Abscisic acid-induced freezing tolerance in the mossPhyscomitrella patens is accompanied by increased expression of stress-related genes

Minami, Anzu; Nagao, Manabu; Arakawa, Keita; Fujikawa, Seizo; Takezawa, Daisuke

doi:10.1078/0176-1617-00888

Cited by 73 publications

(69 citation statements)

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“…Ethidium bromidestained rRNA for each sample is also shown. the cells are damaged by freezing, from 22°C to 210°C in protonemata of P. patens (Minami et al, 2003;Nagao et al, 2005). Thus, ABA-treated protonemata of nontransgenic and transgenic moss lines were subjected to freezing to 25°C, and after thawing, survival was determined by regrowth on agar medium.…”

Section: Changes In Osmotic and Freezing Stress Tolerance In Transgenmentioning

confidence: 99%

“…Pretreatment with ABA has been shown to be effective for cryopreservation of several moss species (Pence, 1998). We previously reported that the moss Physcomitrella patens increases freezing tolerance upon ABA treatment with increased expression of stress-related genes (Minami et al, 2003(Minami et al, , 2006. Analysis of endogenous ABA in P. patens revealed that hyperosmotic stress treatment of its protonemata increases the levels of endogenous ABA .…”

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Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

et al. 2010

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Abscisic acid (ABA) is postulated to be a ubiquitous hormone that plays a central role in seed development and responses to environmental stresses of vascular plants. However, in liverworts (Marchantiophyta), which represent the oldest extant lineage of land plants, the role of ABA has been least emphasized; thus, very little information is available on the molecular mechanisms underlying ABA responses. In this study, we isolated and characterized MpABI1, an ortholog of ABSCISIC ACID INSENSITIVE1 (ABI1), from the liverwort Marchantia polymorpha. The MpABI1 cDNA encoded a 568-amino acid protein consisting of the carboxy-terminal protein phosphatase 2C (PP2C) domain and a novel amino-terminal regulatory domain. The MpABI1 transcript was detected in the gametophyte, and its expression level was increased by exogenous ABA treatment in the gemma, whose growth was strongly inhibited by ABA. Experiments using green fluorescent protein fusion constructs indicated that MpABI1 was mainly localized in the nucleus and that its nuclear localization was directed by the aminoterminal domain. Transient overexpression of MpABI1 in M. polymorpha and Physcomitrella patens cells resulted in suppression of ABA-induced expression of the wheat Em promoter fused to the b-glucuronidase gene. Transgenic P. patens expressing MpABI1 and its mutant construct, MpABI1-d2, lacking the amino-terminal domain, had reduced freezing and osmotic stress tolerance, and associated with reduced accumulation of ABA-induced late embryogenesis abundant-like boiling-soluble proteins. Furthermore, ABA-induced morphological changes leading to brood cells were not prominent in these transgenic plants. These results suggest that MpABI1 is a negative regulator of ABA signaling, providing unequivocal molecular evidence of PP2C-mediated ABA response mechanisms functioning in liverworts.Land plants are repeatedly challenged by environmental stresses such as desiccation, salinity, and freezing, which can cause irreversible damage to intracellular structures and membranes by severe dehydration. Abscisic acid (ABA), which is known to be a phytohormone responsible for physiological control of seed maturation and germination, bud dormancy, and stomata closure, has been postulated to be involved in the development of cellular dehydration tolerance under stress conditions. In higher plants, water-stress conditions trigger a transient or sustained increase in endogenous ABA content in tissues. Increased ABA stimulates the expression of a number of stress-related genes such as those of late embryogenesis abundant (LEA) proteins, which are thought to protect cells from the damage caused by the dehydration stress (Chandler and Robertson, 1994). It has been demonstrated that various tissue-cultured cells of vascular plants, including those of angiosperms, gymnosperms, and ferns, develop desiccation and freezing tolerance upon exogenous ABA treatment, indicating that ABA plays a key role in stress tolerance at cellular

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Section: Changes In Osmotic and Freezing Stress Tolerance In Transgenmentioning

confidence: 99%

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confidence: 99%

Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

et al. 2010

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“…Similarly, our understanding of the relationship between sexual reproduction in mosses and environmental stress is limited (Convey & Smith, 1993;Stark et al, 2000), despite the fact that mosses are globally prevalent and well known for their ability to tolerate environmental stress (Seel et al, 1992;Lovelock et al, 1995;Meyer & Santarius, 1998;Minami et al, 2003;Oliver et al, 2005;Clarke & Robinson, 2008). The majority of research previously conducted on moss sperm focused on sperm architecture and the composition of the sperm mass (Paolillo, 1977(Paolillo, , 1979(Paolillo, , 1981Bernhard & Renzaglia, 1995;Renzaglia et al, 2001), the mechanism of initial sperm release (Paolillo, 1975), and the behavior of the released sperm mass upon encountering the air-water interface (Muggoch & Walton, 1942;Paolillo, 1981).…”

