Abortive bacteriophage T4 head assembly in mutants of Escherichia coli

Coppo, Anna; Manzi, Andrea; Pulitzer, John F.; Takahashi, Hideo

doi:10.1016/0022-2836(73)90081-8

Cited by 126 publications

(52 citation statements)

References 33 publications

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“…We have chosen the tubular forms -i.e., polyheads from mutants in genes 20,22,24,40, and P H I and capsids of giant phages (8) -as provisional representatives of the two groups for our studies of subunit arrangement by optical diffraction. Among these tubular forms are those arising simultaneously with 7-particles in mutants in gene 24; these polyheads seem t o grow out of -or are capped by -a 7-particle (1).…”

Section: Introductionmentioning

confidence: 99%

The transformation of τ particles into t4 heads. II. Transformations of the surface lattice an related observations on form determination

et al. 1974

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In part 1 of this paper ( I ) we give evidence that the P23-capsoid of 7-particles is transformed in situ i n t o the P23*-capsid of' normal phage. Using t h e polymorphism of phage T4, we have chosen polyheads as representative of P 2 3 assemblies and giant phages as representative of P23* assemblies in order t o study their surface crystals by optical filtration of micrographs. We found for polyheads a lattice constant of 1 I 2 a with the typical hexameric, ringlike capsomer and for t h e giants a lattice constant of 124 a with quite a different capsomer morphology, of t h e t y p e (h+l). From the stoichiometry of t h e proteins composing the normal capsid we conclude that the prototner is a single P23* molecule and that the minor capsidproteins must be in singular positions on t h e surface lattice o r on t h e polyhedral head (center of capsomers, vertices, o r basal part).We extrapolate the findings on the giant head to t h e normal head and give a geometric model which is consistent with 1 , I 00 molecules o f P23* per capsid.We discuss t h e part of form inheritance contrihuted by P23 and t h e other formgiving gene products and give evidence that morphologic characters are t h e result of pairs of a reaction chain of interacting gene products. The example we give is the giant head produced by a t s mutant in gene 2 4 at 3h'C.

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Section: Introductionmentioning

confidence: 99%

The transformation of τ particles into t4 heads. II. Transformations of the surface lattice an related observations on form determination

et al. 1974

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“…The growth of phages might be prevented by the defect of maturation caused by the anomaly of the membrane. Several mutants of E. toll which suppressed the multiplication of phages T4 and lambda have been isolated so far (12)(13)(14)(15). The following results suggested that a single mutation affected both the cell division and phage multiplication in the case of E. toll strain ts612.…”

Section: Discussionmentioning

confidence: 91%

A Thermosensitive Mutant of Escherichia Coli With a Defect in the Process of Cell Division

Masamune

1975

J. Gen. Appl. Microbiol.

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A thermosensitive mutant of Escherichia coli (E. coli strain ts612) was isolated by the transduction of polA+ as a selective marker with phage Plc which had been mutagenized with hydroxylamine. The mutation was mapped between ilv (74.7 min) and metE (75.5 min). When the incubation temperature of this mutant was shifted from 30° to 42°, the cell division was interrupted immediately after the shift of temperature, although the optical density at 660 nm of the culture continued to increase for 90 min. The mutant did not support the multiplications of phages T4 and lambda at 42°, while it allowed the growth of phage T7. Electrophoretic analysis of membrane proteins of the mutant suggested that a part of the membrane protein was diminished when the mutant was incubated at the restrictive temperature.Specialized mutations in Escherichia coli can be induced by means of transduction with mutagenized phage P1. When an appropriate genetic marker was transduced with mutagenized phage particles, the transductants would involve a mutant which had a mutation closely linked to the selected marker. By this means it is possible to induce mutation in the specific region of the chromosome. Several mutants of Salmonella typhimurium were isolated by a similar method using a mutagenized phage P22 (1).With the intention of obtaining a new class of mutants having defects (2, 3) in the machinery of DNA synthesis, transduction of E. coli polA was made with the mutagenized phage Plc. Several thermosensitive mutants were isolated among the polA+ transductants and were characterized. Since one of these thermosensitive mutants seemed to have various interesting properties concerning the cell division and phage growth, the characterization of the mutant was performed which is described in this report.

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“…The discovery of the E. coli genes encoding the GroEL chaperone machine was made in the early 1970s, again through the efforts of bacteriophage geneticists isolating gro-type bacterial mutants that blocked either phage l (Georgopoulos et al 1972(Georgopoulos et al , 1973Sternberg 1973) or phage T4 (Takano and Kakefuda 1972;Coppo et al 1973;Revel et al 1980). My studies on groE at Stanford were carried out in collaboration with a fellow postdoc, Roger Hendrix.…”

Section: The Discovery Of the Groel/groes Chaperone Machinementioning

confidence: 99%

Toothpicks, Serendipity and the Emergence of the Escherichia coli DnaK (Hsp70) and GroEL (Hsp60) Chaperone Machines

Georgopoulos

2006

Genetics

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Abortive bacteriophage T4 head assembly in mutants of Escherichia coli

Cited by 126 publications

References 33 publications

The transformation of τ particles into t4 heads. II. Transformations of the surface lattice an related observations on form determination

The transformation of τ particles into t4 heads. II. Transformations of the surface lattice an related observations on form determination

A Thermosensitive Mutant of Escherichia Coli With a Defect in the Process of Cell Division

Toothpicks, Serendipity and the Emergence of the Escherichia coli DnaK (Hsp70) and GroEL (Hsp60) Chaperone Machines

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