Abnormal β-catenin expression in oral cancer with no gene mutation: correlation with expression of cyclin D1 and epidermal growth factor receptor, Ki-67 labeling index, and clinicopathological features

Odajima, Tetsuyo; Sasaki, Yasushi; Tanaka, Nobuyuki; Kato‐Mori, Yuko; Asanuma, Hiroko; Ikeda, Tatsuru; Satoh, Masaaki; Hiratsuka, Hiroyoshi; Tokino, Takashi; Sawada, Norimasa

doi:10.1016/j.humpath.2004.12.009

Cited by 55 publications

(49 citation statements)

References 29 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, it was not detected a positive relationship of this protein with the tumor metastatic potential, which corroborates the results of previous studies 1,5,10,11 . However, some studies were able to establish a statistically significant relationship between the decreased ß-catenin expression with metastasis [12][13][14] .…”

Section: Resultsmentioning

confidence: 99%

Correlation of β-catenin expresssion and metastasis in tongue squamous cell carcinoma

Freitas

Silveira

et al. 2010

Acta Cir. Bras.

View full text Add to dashboard Cite

Purpose: It has been reported that the oral squamous cell carcinoma (OSCC) in tongue shows a more infiltrative profile, aggressive clinical course and poor prognosis, which may be related to a higher metastatic potencial. The aim of the present study was to assess the expression of ß-catenin in OSCC of the tongue and its correlation with tumor metastasis. Methods: Twenty four cases were selected and divided in two groups: metastatic group (n=12) and non-metastatic group (n=12). A semi-quantitative analysis of the ß-catenin expression was performed in the invasive tumor front and cases were graded as follows: negative (score 0), positive (score +), and strongly positive (score ++). Results: It was detected that 33%, 50% and 17% of the cases in metastatic group were scored 0, + and ++, respectively, and the non-metastatic group showed that 42% were scored "0", 33% scored + and 25% scored ++. Statistical analysis showed no diference between the studied groups. Conclusions: Based on these results, it can be concluded that the immunoexpression of ß-catenin does not represent a valuable tool to predict metastatic potencial of OSCC in tongue. Key words: Carcinoma, Squamous Cell. Tongue. Neoplasm Metastasis. beta Catenin. Cell Adhesion. RESUMOObjetivo: O carcinoma de células escamosas (CCE) quando presente em língua exibe aspecto mais infiltrativo, curso clínico agressivo e prognóstico desfavorável. Tais características podem associar-se com maior potencial metastático. O presente trabalho teve como meta investigar a expressão de ß-catenina em CCE de língua e sua relação com o desenvolvimento de metástases. Métodos: Foram selecionados 24 casos de CCE em língua e divididos em dois grupos: grupo metastático (n=12) e grupo sem metástase (n=12). Realizou-se uma análise semi-quantitativa da imunoexpressão da ß-catenina no fronte invasivo tumoral e sendo considerada como negativa, positiva e fortemente positiva, atribuindo-se os escores, "0", "+" e "++", respectivamente. Resultados: A expressão de ß-catenina apresentou, no grupo metastático, escore "0" em 33% dos casos, escore "+" em 50% dos casos e escore "++" em 17% dos casos; e, no grupo sem metástase, escore "0" em 42% dos casos, escore "+" em 33% dos casos e escore "++" em 25% dos casos. A análise estatística não demonstrou nenhuma diferença signficativa entre a expressão da proteína nos dois grupos. Conclusão: Diante destes resultados, concluiu-se que a expressão da ß-catenina não constitui um método eficaz, isoladamente, para predizer o potencial metastático do CCE em língua. Descritores: Carcinoma de Células Escamosas. Língua. Metástase Neoplásica. beta Catenina. Adesão Celular.

show abstract

Section: Resultsmentioning

confidence: 99%

Correlation of β-catenin expresssion and metastasis in tongue squamous cell carcinoma

Freitas

Silveira

et al. 2010

Acta Cir. Bras.

