Abscisic acid-mediated induction of FLAVIN-CONTAINING MONOOXYGENASE 2 leads to reduced accumulation of methylthioalkyl glucosinolates in Arabidopsis thaliana

Li, Yimeng; Li, Rui; Sawada, Yuji; Boerzhijin, Surina; Kuwahara, Ayuko; Sato, Muneo; Hirai, Masami Yokota

doi:10.1016/j.plantsci.2020.110764

Cited by 6 publications

(8 citation statements)

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“…We hypothesize that iron homeostasis is maintained through the regulatory role of basic helix–loop–helix (bHLH) TFs, which may represent one of the mechanisms underlying the salt tolerance exhibited by CK-signaling mutants. MYB29 and MYB76 are also positive regulators of aliphatic glucosinolate (e.g., 4-methylthiobutyl [4MT]-GSL) biosynthesis in Arabidopsis ( 84 ), and were down-regulated in the CK-signaling mutants ( Fig. 4 ).…”

Section: Discussionmentioning

confidence: 99%

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Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis

Abdelrahman

Nishiyama

Tran

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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Cytokinin (CK) in plants regulates both developmental processes and adaptation to environmental stresses. Arabidopsis histidine phosphotransfer ahp2,3,5 and type-B Arabidopsis response regulator arr1,10,12 triple mutants are almost completely defective in CK signaling, and the ahp2,3,5 mutant was reported to be salt tolerant. Here, we demonstrate that the arr1,10,12 mutant is also more tolerant to salt stress than wild-type (WT) plants. A comprehensive metabolite profiling coupled with transcriptome analysis of the ahp2,3,5 and arr1,10,12 mutants was conducted to elucidate the salt tolerance mechanisms mediated by CK signaling. Numerous primary (e.g., sugars, amino acids, and lipids) and secondary (e.g., flavonoids and sterols) metabolites accumulated in these mutants under nonsaline and saline conditions, suggesting that both prestress and poststress accumulations of stress-related metabolites contribute to improved salt tolerance in CK-signaling mutants. Specifically, the levels of sugars (e.g., trehalose and galactinol), amino acids (e.g., branched-chain amino acids and γ-aminobutyric acid), anthocyanins, sterols, and unsaturated triacylglycerols were higher in the mutant plants than in WT plants. Notably, the reprograming of flavonoid and lipid pools was highly coordinated and concomitant with the changes in transcriptional levels, indicating that these metabolic pathways are transcriptionally regulated by CK signaling. The discovery of the regulatory role of CK signaling on membrane lipid reprogramming provides a greater understanding of CK-mediated salt tolerance in plants. This knowledge will contribute to the development of salt-tolerant crops with the ability to withstand salinity as a key driver to ensure global food security in the era of climate crisis.

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Section: Discussionmentioning

confidence: 99%

“…S2 D and Dataset S5 ). Additionally, glucosinolate levels are negatively regulated by ABA signaling ( 84 ). Thus, the decrease in 3-MSOP-GLS is in agreement with the previous reports of increased ABA responsiveness in ahp2,3,5 and arr1,10,12 mutants ( 17 , 21 ).…”

Section: Discussionmentioning

confidence: 99%

Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis

Abdelrahman

Nishiyama

Tran

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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“…In addition, in the Glu-insensitive mutants, the effects of JA and Glu on the GSL content reduced significantly, suggesting that JA and Glu signaling is involved in cross-talk in regulating GSL biosynthesis. Moreover, Glu upregulates GSLs via the ABA signaling pathway and decreased accumulation of 4-methylthiobutyl ( Li et al, 2021 ). Same with Glu, the contents of total GSLs, glucoiberin, glucoraphanin, glucobrassicin, sulforaphane, glucobrassicin, and neoglucobrassicin in cabbage ( B. oleracea L. var.…”

Section: Glucose and Sucrosementioning

confidence: 99%

Effects of Plant Hormones, Metal Ions, Salinity, Sugar, and Chemicals Pollution on Glucosinolate Biosynthesis in Cruciferous Plant

Liu

Wang

et al. 2022

Front. Plant Sci.

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Cruciferous vegetable crops are grown widely around the world, which supply a multitude of health-related micronutrients, phytochemicals, and antioxidant compounds. Glucosinolates (GSLs) are specialized metabolites found widely in cruciferous vegetables, which are not only related to flavor formation but also have anti-cancer, disease-resistance, and insect-resistance properties. The content and components of GSLs in the Cruciferae are not only related to genotypes and environmental factors but also are influenced by hormones, plant growth regulators, and mineral elements. This review discusses the effects of different exogenous substances on the GSL content and composition, and analyzes the molecular mechanism by which these substances regulate the biosynthesis of GSLs. Based on the current research status, future research directions are also proposed.

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“…Several studies have been carried out to identify and/or characterize the FMO genes belonging to the auxin biosynthetic clade (named YUCCAs) in plants [ 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. However, only very few reports are available for the other two clades [ 21 , 39 , 40 , 41 , 42 , 43 , 44 ]. In addition to the previously reported roles, studies indicate the role of FMOs in abiotic stresses as well.…”

Section: Introductionmentioning

confidence: 99%

Genomic Survey of Flavin Monooxygenases in Wild and Cultivated Rice Provides Insight into Evolution and Functional Diversities

Gaba

Bhowal

Pareek

et al. 2023

IJMS

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The flavin monooxygenase (FMO) enzyme was discovered in mammalian liver cells that convert a carcinogenic compound, N-N′-dimethylaniline, into a non-carcinogenic compound, N-oxide. Since then, many FMOs have been reported in animal systems for their primary role in the detoxification of xenobiotic compounds. In plants, this family has diverged to perform varied functions like pathogen defense, auxin biosynthesis, and S-oxygenation of compounds. Only a few members of this family, primarily those involved in auxin biosynthesis, have been functionally characterized in plant species. Thus, the present study aims to identify all the members of the FMO family in 10 different wild and cultivated Oryza species. Genome-wide analysis of the FMO family in different Oryza species reveals that each species has multiple FMO members in its genome and that this family is conserved throughout evolution. Taking clues from its role in pathogen defense and its possible function in ROS scavenging, we have also assessed the involvement of this family in abiotic stresses. A detailed in silico expression analysis of the FMO family in Oryza sativa subsp. japonica revealed that only a subset of genes responds to different abiotic stresses. This is supported by the experimental validation of a few selected genes using qRT-PCR in stress-sensitive Oryza sativa subsp. indica and stress-sensitive wild rice Oryza nivara. The identification and comprehensive in silico analysis of FMO genes from different Oryza species carried out in this study will serve as the foundation for further structural and functional studies of FMO genes in rice as well as other crop types.

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Abscisic acid-mediated induction of FLAVIN-CONTAINING MONOOXYGENASE 2 leads to reduced accumulation of methylthioalkyl glucosinolates in Arabidopsis thaliana

Cited by 6 publications

References 58 publications

Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis

Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis

Effects of Plant Hormones, Metal Ions, Salinity, Sugar, and Chemicals Pollution on Glucosinolate Biosynthesis in Cruciferous Plant

Genomic Survey of Flavin Monooxygenases in Wild and Cultivated Rice Provides Insight into Evolution and Functional Diversities

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