Abscisic Acid Biosynthesis and Catabolism

Nambara, Eiji; Marion‐Poll, Annie

doi:10.1146/annurev.arplant.56.032604.144046

Cited by 1,858 publications

(1,624 citation statements)

References 144 publications

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“…Thus, ABA 8Ј-hydroxylation is the major ABA catabolic pathway in tomato ovaries (our work) as was suggested for Arabidopsis (Kushiro et al 2004). ABA can be transported and stored as ABA-GE (Nambara and Marion-Poll 2005) and the high levels in unpollinated ovaries could mean that ABA-GE is transported to and stored in the ovary. Altogether, these data strengthen our hypothesis that after pollination ABA levels are reduced mainly through ABA 8Ј-hydroxylation.…”

Section: Regulation Of Aba Biosynthesis and Catabolismsupporting

confidence: 64%

“…In plant cells, ABA is mainly inactivated by 8Ј-hydroxylation (Nambara and Marion-Poll 2005), which is in Arabidopsis catalyzed by four cytochrome P450 mono-oxygenases, AtCYP707A1 to AtCYP707A4 (Kushiro et al 2004). We previously identiWed a tomato homolog of AtCYP707A4, which was strongly induced in the ovary after pollination (Vriezen et al 2008).…”

Section: Regulation Of Aba Catabolismmentioning

confidence: 99%

“…PA levels did not change, but DPA levels increased from 0.44 to 1.20 ng/mg DW in ovaries after pollination. 7Ј-and 9Ј-ABA hydroxylation can also inactivate ABA in several plant species (Nambara and Marion-Poll 2005). The product of ABA 9Ј-hydroxylation is 9Ј-hydroxy ABA which is converted to neo-phaseic acid (neo-PA).…”

Section: Regulation Of Aba Catabolismmentioning

confidence: 99%

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Abscisic acid levels in tomato ovaries are regulated by LeNCED1 and SlCYP707A1

Nitsch

Oplaat

Feron

et al. 2009

Planta

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Although the hormones, gibberellin and auxin, are known to play a role in the initiation of fruits, no such function has yet been demonstrated for abscisic acid (ABA). However, ABA signaling and ABA responses are high in tomato (Solanum lycopersicum L.) ovaries before pollination and decrease thereafter (Vriezen et al. in New Phytol 177:60-76, 2008). As a Wrst step to understanding the role of ABA in ovary development and fruit set in tomato, we analyzed ABA content and the expression of genes involved in its metabolism in relation to pollination. We show that ABA levels are relatively high in mature ovaries and decrease directly after pollination, while an increase in the ABA metabolite dihydrophaseic acid was measured. An important regulator of ABA biosynthesis in tomato is 9-cis-epoxy-carotenoid dioxygenase (LeNCED1), whose mRNA level in ovaries is reduced after pollination. The increased catabolism is likely caused by strong induction of one of four newly identiWed putative (+)ABA 8Ј-hydroxylase genes. This gene was named SlCYP707A1 and is expressed speciWcally in ovules and placenta. Transgenic plants, overexpressing SlCYP707A1, have reduced ABA levels and exhibit ABA-deWcient phenotypes suggesting that this gene encodes a functional ABA 8Ј-hydroxylase. Gibberellin and auxin application have diVerent eVects on the LeNCED1 and SlCYP707A1 gene expression. The crosstalk between auxins, gibberellins and ABA during fruit set is discussed.

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Section: Regulation Of Aba Biosynthesis and Catabolismsupporting

confidence: 64%

Section: Regulation Of Aba Catabolismmentioning

confidence: 99%

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Abscisic acid levels in tomato ovaries are regulated by LeNCED1 and SlCYP707A1

Nitsch

Oplaat

Feron

et al. 2009

Planta

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“…Epoxidation of zeaxanthin leads to the formation of violaxanthin that can be converted into neoxanthin, the end product of the branch in the plastid. Finally, cleavage of violaxanthin and neoxanthin, followed by further modifications, produces ABA in the cytoplasm [21].…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis Spontaneous Cell Death1 gene, encoding a ζ-carotene desaturase essential for carotenoid biosynthesis, is involved in chloroplast development, photoprotection and retrograde signalling

et al. 2007

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Carotenoids, a class of natural pigments found in all photosynthetic organisms, are involved in a variety of physiological processes, including coloration, photoprotection, biosynthesis of abscisic acid (ABA) and chloroplast biogenesis. Although carotenoid biosynthesis has been well studied biochemically, the genetic basis of the pathway is not well understood. Here, we report the characterization of two allelic Arabidopsis mutants, spontaneous cell death1-1 (spc1-1) and spc1-2. The weak allele spc1-1 mutant showed characteristics of bleached leaves, accumulation of superoxide and mosaic cell death. The strong mutant allele spc1-2 caused a complete arrest of plant growth and development shortly after germination, leading to a seedling-lethal phenotype. Genetic and molecular analyses indicated that SPC1 encodes a putative z-carotene desaturase (ZDS) in the carotenoid biosynthesis pathway. Analysis of carotenoids revealed that several major carotenoid compounds downstream of SPC1/ZDS were substantially reduced in spc1-1, suggesting that SPC1 is a functional ZDS. Consistent with the downregulated expression of CAO and PORB, the chlorophyll content was decreased in spc1-1 plants. In addition, expression of Lhcb1.1, Lhcb1.4 and RbcS was absent in spc1-2, suggesting the possible involvement of carotenoids in the plastid-to-nucleus retrograde signaling. The spc1-1 mutant also displays an ABA-deficient phenotype that can be partially rescued by the externally supplied phytohormone. These results suggest that SPC1/ZDS is essential for biosynthesis of carotenoids and plays a crucial role in plant growth and development.

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“…Chemically, ABA (C 15 H 20 O 4 ) is a sesquiterpenoid derived from isopentenyl pyrophosphate synthesized in plastids through the 2C-methyl-D-erythritol-4-phosphate (MEP) pathway [2]. ABA contains one asymmetric carbon atom at C1´, the natural form is S(+)ABA and the side chain of the molecule is present in the 2-cis,4-trans isomeric state (Fig.…”

Section: Introductionmentioning

confidence: 99%

Structural insights into PYR/PYL/RCAR ABA receptors and PP2Cs

et al. 2012

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Abscisic acid (ABA) plays an essential function in plant physiology since it is required for biotic and abiotic stress responses as well as control of plant growth and development. A new family of soluble ABA receptors, named PYR/PYL/RCAR, has emerged as ABA sensors able to inhibit the activity of specific protein phosphatases type-2C (PP2Cs) in an ABAdependent manner. The structural and functional mechanism by which ABA is perceived by these receptors and consequently leads to inhibition of the PP2Cs has been recently elucidated. The module PYR/PYL/RCAR-ABA-PP2C offers an elegant and unprecedented mechanism to control phosphorylation signaling cascades in a ligand-dependent manner. The knowledge of their three-dimensional structures paves the way to the design of ABA agonists able to modulate the plant stress response.

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Abscisic Acid Biosynthesis and Catabolism

Cited by 1,858 publications

References 144 publications

Abscisic acid levels in tomato ovaries are regulated by LeNCED1 and SlCYP707A1

Abscisic acid levels in tomato ovaries are regulated by LeNCED1 and SlCYP707A1

The Arabidopsis Spontaneous Cell Death1 gene, encoding a ζ-carotene desaturase essential for carotenoid biosynthesis, is involved in chloroplast development, photoprotection and retrograde signalling

Structural insights into PYR/PYL/RCAR ABA receptors and PP2Cs

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