Absence of Effect of Bovine Growth Hormone on Blood Sugar of Houssay Dogs

Sirek, Anna; Schoeffling, K; Webster, Monica; Sirek, O. V.

doi:10.1139/y64-035

Cited by 16 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…were hypophysectomized by the transbuccal technic as described. 4 Previous experiments from this laboratory have shown that little difference exists in the composition of aorta between the ages of six and eighteen months in mongrel dogs of either sex. 5 Completeness of the hypophysectomy was checked by histological examination of the removed tissue and by postmortem examination of the sella turcica.…”

Section: Methodsmentioning

confidence: 96%

Action of Growth Hormone and Thyroxine on Aortas of Hypophysectomized Dogs

Brosnan¹,

Sirek²,

Sirek³

et al. 1973

Diabetes

Self Cite

View full text Add to dashboard Cite

Young dogs were surgically hypophysectomized and maintained for eleven weeks postoperatively with age-matched normal controls. For three weeks prior to sacrifice, four hypophysectomized dogs were given daily injections of bovine growth hormone (GH, 0.2 mg./kg.) and another four were given daily injections of thyroxine (T 4 , 5 /tg-/ kg.). Aortas were removed, cleaned of adventitia and divided into three segments: arch, thoracic and abdominal. Each portion was analyzed for collagen, elastin, deoxyribonucleic acid (DNA), calcium, total mucopolysaccharides (MPS), hyaluronic acid (HA), heparan sulfate (HS), dermatan sulfate (DS) and chondroitin sulfate (GS). The arch and thoracic aortas of normal animals were found to contain more DNA, CS and elastin but less collagen than the abdominal aorta. Removal of the hypophysis resulted in an overall increase in elastin and DNA content and caused a decrease in all sulfated MPS. Administration of either CH or T 4 to hypophysectomized dogs had a profound effect on the majority of constituents in all segments of aorta. CH returned the content of elastin, DS and CS toward normal in at least two of the three aortic segments. T 4 returned the content of DNA, DS and CS toward normal in all segments. Moreover, T 4 treatment caused significant reductions in collagen and HA contents of thoracic and abdominal segments. These results indicate that: (1) the composition of normal aorta varies with the segment studied; (2) the composition of the aorta is markedly affected by hypophysectomy, CH and T 4 treatment; and (3) individual aortic segments show differential sensitivity to a given hormone. DIABETES 22:243-50, April, 1973. of the aortic wall and that replacement therapy with growth hormone (GH) partially corrects the metabolic defects. 1 ' 3 In the present paper we would like to give a more detailed account of the effects of GH than in our preliminary communications, and also to report on the effect of thyroxine (T 4 ), which was studied in our laboratory under comparable conditions. It was found that the action of T 4 on the aortic wall is different from but in some ways supplementary to that of GH. MATERIALS AND METHODSMongrel dogs of either sex between the ages of six and eighteen months and weighing between 8 and 12 kg. were hypophysectomized by the transbuccal technic as described. 4 Previous experiments from this laboratory have shown that little difference exists in the composition of aorta between the ages of six and eighteen months in mongrel dogs of either sex. 5 Completeness of the hypophysectomy was checked by histological examination of the removed tissue and by postmortem examination of the sella turcica. After surgery, dogs remained in the animal colony for eight weeks without hormone treatment. Then, four of the hypophysectomized dogs received subcutaneous injections of a highly purified bovine GH preparation (Wilhelmi, Lot no. 12, gift of the Endocrinology Study Section, N.I.H., Bethesda), 0.2 mg./kg./day for three weeks. Another four of the hypophysectomized dogs r...

show abstract

Section: Methodsmentioning

confidence: 96%

Action of Growth Hormone and Thyroxine on Aortas of Hypophysectomized Dogs

Brosnan¹,

Sirek²,

Sirek³

et al. 1973

Diabetes

Self Cite

View full text Add to dashboard Cite

show abstract

“…4 animals served as normal con trols and 24 were hypophysectomized by the transbuccal technique as described [16]. Completeness of the hypophysectomy was checked by histological examination of the removed tissue and by postmortem examination of the sella turcica.…”

Section: Methodsmentioning

confidence: 99%

Effect of Hormones on the Glycosaminoglycan Content of Canine Renal Arteries

Sirek¹,

Sirek²,

Cukerman³

1978

J Vasc Res

Self Cite

View full text Add to dashboard Cite

The effect of hypophysectomy and replacement therapy with individual hormones such as somatotropin, cortisone, estradiol, progesterone and testosterone on the glycosaminoglycan (GAG) composition of renal arteries was studied in young beagles. The renals from normal animals showed a uniformly low total GAG content of about two thirds of the value found for aorta and coronary arteries. Neither hypophysectomy nor hormone replacement altered the GAG content, with the exception of estradiol which showed a marked effect by raising the hyaluronic acid content to nearly double the normal value. These results by being at variance with those obtained for aorta and coronary arteries provide further evidence of the differential hormone sensitivity that exists in the various segments of the canine arterial tree with respect to GAG metabolism.

show abstract

“…Mongrel dogs of either sex, weighing approximately 10 kg each, were hypophysectomized by the transbuccal approach in pentobarbital (Nembutal) anesthesia (35 mg/kg) under aseptic conditions (3). Completeness of the hypophysectomy was checked by histo logical examination of the removed tissue and by postmortem examination of the sella turcica.…”

Section: Methodsmentioning

confidence: 99%

Failure of Ergotamine to Alter the Lipolytic Effect of Somatotropin in Dogs

Sirek¹,

Sirek²

1977

Pharmacology

View full text Add to dashboard Cite

Hypophysectomized dogs were injected intravenously with bovine somatotropin (GH), and plasma free fatty acid (FFA) concentrations were measured over a period of 2 h. The response consisted of an initial reduction in the concentration of plasma FFA followed by a rebound and a delayed rise above baseline values. When the animals were given a single intravenous injection of ergotamine tartrate 30 min prior to the administration of GH, the biphasic pattern with the sluggish rise in plasma FFA was retained. This finding is contrary to the results of identical experiments conducted by us with dihydroergotamine, which proved to be a potent amplifier of the lipolytic effect of GH. Since the only difference between these two compounds is the presence or absence of the double bond at C9 and C10 of the lysergic acid moiety, it appears that unless this bond is saturated the alkaloid is not capable of functioning as a biological amplifier.

show abstract

Absence of Effect of Bovine Growth Hormone on Blood Sugar of Houssay Dogs

Cited by 16 publications

References 0 publications

Action of Growth Hormone and Thyroxine on Aortas of Hypophysectomized Dogs

Action of Growth Hormone and Thyroxine on Aortas of Hypophysectomized Dogs

Effect of Hormones on the Glycosaminoglycan Content of Canine Renal Arteries

Failure of Ergotamine to Alter the Lipolytic Effect of Somatotropin in Dogs

Contact Info

Product

Resources

About