Abnormalities of erythrocyte membrane fluidity, lipid composition, and lipid peroxidation in systemic sclerosis: Evidence of free radical–mediated injury

Solans, Roser; Motta, Claude; Solà, Rosa; Ville, A.E. La; Lima, Joan; Simeón, Pilar; Montellà, Nuria; Armadans-Gil, Lluı́s; Fonollosa, Vicent; Vilardell, M

doi:10.1002/1529-0131(200004)43:4<894::aid-anr22>3.0.co;2-4

Cited by 73 publications

(34 citation statements)

References 36 publications

(49 reference statements)

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“…Considering that PUFAs are the fatty acids most susceptible to oxidative damage, and that their relative concentration could be considered as an indirect marker of membrane lipid peroxidation (Frei et al 1988;Grandjean et al 1998;Suarez et al 1999;Solans et al 2000;Griffiths et al 2001), an increase in PUFAs suggests that despite a presumable increase in ROS production, peroxidative erythrocyte damage was prevented, probably due to enhanced antioxidant capacity of RBCs. However, since in our study the climbers' nutritional regimen was not controlled, it cannot be established whether or not these results were also due to significant changes in dietary habits, favouring the increased consumption of lipid-rich macronutrients, which could compensate an eventual increase in lipid peroxidation.…”

Section: Discussionmentioning

confidence: 99%

Effect of a high-altitude expedition to a Himalayan peak (Pumori, 7,161�m) on plasma and erythrocyte antioxidant profile

Magalhães

Ascensão²,

Marques

et al. 2004

Eur J Appl Physiol

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The effects of a high-altitude exposure were studied in six mountaineers who spent 3 weeks at an altitude range between 5,250 and 7,161 m after 1 week in an acclimatization trek (2,800-5,250 m). Blood drawn from the antecubital vein was collected at sea level 1 day before and 1 day after the expedition to analyse some haematological variables [haemoglobin (Hb), haematocrit (Htc) and red blood cell (RBC) count], erythrocyte antioxidant enzyme activity [superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (Gr)] and membrane fatty acid profile [mono-unsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), saturated fatty acids (SFA), trans fatty acids (TRANS)]. Moreover, total antioxidant status (TAS), thiobarbituric acid reactive substances (TBARS), thiol protein groups (SH), SOD, GPx and Gr were measured in plasma. High-altitude exposure induced polycythaemia, with significant increases in RBC count (5.26%), Hb concentration (4.83%) and Htc (6.26%). Furthermore, a significant increase in plasma TBARS, SOD and Gr was observed after the expedition, whereas SH, TAS and GPx decreased. Erythrocyte glutathione-cycle-related antioxidant enzyme activity was upregulated, whereas SOD activity was maintained after the expedition. In addition, despite the unchanged (MUFA+PUFA)/SFA ratio, the membrane erythrocyte fatty acid content showed a significant increase in PUFAs and a decrease in TRANS, suggesting enhanced membrane fluidity. In conclusion, it seems that high-altitude exposure, besides quantitative variations in RBC expression, induced plasma oxidative stress and damage, and significant changes in erythrocyte components, namely in antioxidant enzyme activity and membrane fatty acid profile that might modify RBC functionality.

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Section: Discussionmentioning

confidence: 99%

Effect of a high-altitude expedition to a Himalayan peak (Pumori, 7,161�m) on plasma and erythrocyte antioxidant profile

Magalhães

Ascensão²,

Marques

et al. 2004

Eur J Appl Physiol

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“…Reactive oxygen species can also increase vascular smooth muscle differentiation, leading to a more contractile phenotype of these cells [49]. Several studies have demonstrated evidence of oxidative stress by various methodologies in SSc [47,[50][51][52][53]. Specifically, one recent study noted increased urinary F2-isoprostanes in the urine of SSc patients and this associated with nailfold capillary pattern and lung disease severity [54].…”

Section: Oxidative Stressmentioning

confidence: 99%

Microvascular damage in systemic sclerosis: Detection and monitoring with biomarkers

Hummers¹

2006

Curr Rheumatol Rep

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Vascular disease is universal in patients with systemic sclerosis (SSc), but there is a wide variability in its severity. It is clear that there is an early insult to the microvasculature, followed-up by on-going chronic process. This results in profound vascular damage in a subset of patients who develop severe events such as digital loss and pulmonary arterial hypertension. Although there is abundant evidence of vascular perturbation from studies of peripheral blood in SSc, there are few data about the ability to use these biomarkers to predict vascular outcomes. This paper examines the possibility of using circulating biomarkers to assess vascular disease activity and to predict severe vascular events among patients with SSc.

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“…Under oxidative stress PUFAs are highly susceptible to lipid peroxidation (Solans et al, 2000). Lipid peroxidation is a well-established mechanism of cellular injury and is used as an indicator of oxidative stress in cells and tissues (Magni et al, 1994).…”

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confidence: 99%

Malondialdehyde levels in serum of dogs infected with Babesia canis

Crnogaj¹,

Petlevski²,

Mrljak³

et al. 2010

Vet. Med.

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Babesiosis is a common tick born disease of dogs in tropical and subtropical regions of the world caused by different species of Babesia. The aim of the present study was to confirm the presence of oxidative stress by examining serum malondialdehyde (MDA), an end product of lipid peroxidation, in 35 dogs naturally infected with Babesia canis (B. canis). MDA was examined in 14 healthy dogs as well. Blood smears were prepared from peripheral blood and they showed the presence of B. canis in infected dogs. B. canis was confirmed using the PCR (Polymerase chain reaction) method. On the basis of clinical and laboratory data the 35 infected dogs were clinically classified into two groups, complicated (seven dogs) and uncomplicated (28 dogs). The noted complications were renal dysfunction (5/7), hepatic dysfunction (3/7), muscular involvement (2/7) and ARDS (1/7). Levels of blood urea nitrogen concentration (BUN), creatinin, total bilirubin, alanin aminotranspherase (ALT), alkaline phosphatase (AP), and gammaglutamil aminotranspherase (GGT) were significantly increased in dogs with complicated versus uncomplicated babesiosis. Furthermore the uncomplicated group of dogs was, depending on the severity of anaemia, classified as suffering from severe, moderate or mild disease. Levels of serum MDA were significantly higher in sick dogs (36.90 μmol/l ± 13.95) than healthy animals (8.13 μmol/l ± 1.78). There was no significant statistical difference in serum MDA levels between dogs with complicated (38.48 μmol/l ± 12.11) and uncomplicated babesiosis (36.50 μmol/l ± 14.55). Comparison of the groups based on the severity of anaemia showed that there was no significant statistical difference in serum MDA levels between them. The study demonstrated the involvement of oxidative damage in dogs naturally infected with B. canis.

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Abnormalities of erythrocyte membrane fluidity, lipid composition, and lipid peroxidation in systemic sclerosis: Evidence of free radical–mediated injury

Abstract: Our findings support the idea that oxidative injury occurs in SSc and that, through lipid peroxidation, it induces structural and functional changes of the EM that may contribute to the development of the microvascular abnormalities that are seen in the disease.

Cited by 73 publications

References 36 publications

Effect of a high-altitude expedition to a Himalayan peak (Pumori, 7,161�m) on plasma and erythrocyte antioxidant profile

Effect of a high-altitude expedition to a Himalayan peak (Pumori, 7,161�m) on plasma and erythrocyte antioxidant profile

Microvascular damage in systemic sclerosis: Detection and monitoring with biomarkers

Malondialdehyde levels in serum of dogs infected with Babesia canis

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