Abscisic acid pretreatment enhances salt tolerance of rice seedlings: Proteomic evidence

Li, Xiaojuan; Yang, Mo; Chen, Hui; Qu, Le-Qing; Chen, Fan; Shen, Shihua

doi:10.1016/j.bbapap.2010.01.004

Cited by 138 publications

(107 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nipponbare) were germinated at 30°C for 48 h. The germinated seedlings were grown in a greenhouse at 28°C ± 2°C during the day and 22°C ± 2°C at night under natural light conditions (14 h light/10 h darkness period). Seedlings were grown in nutrient solution according to Li et al [38]. For high temperature treatments, three-week-old rice seedlings were exposed to 42°C (Treatment) or 28°C (Control) for 12 h and 24 h [8].…”

Section: Methodsmentioning

confidence: 99%

Phosphoproteins regulated by heat stress in rice leaves

Chen

Zhang

et al. 2011

Proteome Sci

View full text Add to dashboard Cite

BackgroundHigh temperature is a critical abiotic stress that reduces crop yield and quality. Rice (Oryza sativa L.) plants remodel their proteomes in response to high temperature stress. Moreover, phosphorylation is the most common form of protein post-translational modification (PTM). However, the differential expression of phosphoproteins induced by heat in rice remains unexplored.MethodsPhosphoprotein in the leaves of rice under heat stress were displayed using two-dimensional electrophoresis (2-DE) and Pro-Q Diamond dye. Differentially expressed phosphoproteins were identified by MALDI-TOF-TOF-MS/MS and confirmed by Western blotting.ResultsTen heat-phosphoproteins were identified from twelve protein spots, including ribulose bisphos-phate carboxylase large chain, 2-Cys peroxiredoxin BAS1, putative mRNA binding protein, Os01g0791600 protein, OSJNBa0076N16.12 protein, putative H(+)-transporting ATP synthase, ATP synthase subunit beta and three putative uncharacterized proteins. The identification of ATP synthase subunit beta was further validated by Western-blotting. Four phosphorylation site predictors were also used to predict the phosphorylation sites and the specific kinases for these 10 phosphoproteins.ConclusionHeat stress induced the dephosphorylation of RuBisCo and the phosphorylation of ATP-β, which decreased the activities of RuBisCo and ATP synthase. The observed dephosphorylation of the mRNA binding protein and 2-Cys peroxiredoxin may be involved in the transduction of heat-stress signaling, but the functional importance of other phosphoproteins, such as H+-ATPase, remains unknown.

show abstract

Section: Methodsmentioning

confidence: 99%

Phosphoproteins regulated by heat stress in rice leaves

Chen

Zhang

et al. 2011

Proteome Sci

View full text Add to dashboard Cite

show abstract

“…Activities of individual APX isoforms are related to increased generation of ROS during salt stress. In addition, their biochemical characterizations show different patterns in terms of properties such as substrate specificity, optimal pH and ascorbate depletion [3,23,38]. When PAGE analysis of leaf APX isoforms was carried out, it revealed seven isoforms and particularly, an increase in APX-6 activity at the NaCl concentration of 300 mM (Fig.…”

Section: Discussionmentioning

confidence: 99%

Responses of Photosynthetic Efficiency and Ascorbate Peroxidase Induced by Salt Stress in Rice (Oryza sativa L.)

