Abnormal octadeca-carbon fatty acids distribution in erythrocyte membrane phospholipids of patients with gastrointestinal tumor

Background Platelet membranes are extremely susceptible to peroxidation, forming a variety of lipid peroxides, including malondialdehyde (MDA), which has been implicated in the etiology of cardiovascular diseases. Moreover, platelet-leukocyte aggregates (PLAs) are known to contribute to advanced endothelial injury and atherogenesis. Material/Methods Fatty acid (FA) methyl esters of the platelet membranes of 79 apparently healthy men without any acute clinical condition at the time of the study were identified by GC/MS. MDA was measured by HPLC in blood serum, and PLAs were analyzed by whole-blood flow cytometry. Individuals were divided into quartiles according to MDA concentration and percentage of PLAs formation. The composition of platelet membrane FAs was compared to MDA concentration and the percentage of PLAs formation in apparently healthy individuals. Results In quartiles (Q) with higher MDA concentration, percentage of C 16: 1ω7 (Q 1 vs. Q 3 , p=0.021), C 20: 1ω9 (Q 2 vs. Q 4 , p=0.028) and C 20: 5ω3 (Q 2 vs. Q 4 , p=0.046) was lower. However, C 22: 5ω3 (Q 1 vs. Q 4 , p=0.038) and total ω3 (Q 1 vs. Q 2 , p=0.024) were higher. Conclusions MDA and the formation of platelet-monocyte aggregates stimulate the incorporation of monounsaturated fatty acids and polyunsaturated fatty acids in platelet phospholipid membranes, which may be a hallmark for a changed level of biologically active compounds required for the activation of future platelets.

show abstract

“…C 18: 1ω9 accounts for the highest percentage of FA compared to other MUFAs [25,26,28]. The same tendency was observed in our data.…”

Section: Discussionsupporting

confidence: 91%

“…According to our data, the highest percentage of FAs in the platelet phospholipid membrane consisted of SFA. Other authors have obtained very similar results [25][26][27][28]. Moreover, C16: 0 is reported to be the main FA of the platelet membrane [29], as was observed in our study.…”

Section: Discussionsupporting

confidence: 91%

Association of Platelet Membrane Fatty Acid Composition with Markers of Oxidative Stress in Healthy Men

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Alterations in the FA composition of erythrocyte membranes were linked to an increased risk of cancer development [ 36 , 37 ]. In a prospective study conducted in China, the FA profile of erythrocyte membranes in gastrointestinal cancer patients was found to be significantly different from that in healthy controls [ 38 ]. A case–control study within the EPIC Cohort Study discovered that a specific erythrocyte membrane phospholipid FA profile, possibly reflecting both a complicated dietary pattern and altered FA metabolism, was related to progressed colorectal adenoma risk [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Mediation analysis of erythrocyte lipophilic index on the association between BMI and risk of oral cancer

et al. 2022

View full text Add to dashboard Cite

Aims To explore the relationship between the fatty acid lipophilic index (LI) of the erythrocyte membrane and oral cancer risk, as well as to evaluate the possibility of LI acting as a mediator of the association between body mass index (BMI) and oral cancer. Method Twenty-three fatty acids (FAs) of the erythrocyte membrane were measured using gas chromatography in 380 patients with oral cancer and 387 control subjects. The LI was calculated based on the FA proportion and FA melting points. The association of BMI and erythrocyte LI with oral cancer risk was analysed using logistic regression. The mediation effect of LI on the association between BMI and oral cancer risk was evaluated using mediation analysis. Results Among the control group, 46.0% were overweight or obese, which was significantly higher than that of oral cancer patients (29.5%). Significant differences in erythrocyte membrane saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs) were observed between the patient and control groups. The proportion of C18:1 n-9 from the MUFA family increased in oral cancer patients (12.67%) compared with controls (12.21%). While the total proportion of n-3 PUFAs decreased in oral cancer patients compared with controls, with C20:5 n-3 decreasing from 0.66 to 0.47%, and C22:6 n-3 decreasing from 5.82 to 4.86%. The LI was lower in the control participants (M = 27.6, IQR: 27.3–27.9) than in the oral cancer patients (M = 28.2, IQR: 27.9–28.5). BMI was inversely associated with oral cancer risk with a fully adjusted OR of 0.59 (95% CI: 0.43–0.83), while LI was positively associated with oral cancer risk with a fully adjusted OR of 1.99 (95% CI:1.36–2.94). LI explained 7% of the variance in the relationship between BMI and oral cancer risk. Conclusions The distribution of the FA profile in erythrocyte membranes differed between the oral cancer patients and the control group. The LI derived from the profile of FAs was positively associated with the risk of oral cancer, and the associations between BMI and oral cancer risk can be explained, at least in part, by LI.

show abstract

Abnormal octadeca-carbon fatty acids distribution in erythrocyte membrane phospholipids of patients with gastrointestinal tumor

Abstract: Supplemental Digital Content is available in the text

Cited by 2 publications

References 30 publications

Association of Platelet Membrane Fatty Acid Composition with Markers of Oxidative Stress in Healthy Men

Association of Platelet Membrane Fatty Acid Composition with Markers of Oxidative Stress in Healthy Men

Mediation analysis of erythrocyte lipophilic index on the association between BMI and risk of oral cancer

Contact Info

Product

Resources

About