View full text Add to dashboard Cite

show abstract

“…Overexpression of cyclin D1 has been found in both human and rat oral tumors (27,28). Overexpression of COX2 has been found in human oral cancer and DMBA induced hamster cheek pouch tumor (29,30).…”

Section: Discussionmentioning

confidence: 99%

Enhancement of tongue carcinogenesis in Hras128 transgenic rats treated with 4-nitroquinoline 1-oxide

Naoi

Sunagawa²,

Yoshida³

et al. 2009

Oncol Rep

View full text Add to dashboard Cite

Abstract. Transgenic rats carrying human c-Ha-ras protooncogene (Hras128 rats) have been shown to be highly susceptible to induction of tumors. We have found an early induction of tongue tumors in Hras128 rats treated with 4-nitroquinoline 1-oxide (4NQO). 4NQO was administered to the Hras128 and wild-type Sprague-Dawley (SD) rats for 4 and 8 weeks, respectively. The experiment was terminated at 14 (Hras128 rats) and 28 (SD rats) weeks. Either during or after treatment with 4NQO, dysplastic hyperplasia, papilloma and squamous cell carcinoma were found on the tongue of both Hras128 and wild-type rats, with a higher incidence and multiplicity in Hras128 rats. Treatment of the Hras128 rats with 4NQO significantly increased cell proliferation in the tumor compared to the control rats. In the tongue tumors of the Hras128 rats, there was a significant increase in the mRNA expression levels of cyclin D1 and COX2. To examine whether this experimental system is useful for screening of the candidate agents for cancer preventive effect, nimesulide, a selective COX2 inhibitor, was tested in the present model. Nimesulide significantly decreased total multiplicity of tongue lesions compared to the control rats. Treatment of Hras128 rats with nimesulide caused a significant decrease in the levels of mRNA expression of cyclin D1 and COX2 in the tumor. Therefore, the current 4NQO-induced Hras128 rat tongue carcinogenesis model provides a simple and rapid system for investigating carcinogenesis process and evaluating the effect of possible cancer preventive agents for human tongue cancer.

show abstract

“…The key step in this alteration is the detachment of β-catenin from its membrane-associated partner, cadherin, which results in an elevation of the β-catenin cytoplasmic level (26). This may promote free β-catenin signaling along with the subsequent cell growth and differentiation (8,27).…”

Section: M475271 Protects Ve-cadherin/ β-Catenin Association From Vegmentioning

confidence: 99%

The Novel Src Kinase Inhibitor M475271 Inhibits VEGF-Induced Vascular Endothelial-Cadherin and β-Catenin Phosphorylation but Increases Their Association

et al. 2006

View full text Add to dashboard Cite

Abstract. M475271, 4-quinazolinamine, N-(2-chloro-5-methoxyphenyl)-6-methoxy-7-[(1-methyl-4-piperidinyl) methoxy]-(9Cl), is a new anilinoquinazoline derivative that displays selective inhibition of Src kinase activity and tumor growth in vivo. Vascular endothelial growth factor (VEGF)-induced angiogenesis plays a pivotal role in tumor growth and metastasis. Vascular endothelial (VE)-cadherin is an endothelial cell-specific adhesion molecule that can interact with the cytoskeleton via several anchoring molecules such as β-catenin. Here, we examined the effect of M475271 on VE-cadherin and β-catenin phosphorylation and association. We also examined its effect on VEGF-induced human umbilical vein endothelial cell (HUVEC) proliferation, migration, and tube formation. The findings reveal pretreatment with M475271 significantly inhibits VEGF-induced VE-cadherin and β-catenin phosphorylation. However, M475271 significantly increases VE-cadherin and β-catenin association compared to the VEGFtreated group. Confocal laser microscopic examination confirmed the augmentation effect of M475271 on VE-cadherin and β-catenin association. Finally, M475271 was shown to have inhibitory effects comparable to those of PP2 and Herbimycin A on VEGF-induced HUVEC proliferation, migration, and tube formation. These findings suggest that M475271 attenuates VEGF-induced angiogenesis by maintaining cell-cell junction stability. Although the involvement of other signaling molecules cannot be ruled out, M475271 has potential as a drug for the inhibition of the angiogenesis needed for tumor growth and metastasis.

show abstract

Abnormal β-catenin expression in oral cancer with no gene mutation: correlation with expression of cyclin D1 and epidermal growth factor receptor, Ki-67 labeling index, and clinicopathological features

Cited by 55 publications

References 29 publications

Correlation of β-catenin expresssion and metastasis in tongue squamous cell carcinoma

Correlation of β-catenin expresssion and metastasis in tongue squamous cell carcinoma

Enhancement of tongue carcinogenesis in Hras128 transgenic rats treated with 4-nitroquinoline 1-oxide

The Novel Src Kinase Inhibitor M475271 Inhibits VEGF-Induced Vascular Endothelial-Cadherin and β-Catenin Phosphorylation but Increases Their Association

Contact Info

Product

Resources

About