Koo¹,

Im²,

Chun³

et al. 2010

Journal of Life Science

View full text Add to dashboard Cite

We investigated changes in photosynthesis and activity of ascorbate peroxidase (APX) that scavenges ROS as responses to oxidative stress induced by salinity in rice (Oryza sativa L.). Photosynthetic efficiency of rice leaves, monitored in terms of Fv/Fm, declined with the increase of salt concentration (100-300 mM NaCl). Salinity caused an increase of H2O2 in leaves of rice, with an increase of APX activity. Among total APX isoforms, an isoform of stromal-APX 1 in leaves of rice was completely inactivated by 300 mM NaCl, but was not affected by chilling or drought. The results suggest that salt stress acts in quite a different mechanism in relation to the activity of stromal-APX from that of other stresses such chilling and drought. We carried out RT-PCR for analysis of genes expression of APX isoforms as affected by salt stress. The expression of cytosolic APX / thylakoid-bound APX genes in leaves of rice exposed to salt stress was increased, while stromal APX gene expression rapidly declined.Key words : Rice, salt stress, ascorbate peroxidase (APX), stromal APX gene, Fv/Fm *Corresponding author *Tel：+82-51-890-1525, Fax：+82-51-890-1521 Introduction Abiotic stress such as drought and salinity causes considerable damages to crop worldwide [4,8,31]. When plants are exposed to abiotic stresses, they experience functional disorder of metabolism including oxidative stress, osmotic stress and ionic imbalance [13,20,32]. Salinity induces oxidative stresses in plants by increasing reactive oxygen species (ROS) such as superoxide, hydrogen peroxide and hydroxyl radical [11,26] APX can easily eliminate H2O2 with high affinity in the cytosol, chloroplasts (stroma and thylakoid membrane) and other subcellular locations (microbody) [9,16,37]. These APX isoforms have been separated from spinach chloroplasts, tea leaves and plastids of tobacco, followed by many studies on their enzymatic/molecular properties [6,29,35]. The significant differences of those isoforms are apparent in their physico-chemical and kinetic properties. Compared to cytosolic form, chloroplastic isoforms (tAPX and sAPX) have very short life time in the culture medium without ascorbic acid (AsA) and their sensitivity to thiol reagents as well as to inhibitors (such as hydroxylamine) is much higher than their cytosolic counterparts [6,7]. ROS metabolism in a particular compartment can effect the different cellular compartments. The application of light stress to Arabidopsis has been reported in the induction of cytosolic. But not induce chloroplastic ROS removal enzymes [10,18,19,33].Several studies showed that activity of cytosolic/chloroplastic APX is important in protecting plant cells from oxida- 생명과학회지 2010, Vol. 20. No. 8 tive stress, which is one of many dysfunctions caused by salt stress [3,40]. We investigated changes in photochemical efficiency, activity of ascorbate peroxidase (APX) as well as its mRNA expression induced by oxidative stress in rice leaves that had been exposed to salinity. The observations suggested that a salinity-induced in...

show abstract

“…Bitkiler katalazların izozimlerine sahiptir: CAT-1 ve CAT-2 peroksizomlarla, CAT-3 ise mitokondri ile ilişkilidir. Proteomik çalışmalar, tuz stresi koşullarında Oryza sativa'da CAT seviyelerinin arttığını [39,50]; fakat Citrus aurantium, Hordeum vulgare ve Cucumis sativus'da azaldığını göstermiştir [38,51,52].…”

Section: Rot'lara Karşı Savunma Sistemleriunclassified

Tuz Stresi Altındaki Bitkilerin Metabolik Yollarındaki Proteom Değişimleri

Yıldız¹,

Terzi²,

Akçalı³

2014

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

View full text Add to dashboard Cite

ÖzetBitkilerde tuz stresine tolerans transkriptom, proteom ve metabolom kompozisyonundaki değişikliklere eşlik eden gen ekspresyonundaki önemli değişiklikleri kapsamaktadır. Proteinler direkt olarak stres cevaplarında fonksiyon görmektedir. Bu nedenle, proteomik çalışmalar tuz stresine tolerans ve fonksiyonel proteinler arasındaki muhtemel ilişkileri açıklayabilmektedir. Tuz stresine cevapta fonksiyon gören proteinlerin teşhisi tuza toleranslı transgenik bitkilerin geliştirilmesinde önemlidir. Bu derlemede, bitkilerin tuz stresine cevaplarını analiz etmek için yapılan son proteomik çalışmalar, tuz stresine cevapta muhtemel mekanizmalar ve metabolik yollarda fonksiyon gören proteinlerin seviyesindeki değişimler tartışılmıştır.Anahtar Kelimeler: Bitki, Tuz toleransı, Proteomik. Proteom Changes in Metabolic Pathways of Plants under Salt Stress AbstractTolerance against salt stress in plants includes substantial changes in gene expression which is accompanied with changes in composition of transcriptome, metabolome and proteome. Proteins are directly involved in plant stress response. Therefore, proteomics studies can explain the possible relationships between salinity tolerance and functional proteins. The identification of the proteins that are involved in responses to salinity stress could lead to the development of transgenic plants that have an enhanced tolerance to salinity. In this review, the recent proteomics studies to the analysis of the responses of plants to salinity stress, the possible mechanisms in response to salinity stress and the changes in the level of proteins involved in metabolic pathways are discussed.

show abstract

Abscisic acid pretreatment enhances salt tolerance of rice seedlings: Proteomic evidence

Cited by 138 publications

References 62 publications

Phosphoproteins regulated by heat stress in rice leaves

Phosphoproteins regulated by heat stress in rice leaves

Responses of Photosynthetic Efficiency and Ascorbate Peroxidase Induced by Salt Stress in Rice (Oryza sativa L.)

Tuz Stresi Altındaki Bitkilerin Metabolik Yollarındaki Proteom Değişimleri

Contact Info

Product

Resources

About