Abnormal gonad development in Kit W-2Bao mice caused by a Kit gene missense mutation

Abstract

Kit W-2Bao mice are single-gene autosomal dominant mutation mice with a B6 background that were bred in our laboratory. Heterozygotes had morphological characteristics including albinism of the abdomen, extremities, and tail, whereas the homozygotes had albinism of the body, black eyes, and infertility. The homozygous mutants showed small, structurally abnormal gonads, and lacked germ cells. Heterozygous male mice lacked germ cells in some contorted seminiferous tubules. This mutation has been mapped at 43.8 cM from the centromere in chromosome 5 by linkage analysis and Kit has been identified as the candidate gene. After Kit full-length mRNA amplification, it was found that a G to T conversion at position 1228 in the ORF changed the 410th amino acid from V to F. This amino acid change could affect the protein's secondary structure. Heterozygous mutant mice were intercrossed and homozygous mutant mice were bred and genotyped. We found that no primordial germ cells (PGCs) appeared in the urogenital ridge area at fetus day 11.5 in the homozygotes. The number of PGCs also significantly decreased in heterozygotes. At fetus day 15.5, the differentiation of the testis tubule structure was unclear; as well, they contained no spermatogonia. Female homozygotes contained no primordial follicles in the ovary. The numbers of PGCs and primordial follicles were significantly decreased in heterozygous mice. W -2Bao is the only mutated site in the extracellular 4th Ig-like domain and this mutant mouse model provides new material for the study of the mechanism of reproductive system development. mouse, Kit, missense mutation, gonad Citation: Wu B J, Yin L J, Lu Z L, et al. Abnormal gonad development in Kit W-2Bao mice caused by a Kit gene missense mutation.

Research on Edible Fungi and Algae as Feed Supplement

“…Polysaccharides are helpful to enhance cell proliferation ability of bone, accelerate thymus and spleen cell growth by thickening cortical thickness [43]. Even if the organism is in the condition of lipid peroxidation, microalgae could decrease blood lipid and increasing antioxidase activities by reducing the serum triglyceride (TG), total cholesterol (TC) and enhancing the activities of catalase (CAT), superoxide dismutase (SOD) [44]. Hydrogen peroxide, a kind of free radical, can remarkably induce cell death, while protein waste from algae has protection effects on oxidation-induced DNA damage [45].…”

Phenotype analysis and mutant gene location of ventral yellow mouse (<I>VY</I><SUP><I>Slac</I></SUP>)

“…的突变系小鼠通常是在已经建立的近交系中, 由一 次突变建立起来的。突变系小鼠与背景品系之间表 型上的差异正是由一个或少数几个突变基因的差 别导致的, 代表了相关突变基因的功能 (Wu et al, 2009(Wu et al, , 2010)。众多的突变系小鼠不仅仅是功能基因 研究的好材料, 也是人类疾病模型开发的资源库, 而发现、培育具有特定表型的突变系小鼠是相关工 作的基础 (Morgan et al, 2010;Wilkinson et al, 2010 12/12 h 明暗交替。 1.2 皮肤切片的多巴染色、 毛发镜检及主要脏器的 组织病理学研究 取 7 日龄腹侧黄斑小鼠、B 6 小鼠和 BALB/c 小 鼠的背部及腹部约 1.5 cm×0.5 cm 大小皮片, 按照 文献所介绍多巴染色步骤进行切片、染色、观察 (Gong & Zhan, 1994)。取 2 月龄腹侧黄斑小鼠和 B 6 两种小鼠背部及腹部的被毛, 经二甲苯湿润后摊 片、中性树胶封固, 光镜下观察。取 2 月龄腹侧 黄斑小鼠和 B 6 小鼠, 解剖观察大体结构, 并取脑、 心、肝、脾、肺、肾、胸腺、肾上腺、睾丸附睾、 子宫卵巢等脏器组织, 常规制作石蜡切片、染色 镜检。…”