Alexander, Rosemary K.; Vallejo, Alejandro; Hewlett, Indira; Lal, R. B.; Dezzutti, Charlene S.; Gallahan, Dan; George, Kirsten St.; Pancake, Bette A.; Zucker‐Franklin, Dorothea; McCurdy, Paul R.; Tabor, Edward

doi:10.1046/j.1537-2995.1999.39080904.x

Cited by 13 publications

(4 citation statements)

References 16 publications

(32 reference statements)

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“…Given that the UL123 assay (Lab 3) demonstrated the best analytical sensitivity of the assays examined, we believe that further investigations are merited to determine the reasons for the positive PCR results with the S–/WB– samples. Potential approaches include amplifying and sequencing the UL123 amplicon as well as CMV targets with known sequence variations (UL74, UL146, UL144) to determine if contamination occurred and conducting an additional multicenter analysis of this assay method, as was recently undertaken to resolve discrepant results of antibody and DNA screening for HTLV‐I 32,33 …”

Section: Discussionmentioning

confidence: 99%

Multicenter evaluation of PCR methods fordetecting CMV DNA in blood donors

et al. 2001

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Seven CMV PCR assays currently used for research and/or diagnostic applications displayed marked variations in sensitivity, specificity, and reproducibility when applied to coded analytical and clinical control samples containing cellular DNA from the equivalent of 250,000 WBCs. These results will be useful in the selection of assays with performance characteristics appropriate to donor screening objectives. They may also help explain discrepant findings from previous studies that used PCR to determine CMV DNA prevalence in seronegative and seropositive blood donors.

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Section: Discussionmentioning

confidence: 99%

Multicenter evaluation of PCR methods fordetecting CMV DNA in blood donors

et al. 2001

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“…HTLV‐1 defective genomes were found to be present in peripheral blood mononucleal cells and cutaneous lesions of patients with MF 29,30 . However, several groups failed to detect tax‐like sequences in patients with MF from USA 31,32 . Moreover, recent molecular biological studies of a diffuse population of patients with cutaneous T‐cell lymphoma (CTCL) failed to detect HTLV‐1 genetic sequences in lesional DNA 33,34 .…”

Section: Atllmentioning

confidence: 99%

Human T‐lymphotropic Virus 1 (HTLV‐1) infection – dermatological implications

Amano¹,

Setoyama²,

Grant³

et al. 2011

Int J Dermatology

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Human T-lymphotropic virus type 1 (HTLV-1) is a type C retrovirus primarily endemic to Japan, Central and South America, the Middle East, regions of Africa, and the Caribbean. Currently, an estimated 10-20 million people worldwide are infected with this virus. Although the majority of infected individuals remain asymptomatic, HTLV-1 is the causative agent of a number of disorders, notably adult T-cell leukemia/lymphoma (ATLL) and a progressive demyelinating neurological disorder, HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). In addition to ATLL and HAM/TSP, HTLV-1 has been associated with a spectrum of skin disorders, such as infective dermatitis associated with HTLV-1, crusted scabies, and leprosy. The understanding of the interaction between virus and host response has improved markedly, but there are still few treatment options.

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“…The implication from these findings, that healthy blood donors may harbour a defective virus containing only HTLV Tax sequences, and therefore escape detection by standard serological assays for HTLV‐I and ‐II, is of concern. Subsequently, a separate study of 293 healthy blood donors from New York City found no ‘Tax’ only positive donations [14], which has been supported by a multicentre study [15]. Since there may be a demographic determinant influencing the results we carried out our own pilot survey of Scottish blood donors testing plasma and lymphocytes for the presence of HTLV‐I Tax sequences.…”

Section: Discussionmentioning

confidence: 80%