Abscisic acid in dormant apple seed tissues ‐ a rapid purification scheme using pre‐packed columns and GCMS‐SIM quantitation

Subbaiah, T. K.; Powell, Loyd E.

doi:10.1111/j.1399-3054.1987.tb02868.x

Cited by 13 publications

(12 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same effect was noted simultaneously by Kefeli et al (1969), whereas Subbaiah and Powell (1987) confirmed later the identification of ABA using instrumental methods. The concentration of ABA in the endosperm and seed coat exceeded threefold that in embryo axis and cotyledons.…”

Section: Abscisic Acidsupporting

confidence: 71%

Metabolic control of embryonic dormancy in apple seed: seven decades of research

Lewak

2010

Acta Physiol Plant

View full text Add to dashboard Cite

Embryonic dormancy is defined as a set of blocks imposed upon a process(es) cardinal for growth. In apple seeds, all these blocks are removed as a result of cold treatment (stratification), but some of them are also affected by light and/or hormonal treatments. This review summarizes published data related to the modes of action of above factors on the changes in the levels of endogenous hormones and some other plant growth regulators (e.g., hydrogen cyanide), and on certain enzymes involved in mobilization of seed reserves and catabolism of their hydrolysis products. Phytochrome and activities of acid lipase and a protease have been indicated as receptors of light and low temperature, respectively. Several chains of events initiated by these two environmental factors and leading to dormancy removal are proposed, and the sites of their control by hormones and HCN are indicated. These chains are postulated to contribute to the elimination of particular blocks that hinder germination and therefore to be involved in the mechanisms of dormancy breakage.

show abstract

Section: Abscisic Acidsupporting

confidence: 71%

Metabolic control of embryonic dormancy in apple seed: seven decades of research

Lewak

2010

Acta Physiol Plant

View full text Add to dashboard Cite

show abstract

“…Frequently used labor-intensive procedures have required a lengthy sequence of steps including solvent partitioning, open column chromatography, and HPLC (5,20). Recent procedures have been greatly simplified by using newly available preparative minicolumns and chromatographic cartridges that reduce the time required for sample preparation, particularly in GC-SIM-MS' analysis (1,18).…”

Section: Methodsmentioning

confidence: 99%

A Simple Purification of Indole-3-Acetic Acid and Abscisic Acid for GC-SIM-MS Analysis by Microfiltration of Aqueous Samples through Nylon

Dunlap

Guinn²

1989

Plant Physiol.

View full text Add to dashboard Cite

A simple procedure was developed for the partial purification of plant tissue samples to be analyzed simultaneously for indole-3-acetic acid (IAA) and abscisic acid (ABA). The procedure relies on removal of contaminants by filtration through nylon and partitioning into dichloromethane. This procedure successfully purified both IAA and ABA from muskmelon, cotton, and broccoli tissue. Twenty individual samples can be purified and methylated in 8 h for analysis of free IAA and ABA with gas chromatographyselected ion monitoring-mass spectrometry. The use of microfiltration of aqueous samples through nylon offers new opportunities for improving the efficiency of existing sample purification procedures.with molecular fragments unique to the hormone of interest are monitored (3,15,16). The high degree of detector selectivity coupled with the efficiency of capillary gas chromatography requires less sample purity than normally required for analysis of IAA and ABA from plant tissue.Nylon preparations, including nylon 66, have been used to inactivate and remove phenolic compounds from plant samples (7, 9). Microfiltration through nylon 66 syringe filters was recently reported to be an effective step in the preparation of plant samples for the immunoassay of ABA (13, 21). Consequently, experiments were conducted to assess the potential value of using nylon 66 microfiltration in the preparation of plant samples for the analysis of IAA and ABA by GC-SIM-MS. IAA and ABA (4,8,12,17). The GC-MS analysis is particularly sensitive when only a few selected ions associated 'Abbreviations: GC-SIM-MS, gas chromatography-selected ion monitoring-mass spectrometry; BHT, butylated hydroxytoluene; MATERIALS AND METHODSMeABA, methyl ester of ABA; MeIAA, methyl ester of IAA. Sample PreparationCotton floral buds (squares) and fruiting branches were harvested from field plots prior to anthesis. Muskmelon flowers were harvested from greenhouse-grown plants at anthesis. Broccoli heads including floral buds were harvested at commercial maturity from field plots. Each plant tissue was lyophilized, homogenized with a Polytron PT 10/35 (Brinkman Instruments, Westbury, NY)2 in 70% acetone (40 mL g-' dry weight that contained 200 mg of BHT and 100 mg of Na ascorbate L-' (6).

show abstract

“…Only the free cis-trans ABA was determined in these experiments. Details of the method are given in Subbaiah and Powell (14). The treatments that received red light were illuminated for 5 min, followed by a 30 min period before the tissues were frozen for lyophilization.…”

Section: Methodsmentioning

confidence: 99%

“…Measurements of ABA content of root caps (1 mm tip) and elongating zones (1-5 mm from tip) were made by Dr. Thilak Subbaiah, using GC-MS-selected ion monitoring as described in Subbaiah and Powell (14). The was dried, and methylated with ethereal diazomethane.…”

Section: Methodsmentioning

confidence: 99%

Interactions between Red Light, Abscisic Acid, and Calcium in Gravitropism

Leopold¹,

LaFavre²

1989

Plant Physiol.

View full text Add to dashboard Cite

The effect of red light on orthogravitropism of Merit com (Zea mays L.) roots has been attributed to its effects on the transduction phase of gravitropism (AC Leopold, SH Wettlaufer [1988] Plant Physiol 87:803-805). In an effort to characterize the orthogravitropic transduction system, comparative experiments have been carried out on the effects of red light, calcium, and abscisic acid (ABA). The red light effect can be completely satisfied with added ABA (100 micromolar) or with osmotic shock, which is presumed to increase endogenous ABA. The decay of the red light effect is closely paralleled by the decay of the ABA effect. ABA and exogenous calcium show strong additive effects when applied to either Merit or a line of com which does not require red light for orthogravitropism. Measurements of the ABA content show marked increases in endogenous ABA in the growing region of the roots after red light. The interpretation is offered that red light or ABA may serve to increase the cytoplasmic concentrations of calcium, and that this may be an integral part of orthogravitropic transduction.

show abstract

Abscisic acid in dormant apple seed tissues ‐ a rapid purification scheme using pre‐packed columns and GCMS‐SIM quantitation

Cited by 13 publications

References 8 publications

Metabolic control of embryonic dormancy in apple seed: seven decades of research

Metabolic control of embryonic dormancy in apple seed: seven decades of research

A Simple Purification of Indole-3-Acetic Acid and Abscisic Acid for GC-SIM-MS Analysis by Microfiltration of Aqueous Samples through Nylon

Interactions between Red Light, Abscisic Acid, and Calcium in Gravitropism

Contact Info

Product

Resources

About