Absence of bacterial resistance following repeat exposure to photodynamic therapy

Pedigo, Lisa; Gibbs, Aaron; Scott, Robert J.; Street, Cale N.

doi:10.1117/12.822834

Cited by 37 publications

(35 citation statements)

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“…Due to its multi-target nature, and therefore low probability of triggering the development of resistance in (micro)organisms [38][39][40][41], this therapy has been tested in various research areas as an alternative approach to actual methods to control insect pests, water quality, microbiological food quality; and also in the disinfection and sterilization of materials and surfaces in different contexts (industrial, household and hospital). Furthermore, the use of this form of eradicating microbes or noxious organisms becomes increasingly achievable in practice if one thinks that, for certain purposes, the PS may be immobilized on inert solid supports allowing their reuse and recycling, making this technology of broad-spectrum activity, economic, sustainable, durable, and environmentally friendly.…”

Section: Introductionmentioning

confidence: 99%

Potential applications of porphyrins in photodynamic inactivation beyond the medical scope

Alves

Faustino

Neves

et al. 2015

Journal of Photochemistry and Photobiology C: Photochemistry Re

191

128

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a b s t r a c tAlthough the discovery of light-activated antimicrobial agents had been reported in the 1900s, only more recently research work has been developed toward the use of photodynamic process as an alternative to more conventional methods of inactivation of micro(organisms). The photoprocess causes cell death through irreversible oxidative damage by reactive oxygen species produced by the interaction between a photosensitizing compound and a light source.With great emphasis on the environmental area, photodynamic inactivation (PDI) has been tested in insect eradication and in water disinfection. Lately, other studies have been carried out concerning its possible use in aquaculture waters or to the control of food-borne pathogens. Other potential applications of PDI in household, industrial and hospital settings have been considered.In the last decade, scientific research in this area has gained importance not only due to great developments in the field of materials chemistry but also because of the serious problem of the increasing number of bacterial species resistant to common antibiotics. In fact, the design of antimicrobial surfaces or selfcleaning materials is a very appealing idea from the economic, social and public health standpoints. Thus, PDI of micro(organisms) represents a promising alternative.In this review, the efforts made in the last decade in the investigation of PDI of (micro)organisms with potential applications beyond the medical field will be discussed, focusing on porphyrins, free or immobilized on solid supports, as photosensitizing agents.

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Section: Introductionmentioning

confidence: 99%

Potential applications of porphyrins in photodynamic inactivation beyond the medical scope

Alves

Faustino

Neves

et al. 2015

Journal of Photochemistry and Photobiology C: Photochemistry Re

191

128

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“…The reactivity of singlet oxygen with organic molecules, however, is indiscriminate, therefore any macromolecule of the bacterial cell is a potential target. This potential multiplicity of, and lack of specificity towards, targets makes the development of resistance by bacteria more difficult [63], and indeed despite a number of attempts to induce bacterial resistance with sub-lethal PACT, no resistant organisms have yet been reported [64][65][66][67], although upregulation of a heat-shock protein has been noted in some organisms [69,70] . This is of particular significance in light of a recent World Health Organisation (WHO) report highlighting the global problem of antibiotic resistance in bacteria [68].…”

Section: Photodynamic Antimicrobial Chemotherapymentioning

confidence: 99%

Photosensitisers – the progression from photodynamic therapy to anti-infective surfaces

Craig

McCoy

Gorman³

et al. 2014

Expert Opinion on Drug Delivery

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Introduction: The application of light as a stimulus in pharmaceutical systems and the associated ability to provide precise spatiotemporal control over location, wavelength and intensity, allowing ease of external control independent of environmental conditionals, has led to its increased use. Of particular note is the use of light with photosensitisers.Areas covered: Photosensitisers are widely used in photodynamic therapy to cause a cidal effect towards cells on irradiation due to the generation of reactive oxygen species. These cidal effects have also been employed to treat infectious diseases.The effects and benefits of photosensitisers in the treatment of such conditions are still being developed and further realised, with the design of novel delivery strategies.This review provides an overview of the realisation of the pharmaceutically relevant uses of photosensitisers both in the context of current research, and in terms of current clinical application, and looks to the future direction. ! 2!Expert opinion: Substantial advances have been and are being made in the use of photosensitisers. Of particular note are their antimicrobial applications, due to absence of resistance that is so frequently associated with conventional treatments.Their potency of action, and the ability to immobilise to polymeric supports is opening a wide range of possibilities with great potential for use in healthcare infection prevention strategies.

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“…Without irradiation, photosensitizer does not exert cytotoxic effect, indicating that residual toxicity after the treatment is negligible. Furthermore, it is suggested that the PDT does not induce bacterial resistance because singlet oxygen oxidize bacterial cell components non-selectively [26,27]. This is a major advantage in comparison with antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Microbicidal Activity of Artificially Generated Hydroxyl Radicals

Sheng

Nakamura

Kanno

et al. 2015

Interface Oral Health Science 2014

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The hydroxyl radical, one of the reactive oxygen species, has one unpaired electron in the structure, so that it tends to deprive other substances of an electron which is so-called oxidation. It is known that hydroxyl radicals produced by immunological response kill invading microorganisms by the oxidation. Besides the immune system, it has been demonstrated that hydroxyl radicals play an important role in the bactericidal action of antibiotics. In this context, we have conducted a research to develop disinfection techniques utilizing artificially generated hydroxyl radicals. We adopted photolysis of H 2 O 2 , sonolysis of water and the other photo-chemical reaction as generators of hydroxyl radicals. A series of studies demonstrated that the microbicidal activity of hydroxyl radicals was sufficient to kill bacteria in an experimental biofilm as well as planktonic bacteria and fungi within a short-treatment time. In addition, the safety aspect is confirmed by an in vivo study and a literature review. Thus, it is suggested that disinfection treatment utilizing artificially generated hydroxyl radicals can be applicable to medical/dental therapy as novel disinfection treatments.

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Absence of bacterial resistance following repeat exposure to photodynamic therapy

Cited by 37 publications

References 0 publications

Potential applications of porphyrins in photodynamic inactivation beyond the medical scope

Potential applications of porphyrins in photodynamic inactivation beyond the medical scope

Photosensitisers – the progression from photodynamic therapy to anti-infective surfaces

Microbicidal Activity of Artificially Generated Hydroxyl Radicals

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