Absence of Activated Murine Leukaemia Virus in X-irradiated CBA/H-T6Crc Mice

Holmes, Harvey; Tuffrey, Maureen; Wilson, Lynette; Barnes, R. D.

doi:10.1099/0022-1317-53-2-377

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…These recombinants can be detected in the preleukemic period (Sankar-Mistry and Jolicoeur, 1980), are frequently isolated from lymphoma cells serially passaged in vitro and in vivo, but are paradoxically seldom recovered from the primary tumors (Ihle et al, 1976;Lieberman et al, 1976Lieberman et al, , 1977Lieberman et al, , 1979Sankar-Mistry and Jolicoeur et al, 1980). Furthermore, radiation-induced TL were obtained in CBA mice which do not express endogenous retroviruses, and also, in several instances, the development of radioleukemia did not correlate (positively or negatively) with derepression of endogenous MuLV (Holmes et al, 1981;Mayer and Dorsch-Hasler, 1982). These conflicting data urged for a revision of the originally proposed mechanism: radiation-activation of endogenous viruses resulting in the production of leukemo-genic recombinants and finally in leukemia induction.…”

Section: Induction Of Thymic Lymphosarcomas In C57blh Mice After Inocmentioning

confidence: 99%

Induction of thymic lymphosarcomas in C57BL/6 mice after inoculation of weakly oncògenic viruses associated with a sub‐leukemogenic radiation exposure (1.75 GY × 2)

Galiay

Legrand

Astier-Gin

et al. 1986

Intl Journal of Cancer

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B-ecotropic retroviruses arise frequently in old or irradiated C57BL/6 mice as a consequence of a genetic recombination between endogenous eco- and xenotropic retroviruses. They are weakly oncogenic and express a very low tropism for thymic cells. However, their activation by X-rays and the subsequent insertion of new proviral sequences in the cell genome of in vivo- and in vitro-passaged tumors suggest that they might play a role in radioleukemogenesis. To study this possibility, a cloned B-ecotropic virus (1223) was injected into C57BL/6 mice subjected to a subleukemogenenic irradiation which induces only 7% of thymic lymphosarcomas (TL). When it was injected prior to or after irradiation, 1223 induced respectively 31% and 19% of TL. The incidence of TL in the different groups closely correlated with virus expression in hematopoietic tissues during the preleukemic period. Thus, irradiation seems to amplify bone marrow (BM) and thymic cell population(s) which play a decisive role in viral expression. A recombinant provirus (presumably the injected 1223) was detected in the genomic DNA of all tumors tested irrespective of the inductive protocol. BM restoration, which does not inhibit TL produced by highly oncogenic passaged viruses, but prevents the development of TL induced by 4 doses of 1.75 Gy, also provided strong protection in the present experiments. The present data support the hypothesis whereby weakly oncogenic B-ecotropic viruses similar to those activated by radiation might be involved in the development of TL.

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Section: Induction Of Thymic Lymphosarcomas In C57blh Mice After Inocmentioning

confidence: 99%

Induction of thymic lymphosarcomas in C57BL/6 mice after inoculation of weakly oncògenic viruses associated with a sub‐leukemogenic radiation exposure (1.75 GY × 2)

Galiay

Legrand

Astier-Gin

et al. 1986

Intl Journal of Cancer

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RNA virus expression during and after methylnitrosourea-induced T-cell leukemogenesis in mice

Grompe

Kreja

Schmidt³

et al. 1985

Blut

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The expression of RNA tumor virus was studied in BDF 1 mice after leukemogenic treatment with a single dose of methylnitrosourea (MNU) and in leukemic thymuses by a cell ELISA using antibodies against the viral glycoprotein gp 70 and by co-culture for the detection of eco- and xenotropic virus. The majority of the thymomas were positive for gp 70; ecotropic, but not xenotropic infectious virus could be detected in some of them. Early after MNU application the thymus and the bone marrow were positive for gp 70 in some animals. Later, after a phase with positive results with spleen cells, the bone marrow and the spleen were negative again. Only the thymus of some mice were positive during the last weeks before the first leukemias appeared.

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Activation of endogenous C-type retroviral genomes by internal alpha-irradiation of mice with224Radium

Schmidt

Erfle

Müller

1985

Radiat Environ Biophys

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Sensitive cocultivation techniques were applied to study the radiation-induced activation of endogenous retroviral genomes in different mouse strains by the alpha-emitting radionuclide 224Radium. Activated infectious C-type retroviruses were detected in spleen, bone marrow and bone tissues of C57BL/6-, BALB/c- and NMRI mice. The titres of high-dose-irradiated animals were higher than those found in low-dose-irradiated animals. Infectious retrovirus could be detected with a dose of 13.2 rad (maximum dose rate 0.9 rad/day) in the skeleton, and a dose of 4.2 rad (maximum dose rate 0.3 rad/day) in the spleen. The virus activation pattern was different in the three mouse strains. These data indicate that activation of endogenous retroviral genomes by alpha-irradiation shows a dose-effect relationship and a dependence on the genetic background of the mouse.

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Absence of Activated Murine Leukaemia Virus in X-irradiated CBA/H-T6Crc Mice

Cited by 3 publications

References 13 publications

Induction of thymic lymphosarcomas in C57BL/6 mice after inoculation of weakly oncògenic viruses associated with a sub‐leukemogenic radiation exposure (1.75 GY × 2)

Induction of thymic lymphosarcomas in C57BL/6 mice after inoculation of weakly oncògenic viruses associated with a sub‐leukemogenic radiation exposure (1.75 GY × 2)

RNA virus expression during and after methylnitrosourea-induced T-cell leukemogenesis in mice

Activation of endogenous C-type retroviral genomes by internal alpha-irradiation of mice with224Radium

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