Absence of high-affinity binding sites for prolactin on the bovine corpus luteum

Bevers, M.M.; Russcher, J.A.; Dieleman, S.J.

doi:10.1016/0378-4320(89)90010-9

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…The receptors possessed affinities for the ligand similar to those found in other cell systems [36,41]. However, the number of specific binding sites was smaller than the number found by Suh et al [36], who immortalized primary granulosa cells by the SV40 large T antigen, Ras oncogene, and LH-containing plasmid.…”

Section: Discussionmentioning

confidence: 76%

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Secretion of Steroids, Growth Factors, and Cytokines by Immortalized Mouse Granulosa Cell Lines1

Vanderstichele

Delaey

Winter

et al. 1994

Biology of Reproduction

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The expression and function of gonadotropin receptors, and the secretion of steroids, transferrin, and cytokines were investigated in three immortalized (single transfection with v-myc) mouse granulosa cell lines (GRM01, GRM01L, and GRM02). A dose-dependent increase in progesterone production was obtained in GRM01 and GRM02 cells after addition of LH, FSH, modulators of the adenylate cyclase enzyme system, and cAMP analogues. The LH-induced release of progesterone was already detectable in GRM02 cells after 8 h and was related to incubation time and cell number. Both epidermal growth factor (EGF) and transforming growth factor alpha (TGF alpha) induced the secretion of progesterone in GRM02 cells, while no effect was obtained with TGF beta. LH receptor concentration was highest in the GRM02 cell line. FSH receptor mRNA was visualized in GRM01 and GRM02 cells. Aromatase activity in GRM02 cells was induced by androgens and inhibited by aromatase inhibitors. Whereas all cell lines were able to secrete transferrin, only in GRM01 cells was transferrin secretion increased significantly by LH. FSH did not affect transferrin secretion in the three cell lines, in contrast to forskolin or 8-bromo-cAMP. The immortalized mouse granulosa cell lines were able to express and release several growth factors. The expression and secretion of activin, inhibin, TGF beta, EGF, TGF alpha, insulin-like growth factor II, fibroblast growth factor (acidic and basic), platelet-derived growth factor, and interleukin-6 suggest an autocrine or paracrine role for these factors in follicular differentiation and function. In conclusion, these cells, derived from mural granulosa cells and immortalized in a preovulatory state, can be used to study granulosa cell physiology or to study the role of granulosa cells and their derivatives in the process of follicular maturation, fertilization, and early embryonic development.

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Section: Discussionmentioning

confidence: 76%

“…The expression of LH/hCG receptors in the immortalized mouse granulosa cell lines was measured according to Bevers et al [41]. For this purpose, cells were removed from culture dishes by scraping.…”

Section: Lh Receptor Binding Studiesmentioning

confidence: 99%

Secretion of Steroids, Growth Factors, and Cytokines by Immortalized Mouse Granulosa Cell Lines1

Vanderstichele

Delaey

Winter

et al. 1994

Biology of Reproduction

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“…Although the existence of PRL receptors in bovine CL has been measured by others [32,33], the rbPL did not appear to be binding to PRL receptors in our membrane preparation from bovine CL. Whereas Saito and Saxena [32] and Poindexter et al [33] measured specific binding of human PRL to bovine CL, ovine PRL failed to bind bovine CL in a separate study [34]. Therefore, it appears that PRL receptors in the bovine CL may or may not be found when heterologous ligands are used.…”

Section: -Rbpl To Bovine CL (Total Binding [Solid Squares] Nonspecmentioning

confidence: 80%

Placental Lactogen and Somatotropin: Hormone Binding to the Corpus Luteum and Effects on the Growth and Functions of the Ovary in Heifers1

Lucy

Byatt

Curran

et al. 1994

Biology of Reproduction

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The effects of recombinant bovine placental lactogen (rbPL) and recombinant bovine somatotropin (rbST) on development of ovarian follicles and CL were tested in heifers. Estrus (day = 0) was synchronized and heifers were treated (Days 0-21) with either saline (control; n = 7), rbST (25 mg/day; n = 6), or rbPL. (50 mg/day; n = 8). Blood was collected daily for analyses of progesterone, estradiol, ST, PL, and insulin-like growth factor (IGF)-I; ultrasound was performed daily for measurement of follicles and CL. PL in plasma (mean +/- SE; ng/ml) averaged 4.1 +/- 0.2 for rbPL-treated heifers, and ST in plasma (ng/ml) averaged 2.7 +/- 0.3 for rbST-treated heifers. IGF-I in plasma (ng/ml) was increased for rbST-treated (198 +/- 10; p < 0.001) and rbPL-treated (143 +/- 9; p < 0.06) heifers compared to controls (117 +/- 9). After Day 9 of the estrous cycle, heifers treated with rbPL had larger CL (p < 0.001) and more progesterone in plasma (p < 0.001) than controls, whereas rbST-treated heifers were intermediate for these measures. Largest follicles were decreased in size (mm) throughout the estrous cycle for rbPL-treated heifers (12.9 +/- 0.4) compared to controls (14.2 +/- 0.5; p < 0.06) or heifers given rbST (14.0 +/- 0.5; p < 0.11). After Day 17 (preovulatory period), concentrations of estradiol in serum (pg/ml) were decreased for rbST-treated (2.7 +/- 0.3; p < 0.01) and rbPL-treated (2.9 +/- 0.2; p < 0.02) heifers compared to controls (3.8 +/- 0.3).(ABSTRACT TRUNCATED AT 250 WORDS)

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Failure of metoclopramide-induced acute hyperprolactinemia to affect time of ovulation or spontaneous luteolysis in the cycling mare

Becker¹,

Johnson²

1990

Journal of Equine Veterinary Science

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Absence of high-affinity binding sites for prolactin on the bovine corpus luteum

Cited by 3 publications

References 18 publications

Secretion of Steroids, Growth Factors, and Cytokines by Immortalized Mouse Granulosa Cell Lines1

Secretion of Steroids, Growth Factors, and Cytokines by Immortalized Mouse Granulosa Cell Lines1

Placental Lactogen and Somatotropin: Hormone Binding to the Corpus Luteum and Effects on the Growth and Functions of the Ovary in Heifers1

Failure of metoclopramide-induced acute hyperprolactinemia to affect time of ovulation or spontaneous luteolysis in the cycling mare

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