Abnormal Forms of the Herpes Simplex Virus Immediate Early Polypeptide Vmw175 Induce the Cellular Stress Response

Russell, Jackie; Stow, Elizabeth C.; Stow, Nigel D.; Preston, Chris M.

doi:10.1099/0022-1317-68-9-2397

Cited by 29 publications

(20 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6a, lane 4). Complexes a and a ÷ were not formed with extracts from cells infected with virus in1411, which does not express Vmw175 (Russell et al, 1987). The results using extracts from cells infected with d11403 (which does not express Vmw110; Stow & Stow, 1986) were the same as those with wild-type virus.…”

Section: The Mutations In Ts1219 Ts1221 and Ts1225 Affect Conserved supporting

confidence: 59%

“…Mutant tsK, with a single base substitution in IE gene 3, has previously been characterized (Marsden et al, 1976;Preston, 1981). Virus inl411, with a stop codon inserted near the amino terminal end of the Vmw 175 coding sequence, has been described by Russell et al (1987). Virus d11403, with a large deletion in the coding region of both copies of IE gene 1, has been described by Stow & Stow (1986 (Sanger et al, 1977(Sanger et al, , 1980 Marsden et al (1978).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A mutant of herpes simplex virus type 1 immediate early polypeptide Vmw175 binds to the cap site of its own promoter in vitro but fails to autoregulate in vivo

Paterson¹,

Preston²,

Everett³

1990

Journal of General Virology

View full text Add to dashboard Cite

Section: The Mutations In Ts1219 Ts1221 and Ts1225 Affect Conserved supporting

confidence: 59%

Section: Methodsmentioning

confidence: 99%

A mutant of herpes simplex virus type 1 immediate early polypeptide Vmw175 binds to the cap site of its own promoter in vitro but fails to autoregulate in vivo

Paterson¹,

Preston²,

Everett³

1990

Journal of General Virology

View full text Add to dashboard Cite

“…Four isolates with restriction endonuclease cleavage profiles indistinguishable from wild-type (wt) HSV-1 were selected. The HSV-1 insertion mutant inl411, which has an 8 bp oligonucleotide linker insertion which also creates an in-frame amber termination codon in the Vmwl75 coding sequences, was constructed and propagated as described elsewhere (Russell et al, 1987).…”

Section: Methodsmentioning

confidence: 99%

Herpes Simplex Virus Genes Involved in Latency in vitro

Russell

Stow²,

Stow³

et al. 1987

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

SUMMARYThe properties of temperature-sensitive (ts), insertion or deletion mutants of herpes simplex virus (HSV) were investigated in an in vitro model system for latency. The studies defined virus gene products required for establishment of latency and for reactivation of latent virus. All mutants tested established latency in human foetal lung fibroblasts and could be reactivated by intertypic superinfection with HSV or with human cytomegalovirus. Two mutants of HSV type 1 used in these studies, tsK and in 1411, failed to synthesize active immediate early (IE) polypeptide Vmw175 and were blocked at a very early stage of the virus replication cycle, showing that, at most, only limited gene expression is necessary for the establishment of latency. Mutant dl1403, which lacks the gene encoding IE polypeptide Vmwll0, established latency as efficiently as wild-type HSV. Latent HSV type 2 was reactivated by superinfection with tsK or in1411 but not with dl1403, suggesting that polypeptide Vmw110, which is known to regulate gene expression by trans-activation, is required for reactivation in the in vitro system.

show abstract

“…These hypotheses are also compatible with the fact that abnormal proteins [50] and many viral infections (reviewed in [51] ) can act as stress signals in eukaryotic cells. In particular, this has been well documented in various HSV systems [52][53][54][55][56][57][58][59]. In addition, in some experiments, we used SIV as a control virus.…”

Section: /V2mentioning

confidence: 99%