Abscisic acid (ABA)-mediated inhibition of seed germination involves a positive feedback regulation of ABA biosynthesis in Arachis hypogaea L.

Hu, Bo; Wan#, Xiao-rong; Liu, Xiaohui; Guo, Dongliang; Li, Ling

doi:10.5897/ajb10.1819

Cited by 15 publications

(3 citation statements)

References 42 publications

(55 reference statements)

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“…Only lowering the auxin concentration (that is, removing the ‘on’ switch) does not reduce other phytohormones. Considering this hypothesis, a second ‘on’ switch may be required to accurately determine the time frame for cell enlargement [ 25 – 30 ]. It was found that the content of ABA is different in embryogenic cell clusters, globular embryos, torpedo embryos, and cotyledon embryos [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Insights into the correlation between Physiological changes in and seed development of tartary buckwheat (Fagopyrum tataricum Gaertn.)

Liu

Zheng

et al. 2018

BMC Genomics

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BackgroundTartary buckwheat (Fagopyrum tataricum Gaertn.) is a widely cultivated medicinal and edible crop with excellent economic and nutritional value. The development of tartary buckwheat seeds is a very complex process involving many expression-dependent physiological changes and regulation of a large number of genes and phytohormones. In recent years, the gene regulatory network governing the physiological changes occurring during seed development have received little attention.ResultsHere, we characterized the seed development of tartary buckwheat using light and electron microscopy and measured phytohormone and nutrient accumulation by using high performance liquid chromatography (HPLC) and by profiling the expression of key genes using RNA sequencing with the support of the tartary buckwheat genome. We first divided the development of tartary buckwheat seed into five stages that include complex changes in development, morphology, physiology and phytohormone levels. At the same time, the contents of phytohormones (gibberellin, indole-3-acetic acid, abscisic acid, and zeatin) and nutrients (rutin, starch, total proteins and soluble sugars) at five stages were determined, and their accumulation patterns in the development of tartary buckwheat seeds were analyzed. Second, gene expression patterns of tartary buckwheat samples were compared during three seed developmental stages (13, 19, and 25 days postanthesis, DPA), and 9 765 differentially expressed genes (DEGs) were identified. We analyzed the overlapping DEGs in different sample combinations and measured 665 DEGs in the three samples. Furthermore, expression patterns of DEGs related to phytohormones, flavonoids, starch, and storage proteins were analyzed. Third, we noted the correlation between the trait (physiological changes, nutrient changes) and metabolites during seed development, and discussed the key genes that might be involved in the synthesis and degradation of each of them.ConclusionWe provided abundant genomic resources for tartary buckwheat and Polygonaceae communities and revealed novel molecular insights into the correlations between the physiological changes and seed development of tartary buckwheat.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5036-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Insights into the correlation between Physiological changes in and seed development of tartary buckwheat (Fagopyrum tataricum Gaertn.)

Liu

Zheng

et al. 2018

BMC Genomics

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“…Overexpression of LeNCED1 in Lycopersicon esculentum delays seed germination (Thompson, et al 2000). AhNCED2 plays a positive role in maintaining seed dormancy in Arachis hypogaea (Bo, et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Functional Identification of 9-Cis-Epoxycarotenoid Dioxygenase Genes in Double Dormant Plant-Herbaceous Peony

Fei

Duan

et al. 2022

Preprint

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Seed dormancy and germination is a complex process, which is affected by external environmental conditions and internal factors independently or mutually. Phytohormones play an important regulatory role in this process. ABA was the main phytohormone affecting herbaceous peony seed dormancy release. However, the mechanism of ABA in the dormancy release of herbaceous peony needs to be further explored. Here, transcriptome data was screened from the perspective of ABA metabolism, and significantly differentially expressed PlNCED1 and PlNCED2 were obtained. We found that their expression trends were positively correlated with ABA content. Among them, PlNCED2 had a stronger regulatory effect on ABA content and was more sensitive to exogenous ABA. Overexpression and silencing of PlNCEDs in callus could affect the expression of PlCYP707As and the content of endogenous ABA. Through the observation of seed germination of Arabidopsis thaliana (A. thaliana), we found PlNCED1 and PlNCED2 promoted seed dormancy, and the promotion effect of PlNCED2 was more obvious. In general, PlNCED1 and PlNCED2 participated in the dormancy release of herbaceous peony seeds by regulating the accumulation of endogenous ABA. Our work can reveal the molecular mechanism and related theories of ABA involved in herbaceous peony seed dormancy release.

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“…Short and shallow dormancy may result in the harmful phenomenon-of pre-harvest sprouting, in which the seeds gain the ability to germinate while they are still on the mother plant (Kermode, 2004). The process triggers in the final stage of maturation of the seed, at the end of the cell cycle and is controlled by several genes and environmental factors, with broad hormonal influence (Hu et al, 2010;Footitt et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Research Article Differential expression of dormancy-Associated genes in fastigiata and hypogaea peanut

Silva¹,

Silva²,

Lima³

et al. 2017

Genet. Mol. Res.

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Seed dormancy is a temporary replacement state observed in some higher plants, which prevents germination under unfavorable conditions. In several species, dormancy process is triggered by hormonal factors, initiated by ABA and precursor enzymes. In this work, we investigated the relationship between dormancy and the expression of ARP, DMR1, and NCED genes in upright and runner peanut seeds. Eight dormancy-contrasting genotypes were previously phenotyped based on germination traits, during three storage periods. Expression of ARP, DMR1 and NCED transcripts was analyzed by qRT-PCR, from endosperm and embryo tissues. Higher expression of NCED and ARP were found in embryo tissues from runner genotypes, IAC Caiapo and LGoPE-06, confirming findings in literature as to association of these genes with seed dormancy. Higher expression of DMR1 was found only in endosperm of upright materials (L7 Bege and BR1). We suggest the NCED is a functional molecular marker to identify dormancy in Lima LM et al 2 Genetics and Molecular Research 16 (4): gmr16039820 seeds, contributing to selection procedures of runner genotypes in peanut breeding.

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Abscisic acid (ABA)-mediated inhibition of seed germination involves a positive feedback regulation of ABA biosynthesis in Arachis hypogaea L.

Cited by 15 publications

References 42 publications

Insights into the correlation between Physiological changes in and seed development of tartary buckwheat (Fagopyrum tataricum Gaertn.)

Insights into the correlation between Physiological changes in and seed development of tartary buckwheat (Fagopyrum tataricum Gaertn.)

Functional Identification of 9-Cis-Epoxycarotenoid Dioxygenase Genes in Double Dormant Plant-Herbaceous Peony

Research Article Differential expression of dormancy-Associated genes in fastigiata and hypogaea peanut

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