Abortifacient potential of methanolic extract of Anthocephalus cadamba stem bark in mice

Shaikh, Muhammad Vaseem; Kala, Manika; Ravat, Nirav M; Nivsarkar, Manish

doi:10.1016/j.jep.2015.07.031

Cited by 9 publications

(8 citation statements)

References 21 publications

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“…Isolated natural products (1-10) belong to different classes such as steroids (1 and 6), terpenoids (2, 4 and 5), alkaloids (7 and 8), irridoid glucoside (9) and phenolic compounds (3 and 10) (Figure 1) and were characterized using 1 HNMR, 13 CNMR, IR and HRMS data as β-sitosterol (1), [20] ursolic acid (2), [21] vanillic (7), cadambine tetraacetate (7a), 3β-dihydrocadambine (8), 3β-dihydrocadambinepentaacetate (8a), 7-O-acetyl loganin (9), and hexyl p-coumarate (10).…”

Section: Resultsmentioning

confidence: 99%

“…The concentrated DCM : MeOH (1 : 1) extract was fractionated sequentially with petroleum ether, chloroform and methanol by liquid-liquid partition chromatography to yield three respective fractions. The chloroform, petroleum ether and aqueous methanolic fractions were separately subjected to column chromatography over silica gel to afford ten compounds (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). Four acetylated products (2 a,5 a,7 aand8 a) obtained through acetylation of 2, 5, 7 and 8 respectively.…”

Section: Resultsmentioning

confidence: 99%

“…[6] Ayurvedic Pharmacopoeia of India described the use of stem bark in burning sensations, female genital disorders, ulcers and hemorrhagic diseases. [7] A variety of stembark extracts have been prepared by various researchers and reported their different biological activities such as methanolic extract of A. cadamba stembark has been reported to possess anticancer activity in Ehrlich ascites carcinoma (EAC) cells treated mice [8] and in vitro and in vivo anti-inflammatory activity in LPSactivated murine macrophage RAW 264.7 cellline and carragenan induced paw edema in wistar albino rats, [9] ethanolic extract of bark possess sedative and anti-epileptic effects in animal models [10] and ethylacetate and n-butanol fractions of stem bark showed promising anti-oxidant activity whereas the n-butanol fraction inhibited the growth of colon cancer cells with GI 50 value 54.36 μg/ml. [11] Further, Anthocephalus cadambais a rich source of diverse secondary metabolites having broad range of biological activities.…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Cytotoxicity Evaluation, Docking, ADMET and Drug Likeness Studies of Secondary Metabolites from the Stem Bark ofAnthocephalus cadamba(Roxb.)

et al. 2022

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Phytochemical investigation of the stem bark of Anthocephalus cadamba(Rubiaceae) led to the isolation of total ten secondary metabolites (1‐10) including β–sitosterol (1), ursolic acid (2), vanillic acid (3), quinovic acid (4), 3‐O‐[α‐L‐rhamnopyranosyl]‐quinovic acid (5), β‐sitosterol β‐D‐glucoside (6), cadambine (7), 3β‐dihydrocadambine (8), 7‐O‐acetyl loganin (9) and hexyl p‐coumarate (10). Four acetylated derivatives 2 a,5 a,7 aand8 aobtained through acetylation of compounds 2, 5, 7 and 8 respectively. All the compounds characterized using 1H and 13CNMR, HRESIMS and IR and comparison with literature. Compound 4showed the best cytotoxic activity with IC50 value 4 and 7 μM against pancreatic (MIA‐pa‐Ca‐2) and leukemia (HL‐60) cancer cells respectively. Docking results supported the anticancer potential of compound 4via inhibition of EGFR receptor (PDB ID:3POZ) and its pharmacokinetic profile found optimized as per the Lipinski's filter. Therefore compound 4has the potential to be developed as an anticancer agent against pancreatic cancer cells.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation, Cytotoxicity Evaluation, Docking, ADMET and Drug Likeness Studies of Secondary Metabolites from the Stem Bark ofAnthocephalus cadamba(Roxb.)

et al. 2022

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“…The extract at concentrations of 50mg/kg and 200mg/kg caused disorganization of the arrangement of the uterine epithelium which is observed as one of the mechanisms described in abortifacient activities of a plant on the uterus. Other mechanisms of abortion could possibly be observed through changes in the uterine mileu, altered hormone levels, luteolysis and estrogenicity (19,20).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Khaya senegalensis on the Endometrial and Vaginal Epithelium: A Histological and Morphometrical Study