Section: Introductionmentioning

confidence: 99%

Tolerance to environmental desiccation in moss sperm

2012

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Summary• Sexual reproduction in mosses requires that sperm be released freely into the environment before finding and fertilizing a receptive female. After release from the male plant, moss sperm may experience a range of abiotic stresses; however, few data are available examining stress tolerance of moss sperm and whether there is genetic variation for stress tolerance in this important life stage.• Here, we investigated the effects of environmental desiccation and recovery on the sperm cells of three moss species (Bryum argenteum, Campylopus introflexus, and Ceratodon purpureus).• We found that a fraction of sperm cells were tolerant to environmental desiccation for extended periods (d) and that tolerance did not vary among species. We found that this tolerance occurs irrespective of ambient dehydration conditions, and that the addition of sucrose during dry-down improved cell recovery. Although we observed no interspecific variation, significant variation among individuals within species in sperm cell tolerance to environmental desiccation was observed, suggesting selection could potentially act on this basic reproductive trait.• The observation of desiccation-tolerant sperm in multiple moss species has important implications for understanding bryophyte reproduction, suggesting the presence of a significant, uncharacterized complexity in the ecology of moss mating systems.

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“…In bryophytes, the roles of ABA has been described with respect to their ability to tolerate severe desiccation and freezing environment. [28][29][30][31] Endogenous ABA levels are dramatically increased by dehydration in Exormotheca holstii, Funaria hygrometrica and Riccia fluitans. [29][30][31] In comparison to the studies on desiccation and freezing tolerance, information on the salt tolerance of bryophytes is quite limited.…”

Section: Roles Of Aba In Salt Tolerancementioning

confidence: 99%

Responses and tolerance to salt stress in bryophytes

Wang

Liu

2008

Plant Signaling & Behavior

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During exposure to salt environments, plants could perceive salt signal and transmit the signal to cellular machinery to activate adaptive responses. In bryophytes, salt signal components and transcript factor identified suggest that salt activate adaptive responses to tolerate adverse environments. The ability of bryophytes to tolerate salt is determined by multiple biochemical pathways. Transmembrane transport proteins that mediate ion fluxes play a curial role in ionic and osmotic homeostasis under salt environments. Defense proteins protect cells from denaturation and degradation, as well as from oxidative damage following exposure to salt stress in bryophytes. ABA and salt stress positively affect the expression of common genes that participate in protection plant cells from injure, and ABA may be responsible for the ability to tolerate salt stress in bryophytes. In this paper, we reveal the mechanisms of salt responses and tolerance in bryophytes, and imply conservation between higher plants and bryophytes in response and tolerance to salt stress. IntroductionDue to their sessile nature, plants have to endure adverse environmental conditions such as soil salinity. Excess salts in the soil inhibit plant growth and cause great losses in agricultural productivity worldwide. 1 Therefore, understanding the mechanisms by which plants perceive salt signal and transmit the signal to cellular machinery to active adaptive responses is of fundamental importance to biology. The responses of higher plants to salt stress have been studies intensively at the cellular and molecular levels as well as at the physiological and biochemical levels. 2 However, little is known about the mechanisms underlying salt responses and tolerance in bryophytes.Bryophytes are well known because of their importance in the study of plant systematics and evolution, as well as the tolerance of harsh environments. 3 This review focuses on the responses and tolerance of salt stress in bryophytes. These processes include signal transduction, transcript regulation, ionic and osmotic homeostasis, biosynthesis defense proteins, and the roles of phytohormone in salt stress. Our article will provide the complex mechanisms of salt responses and tolerance in bryophytes. Salt Signal Compoments and Transcript RegulationMolecular and proteomic analyses have shown that several different signal components and transcriptional regulator are involved in salt stress responses. These proteins are phototropin, myelin basic protein kinase, MCamb1 and MCamb2, 14-3-3 like protein and PpDBF1. [4][5][6][7] Phototropin is the blue light receptor that mediates phototropism, chloroplast relocation and stomatal opening in higher plants. 8 In Physcomitrell patens, phototropin and 14-3-3 proteins may work cooperatively to regulate plasma membrane H + -ATPase and maintain ion homeostasis. 4 The 14-3-3 proteins are important eukaryotic regulatory proteins, most of which are involved in cellular signaling pathways. 9 Through regulating the activities of kinases and phosphatase,...

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Abscisic acid-induced freezing tolerance in the mossPhyscomitrella patens is accompanied by increased expression of stress-related genes

Cited by 73 publications

References 38 publications

Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

Tolerance to environmental desiccation in moss sperm

Responses and tolerance to salt stress in bryophytes

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