ABO-mismatched marrow processing for transplantation: Comparative results of 80 procedures performed with Cobe Spectra and Spectra Optia

Red blood cell depletion in small‐volume bone marrow processing using manipulation with third‐party red blood cells: A comparison of the performance of the COBE spectra and the spectra Optia systems

“…Moreover, we observed higher RBC removal efficiency and lower post‐process RBC volume in Spectra Optia than in COBE Spectra when processing low‐volume bone marrow samples, while the recovery rates of TNC and MNC were comparable. These findings are consistent with a previous study that reported that large bone marrow volume processing with Spectra Optia had advantages over that with COBE Spectra in terms of RBC depletion efficiency 23 . We also revealed that third‐party RBC manipulation for low RBC volume bone marrow processing did not adversely affect TNC and MNC recoveries in either apheresis device.…”

“…These findings are consistent with a previous study that reported that large bone marrow volume processing with Spectra Optia had advantages over that with COBE Spectra in terms of RBC depletion efficiency. 23 We also revealed that third-party RBC manipulation for low RBC volume bone marrow processing did not adversely affect TNC and MNC recoveries in either apheresis device. In addition, Spectra Optia achieved similar CD34 + cell recovery to that in large bone marrow volume processing using the same device.…”

“…Different manual or semiautomated techniques are used for RBC depletion before ABO‐incompatible transplantation or cryopreservation; these include gravity sedimentation with hydroxyethyl starch (HES) 7–9 or dextran, 10 the addition of third‐party RBCs to buffy coat, 11,12 and Ficoll‐Hypaque used together with blood cell separators 2,13–15 . Bone marrow processing using cell separation devices has recently been developed 16–23 . COBE Spectra (COBE BCT, Lakewood, CO, USA) is a continuous flow cell separator that isolates cells through centrifugation and buffy layer formation under the manual interface management.…”

“…2,[13][14][15] Bone marrow processing using cell separation devices has recently been developed. [16][17][18][19][20][21][22][23] COBE Spectra (COBE BCT, Lakewood, CO, USA) is a continuous flow cell separator that isolates cells through centrifugation and buffy layer formation under the manual interface management. Spectra Optia (Terumo BCT, Lakewood, CO, USA) provides cell separation through continuous flow centrifugation and an automated interface management algorithm.…”

“…These apheresis devices are used to effectively remove RBCs from large bone marrow volumes with a high rate of hematopoietic cell subpopulation recovery. [18][19][20][21][22][23] However, low RBC volume is an obstacle to the processing of small-volume bone marrow collected from or for light pediatric recipients. Previous studies reported processing of low-volume bone marrow using other cell separator systems with thirdparty RBC-manipulation or ficoll density centrifugation.…”