Dani¹,

Attah²,

Dibal³

2022

IJAM

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Introduction: Khaya senegalensis is a plant with many medicinal uses and application which could serve as an available and alternative source of medication. It has been used traditionally as an abortifacient agent following folkloric information handed down by word mouth. The aim of this study is to validate this claim by investigating the effect of the extract on the endometrial and vaginal epithelium. Methodology: Four groups of albino rats were administered different dosages of the extract orally and after 28 days, the rats were sacrificed. The micrographs of the uterus and vagina were studied for histologically observations. Morphometric analysis was also carried out to determine the thickness of the epithelial lining in all groups. Measurements were analyzed by one-way ANOVA followed by Dunnett’s multiple comparisons test using GraphPad Prism. Results: Histological observations showed that the extract at a concentration of 50mg/kg and 200mg/kg disrupted the epithelial lining and as the concentrations administered increased, surface and stromal endometrial glands became smaller and sparse and the endometrial stroma became less compact. Morphometric analysis revealed a significant increase of endometrial epithelium in the group administered the least concentration of the extract. Vaginal epithelial thickness was also significantly reduced in all groups. Conclusion: Results obtained suggests that the extract has an anti-fertility effect on the lining of the uterus and vagina. Further studies are recommended.

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“…Pharmacological Studies of Herbal Drugs on Female Reproduction : Abortifacient potential of methanolic extract of Anthocephalus cadamba stem bark was demonstrated in mice. The administration of extract in pregnant mice reduced the number of live fetus, weight and survival ratio of the fetus, number of corpora lutea (CL), progesterone, estradiol and LH whereas the number of dead fetus, number of mice that aborted, percentage vaginal opening and post-implantation loss increased significantly (Shaikh et al, 2015). Methanolic extract of Thevetia peruviana leaves containing quercetin 0.0326% and kaempferol 0.138% exhibited a significant antifertility potential by decreasing the progesterone level (Samanta et al, 2016).…”

Section: Female Reproductive Systemmentioning

confidence: 99%

Endocrine Pharmacology Research in India: Scientific Progress in Last Five Years

Srinivasan¹,

Sharma²

2018

Proceedings of the Indian National Science Academy

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During the last five years, significant scientific effort has been made in the field of endocrine pharmacology by Indian researchers. Endocrine disorders like diabetes and disorders of the reproductive systems and melatonin are the major areas of research interest of the Indian scientists. Though the researchers have investigated both natural products and synthetic compounds, the reported studies have focused primarily on plant extracts to substantiate their use scientifically based on their folklore use. Diabetes is fast gaining the status of a potential epidemic in India with approximately 69 million people in the world. Various pharmacological investigations on herbal and synthetic compounds have been carried out for the treatment of diabetes and its complications. Saroglitazar, a novel PPARα/γ agonist is the first indigenously developed synthetic antidiabetic drug by an Indian pharmaceutical company as approved for the treatment of type 2 diabetes in June 2013. The potential of certain cell based therapies and nanoformulations were also investigated for different diabetes treatment. The scientific rationale for the use of various herbs on male and female reproduction with respect to aphrodiasic, fertility and antifertility properties has been explored. In addition to role of melatonin in chronobiology, it has been reported to possess multiple pharmacological actions such as antioxidant, anti-inflammatory and neuroprotective actions. Only few Indian scientists have studied on endocrine pharmacology related to adrenal, tyroid and parathyroid gland disorders. In addition, the effects of some endocrine disruptors, toxic chemicals and heavy metals on endocrine systems were also studied. In this review, we have compiled the research articles on scientific progress made in the field of endocrine pharmacology from India between years 2012-2017.

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Abortifacient potential of methanolic extract of Anthocephalus cadamba stem bark in mice

Cited by 9 publications

References 21 publications

Isolation, Cytotoxicity Evaluation, Docking, ADMET and Drug Likeness Studies of Secondary Metabolites from the Stem Bark ofAnthocephalus cadamba(Roxb.)

Isolation, Cytotoxicity Evaluation, Docking, ADMET and Drug Likeness Studies of Secondary Metabolites from the Stem Bark ofAnthocephalus cadamba(Roxb.)

The Effect of Khaya senegalensis on the Endometrial and Vaginal Epithelium: A Histological and Morphometrical Study

Endocrine Pharmacology Research in India: Scientific Progress in Last Five Years

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