Abnormalities of mitochondrial physiology and phenotype in sensory neurons in diabetes

Mechanisms of disease

“…DRG homogenates or purified mitochondrial preparations from DRG were isolated from STZ-diabetic rats and the rate of oxygen consumption (a measure of rate of respiratory chain activity -equivalent to rates of mitochondrial electron transfer that maintain oxidative phosphorylation) and activity of mitochondrial complexes were assessed. 3D in Chowdhury et al, 2010). At 12 and 16 weeks of diabetes there was no diminishment of mitochondrial respiratory chain activity; however, at 22 weeks a significant reduction was detected that was prevented by insulin therapy (Fig.…”

“…Figure 25.5B and C illustrates the effect of time and diabetes on the rates of oxygen consumption of DRG homogenates (coupled oxidative phosphorylation and activity of Complex IV). 6 in Chowdhury et al, 2010). 25.5) (Chowdhury et al, 2010).…”

“…The Fernyhough laboratory recently published the first full description of impaired mitochondrial bioenergetics in lumbar DRG of 12-22 week STZ-diabetic rats (Chowdhury et al, 2010). The Fernyhough laboratory recently published the first full description of impaired mitochondrial bioenergetics in lumbar DRG of 12-22 week STZ-diabetic rats (Chowdhury et al, 2010).…”

“…The Fernyhough laboratory recently published the first full description of impaired mitochondrial bioenergetics in lumbar DRG of 12-22 week STZ-diabetic rats (Chowdhury et al, 2010). 25.5) (Chowdhury et al, 2010). Figure 25.5B and C illustrates the effect of time and diabetes on the rates of oxygen consumption of DRG homogenates (coupled oxidative phosphorylation and activity of Complex IV).…”

“…(Reproduced fromChowdhury et al, 2010Chowdhury et al, , pp. Measurements of oxygen consumption were performed with energetic substrates, pyruvate, and malate as described inFig.…”