Abscisic acid and 3-indolacetic acid levels during the reinvigoration process of Pinus radiata D. Don adult material

Abstract

The maturation and phase change processes in woody species have not been completely clarified, specially the role of growth regulators during the maturation phase. Understanding their role better will allow to comprehend the physiological aspects of the species, which would allow to chose the optimal management conditions in forestry programs and make them more useful. In the present study, the endogenous content of abscisic acid (ABA) and 3-indolacetic acid (IAA) were quantified by high performance liquid chromatography, in the reinvigoration process of elite Pinus radiata material, during the development of homomicrografts (P. radiata/P. radiata) and heteromicrografts (P. radiata/Pinus caribaea), developed under in vitro conditions. The results showed an increase in the endogenous content of ABA through time and a decrease in the content of IAA. Significant differences were found in the endogenous content of ABA, where the maximum values were detected at 120 days of micrograft culture, being similar to the values obtained in juvenile buds. As well, significant differences were also detected in the endogenous content of IAA in the micrografted buds during all the development phases, compared with adult buds. However, no significant differences were detected in the endogenous content of ABA and IAA, regarding the type of rootstock employed. Considering the endogenous content of ABA and IAA, the micrografted plant material would be equal to the juvenile buds, since statistically they were equal to these buds, considering that the values obtained in this case are independent from the type of rootstock used.

Topophysis in Corymbia torelliana × C. citriodora seedlings: adventitious rooting capacity, stem anatomy, and auxin and abscisic acid concentrations

“…Another hormone, abscisic acid (ABA), regulates primary root elongation and lateral root formation (Chen et al 2006;Yamaguchi and Sharp 2010) and influences the biosynthesis and activity of IAA (Mwange et al 2003). ABA and IAA may interact in regulating rooting potential, root growth and lateral root development (Materán et al 2009;Shkolnik-Inbar and Bar-Zvi 2010;Yamaguchi and Sharp 2010). Rooting capacity is positively correlated with IAA concentration, but negatively correlated with ABA concentration, in grapevine cuttings (Kelen and Ozkan 2003).…”

Photochemical Efficiency during the Establishment and Consolidation Phases of in Vitro Pinus radiata Micrograft Made from Scions of Different Ontogenetic Age

“…Otherwise, the concentrations of growth regulators in the shoot tips vary depending on their position in the ortet. [37] Reported higher concentration of auxins and cytokinins in apical shoot tips than basal ones, which may improve tissue responses to the in vitro introduction, specifically during P. radiata micrograft establishment [38].…”

Reverse-cleft in vitro micrografting of Ziziphus jujuba Mill. Infected with jujube witches’ broom (JWB)

“…Open ring of silicone are commonly used to maintain the contact between scion and rootstock in micrografting (Angeles et al 1996;Materán et al 2009). In our study, aluminum foil was used as an alternation.…”