Absence of human papillomavirus sequences in epithelial breast cancer in a Mexican female population

Herrera-Romano, Lisbeth; Fernández-Tamayo, Nora; Gómez-Conde, Eduardo; Reyes-Cardoso, Juan M.; Ortiz-Gutierrez, Felipe; Ceballos, Guillermo; Valdivia, Alejandra; Piña, Patricia; Salcedo, Mauricio

doi:10.1007/s12032-011-0059-x

Cited by 17 publications

(11 citation statements)

References 29 publications

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“…Therefore, our results, which can represent the basis for future studies, should be carefully evaluated because only a few HPV positive cases were identified and other studies found HPV in breast tissues with estrogen-receptor expression [ 24 ]. Moreover, the low viral load, detected in several studies [ 25 , 26 ], can explain the low frequency of HPV-positive series.…”

Section: Resultsmentioning

confidence: 99%

HPV infection and triple-negative breast cancers: an Italian case-control study

Piana

Sotgiu

Muroni

et al. 2014

Virol J

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BackgroundBreast cancer is one of the most important neoplasia among women. To reduce its incidence and mortality impact it would be desirable to early identify risk factors associated with its development. It was recently suggested that biological agents could be the etiological cause, particularly Human Papilloma Virus (HPV). No specific relationship with different breast cancer types has been demonstrated until now. In particular, the triple-negative breast cancer (TNBC), characterized by a receptor negative pattern (ER/PgR/HER2–negative) and poor prognosis, can represent one of the most relevant clinical and public health priority in terms of observational research.FindingsAim of the study was to evaluate the HPV-positivity prevalence in two breast cancer series (TNBC vs. non-TNBC) in Northern Sardinia, Italy. The sample size of each group was represented by 40 formalin-fixed and paraffin-embedded specimens. The mean age was 60.3 years. The majority of the cancers were ductal (84%). The grading distribution was different: G2 was the most prevalent grade in the non-TNBC series, whereas G3 was the most frequent in the TNBC series (70% and 72%, respectively). Six biological samples were HPV-positive (7.5%): the positivity was assessed only in the TNBC group (15%; p-value: 0.026). The isolated genotypes were: 16, 31, 45, 52, 6, and 66. Only one co-infection was found (i.e., HPV-6 and -66).ConclusionsThe prevalence of HPV-positivity in TNBC specimens was 15%. On the basis of its carcinogenetic ability, an etiological role in the pathogenesis of the cancer could be supposed. This association should be confirmed with longitudinal studies to better assess the role of the HPV infection in TNBC and non-TNBC tumors.

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Section: Resultsmentioning

confidence: 99%

HPV infection and triple-negative breast cancers: an Italian case-control study

Piana

Sotgiu

Muroni

et al. 2014

Virol J

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“…Most of the published studies do not follow a precise methodology, and the screening criteria used are very heterogeneous. Some studies only consider juvenile malignancies [34], while others include inflammatory breast cancer tissues [48, 49], triple-negative tumors [50], medullary malignancies [51], metaplastic breast cancer [52], papillary lesions [20, 53], Paget’s disease [54], or carcinoma in situ [9, 24, 25, 36, 41, 55–60]. In addition, no standards are used in selecting the molecular technique to screen for viruses, implying the potential detection of different viral serotypes.…”

Section: Discussionmentioning

confidence: 99%

Presence of human papillomavirus DNA in breast cancer: a Spanish case-control study

Delgado-García

Martínez‐Escoriza

Alba³

et al. 2017

BMC Cancer

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BackgroundBreast cancer is one of the most important neoplasia among women. It was recently suggested that biological agents could be the etiological cause, particularly Human Papilloma Virus (HPV). The aim of this study was to explore the presence of HPV DNA in a case-control study.MethodsWe performed our study including 251 cases (breast cancer) and 186 controls (benign breast tumors), using three different molecular techniques with PCR (GP5/GP6, CLART® and DIRECT FLOW CHIP®).ResultsHPV DNA was evidenced in 51.8% of the cases and in 26.3% of the controls (p < 0.001). HPV-16 was the most prevalent serotype. The odds ratio (OR) of HPV within a multivariate model, taking into account age and breastfeeding, was 4.034.ConclusionsOur study, with methodological rigour and a sample size not previously found in the literature, demonstrate a significant presence of HPV DNA in breast cancer samples. A possible causal relationship, or mediation or not as a cofactor, remains to be established by future studies.

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“…While these data indicate that HPVs may have a possible role in some breast cancers, they may not have a major causal role. There are two reasons for this view: (i) in immunocompromised patients (due to either human immunodeficiency infections or post organ transplantation therapy), there is no increased prevalence of breast cancer, which is in contrast to the two- to sixfold increased prevalence of HPV associated cervical and head and neck cancer in these patients ( 10 ), and (ii) the HPV viral load in breast cancer is extremely low ( 11 , 12 ).…”

Section: Introductionmentioning

confidence: 99%

Human Papilloma Viruses and Breast Cancer

et al. 2015

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PurposeHuman papillomaviruses (HPV) may have a role in some breast cancers. The purpose of this study is to fill important gaps in the evidence. These gaps are: (i) confirmation of the presence of high risk for cancer HPVs in breast cancers, (ii) evidence of HPV infections in benign breast tissues prior to the development of HPV-positive breast cancer in the same patients, (iii) evidence that HPVs are biologically active and not harmless passengers in breast cancer.MethodsRNA-seq data from The Cancer Genome Atlas (TCGA) was used to identify HPV RNA sequences in breast cancers. We also conducted a retrospective cohort study based on polymerase chain reaction (PCR) analyses to identify HPVs in archival specimens from Australian women with benign breast biopsies who later developed breast cancer. To assess whether HPVs in breast cancer were biologically active, the expression of the oncogenic protein HPV E7 was assessed by immunohistochemistry (IHC).ResultsThirty (3.5%) low-risk and 20 (2.3%) high-risk HPV types were identified in 855 breast cancers from the TCGA database. The high risk types were HPV 18 (48%), HPV 113 (24%), HPV 16 (10%), HPV 52 (10%). Data from the PCR cohort study indicated that HPV type 18 was the most common type identified in breast cancer specimens (55% of 40 breast cancer specimens) followed by HPV 16 (13%). The same HPV type was identified in both the benign and subsequent breast cancer in 15 patients. HPV E7 proteins were identified in 72% of benign breast specimens and 59% of invasive breast cancer specimens.ConclusionThere were four observations of particular interest: (i) confirmation by both NGS and PCR of the presence of high-risk HPV gene sequences in breast cancers, (ii) a correlation between high-risk HPV in benign breast specimens and subsequent HPV-positive breast cancer in the same patient, (iii) HPVs in breast cancer are likely to be biologically active (as shown by transcription of HPV DNA to RNA plus the expression of HPV E7 proteins), (iv) HPV oncogenic influences may occur early in the development of breast cancer.

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Absence of human papillomavirus sequences in epithelial breast cancer in a Mexican female population

Cited by 17 publications

References 29 publications

HPV infection and triple-negative breast cancers: an Italian case-control study

HPV infection and triple-negative breast cancers: an Italian case-control study

Presence of human papillomavirus DNA in breast cancer: a Spanish case-control study

Human Papilloma Viruses and Breast Cancer

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