Abscisic acid levels of individual leaf cells

Brinckmann, Enno; Hartung, Wolfram; Wartinger, Margarete

doi:10.1034/j.1399-3054.1990.800108.x

Cited by 5 publications

(5 citation statements)

References 8 publications

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“…1988), as it does when epidermal peels are water stressed (Cornish & Zeevaart 1986). In addition, other reports corroborate a high level of ABA in the guard‐cell symplast (0·2 m m , Lahr & Raschke 1988; 6·5 m m , Brinckmann, Hartung & Wartinger 1990). In the present experiments, the guard‐cell symplastic ABA content was nominally 2–3 µ m (Fig.…”

Section: Discussionsupporting

confidence: 64%

Abscisic acid introduced into the transpiration stream accumulates in the guard‐cell apoplast and causes stomatal closure

Zhang¹,

Outlaw²

2001

Plant Cell & Environment

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Petioles of water-sufficient intactUnlike when leaves are stressed, the guard-cell symplast ABA content did not increase because of ABA infusion ( P = 0·48; 3·0 ± ± ± ± 0·5 versus 4·0 ± ± ± ± 1·2 fg guard-cell-pair ----1 ).However, the guard-cell apoplast ABA content increased linearly ( R 2 = 0·98) from ----0·2 ± ± ± ± 0·5 to 3·1 ± ± ± ± 1·3 fg guard-cellpair ----1 ( ª ª ª ª 3·1 m m m m M ) and was inversely related to leaf conductance ( R 2 = 0·82). Apparently, xylem ABA accumulates in the guard-cell wall as a result of evaporation of the apoplast solution. This mechanism provides for integrating transpiration rate and ABA concentration in the xylem solution.

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Section: Discussionsupporting

confidence: 64%

Abscisic acid introduced into the transpiration stream accumulates in the guard‐cell apoplast and causes stomatal closure

Zhang¹,

Outlaw²

2001

Plant Cell & Environment

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“…The concentration of ABA within a Commelina leaf's guard cells was measured by Brinckmann et al (1990) to be about 2.5 µ M but this was after solvent extraction which would have combined the free and fettered fractions. As over 90% of the ABA in an unwilted bean leaf is in the fettered fraction (Loveys 1977), this amount of ABA would be capable of initiating synthesis of stress ABA on wilting.…”

Section: Discussionmentioning

confidence: 99%

Initiation of the synthesis of ‘stress’ ABA by (+)‐[²H₆]ABA infiltrated into leaves of Commelina communis

Georgopoulou

Milborrow

2012

Physiologia Plantarum

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The 'fettered' fraction of abscisic acid (ABA) that is held within the chloroplasts of unwilted bean and Commelina communis leaves is released when the leaves wilt and it is this 'free' ABA that is now proposed to cause the stomata to close within 2 or 3 min, well before the rise in total ABA can be detected. The large increase in 'stress' ABA begins 2-3 h later. The fettered ABA in a centrifuged homogenate is released by hyperosmotic solutions of mannitol (0.8 M) and NaCl (0.4 M). Dilute solution of halothane (10 mM) and colchicine (1 mM), the detergent sodium dodecyl sulphate (1 mM) the herbicide 2,4-d (0.1 mM) and dithiothreitol (0.01 mM) also caused ABA to be released. Zeatin (0.01 mM), cumene hydroperoxide (0.01 mM) and CaCl(2) (1 mM) had negligible effects. It was postulated that the ABA released from the chloroplasts by wilting could be the signal that initiates the synthesis of the dioxygenase and other enzymes necessary to produce the rise (up to 40-fold) in the amount of stress ABA that is seen 2-3 h later. To test this hypothesis, a solution of (+)-[(2) H(6) ]ABA was vacuum infiltrated into unwilted Commelina leaves to mimic the rise in ABA caused by wilting and gas chromatography/mass spectrometry of the ABA in the extract after 3 h showed that concentrations of (+)-[(2) H(6) ]ABA of up to 0.3 µM stimulated synthesis of endogenous [(1) H]ABA by 15-fold in the unwilted leaves. A 0.5 µM solution blocked the increase in the amount of ABA formed and also reduced the amount of ABA formed in response to a 0.8 M mannitol solution.

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“…Because the ABA-induced [Ca2+Ii transients only last 2 to 5 min, this provides a possible upper limit to a transit time of free ABA in the cytosol. Evidence for avacuolar transport system for protonated ABA exists in Commelina, and guard cells can accumulate ABA up to a concentration of 6 mM (Baier et al, 1990; Brinckmann et al, 1990). Therefore, release of ABA inside the cell may lead to its rapid removal to the vacuole and thus effective inactivation.…”

Section: Response To Previous Growth Temperaturementioning

confidence: 99%

Two Transduction Pathways Mediate Rapid Effects of Abscisic Acid in Commelina Guard Cells.

et al. 1994

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Commelina guard cells can be rapidly closed by abscisic acid (ABA), and it is thought that this signal is always transduced through increases in cytosolic calcium. However, when Commelina plants were grown at 10 to 17OC, most guard cells failed to exhibit any ABA-induced increase in cytosolic calcium even though all of these cells closed. At growth temperatures of 25OC ar above, ABA-induced closure was always associated with an increase in cytosolic calcium. This suggests that there may be two transduction mutes for ABA in guard cells; only one involves increases in cytosolic calcium. Activation of either pathway on its own appears to be sufficient to cause closure. Because the rates of ABA accumulation and transport in plants grown at different temperatures are likely to be different, we synthesized and microinjected caged ABA directly into guard cells. ABA was released internally by UV photolysis and subsequently caused stomatal closure. This result suggests a possible intracellular locale for the hypothesized ABA receptor.

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Abscisic acid levels of individual leaf cells

Cited by 5 publications

References 8 publications

Abscisic acid introduced into the transpiration stream accumulates in the guard‐cell apoplast and causes stomatal closure

Abscisic acid introduced into the transpiration stream accumulates in the guard‐cell apoplast and causes stomatal closure

Initiation of the synthesis of ‘stress’ ABA by (+)‐[²H₆]ABA infiltrated into leaves of Commelina communis

Two Transduction Pathways Mediate Rapid Effects of Abscisic Acid in Commelina Guard Cells.

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Abscisic acid levels of individual leaf cells

Cited by 5 publications

References 8 publications

Abscisic acid introduced into the transpiration stream accumulates in the guard‐cell apoplast and causes stomatal closure

Abscisic acid introduced into the transpiration stream accumulates in the guard‐cell apoplast and causes stomatal closure

Initiation of the synthesis of ‘stress’ ABA by (+)‐[2H6]ABA infiltrated into leaves of Commelina communis

Two Transduction Pathways Mediate Rapid Effects of Abscisic Acid in Commelina Guard Cells.

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Initiation of the synthesis of ‘stress’ ABA by (+)‐[²H₆]ABA infiltrated into leaves of Commelina communis