Absence of effect of power–frequency magnetic fields exposure on mouse embryonic lens development

Abstract

There are no reports in the literature investigating the biological effect of power-frequency (50/60 Hz) magnetic fields on embryonic lens development. We investigated whether power-frequency magnetic fields act as an environmental insult and induce changes in morphology or protein and/or transcription factor mRNA expression levels in developing mouse embryonic lenses. Three groups of pregnant mice were exposed to magnetic fields (50 Hz) of varying intensities (0.0, 1.5, and 4.5 mT) for 3 h per day from gestation day (GD) 0 to 18. The embryonic lenses were enucleated on GD 18. The lenses in the three groups were found to be transparent and the lens epithelial cells from the experimental groups exhibited normal ultrastructure comparable to the control. No significant differences were found among the three groups in quantity of water soluble protein (WSP), water insoluble protein (WIP), the ratio of WIP to total protein, and the mRNA expression levels of four well known developmentally regulated DNA-binding transcription factors: Pax6, Prox1, Sox1, and c-maf. We preserved one pregnant mouse per group, fed the neonatal mice until 2 weeks of age, and then found the lenses transparent. Our results did not detect any effects on the development of embryonic lenses in mice exposed to power-frequency magnetic fields.

Structural Changes Observed in Mice Liver Due to Chronic Exposure of 1900-2200 MHZ Radiations Emitted From Mobile Phone

“…Hepatocytes and Kupffer cells were seen in apoptosis form [16]. In contrary to the above findings, some reports stated that, there were no adverse biological effects of exposure to the non-ionizing radiation from the mobile phone: no breakage of double stranded DNA or effects on chromatin of rat brain [17], no effect on mouse embryonic lens development [18], no effect on psychomotor performance [19] and no histological changes on rat testis [20]. Keeping in view that, the increased number of mobile phone calls per day, length of each call, amount of time of usage of mobile phones and casual erection of the base stations in the residential areas, this study is designed to investigate the dose dependant effects of chronic exposure of 3G mobile phone induced EMFs in mice liver with reference to the structural changes at histological and biochemical level.…”

Differential effects of high and low strength magnetic fields on mouse embryonic development and vasculogenesis of embryonic stem cells

“…Increased congenital malformations were observed in the offspring of highvoltage switchyard workers which additionally suffered from fertility difficulties [76]. On the other hand, many studies described non-significant effects regarding the rate of embryonic death as well as teratogenic effects after exposure to MFs [77][78][79][80][81]. Recently, Mahram and Ghazavi found, that pregnant women and their unborn children exposed to ELF MFs at 0.31 ± 0.18 µT were not affected during pregnancy, regarding fetal growth and development [82].…”