Abnormal expressions of ADAMTS-1, ADAMTS-9 and progesterone receptors are associated with lower oocyte maturation in women with polycystic ovary syndrome

GohariTaban, Sepide; Amiri, Iraj; Asl, Sara Soleimani; Saidijam, Massoud; Yavangi, Mahnaz; Khanlarzadeh, Elham; Mohammadpour, Nooshin; Shabab, Nooshin; Artimani, Tayebe

doi:10.1007/s00404-018-4967-2

Cited by 17 publications

(16 citation statements)

References 30 publications

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“…In clinical situations, evaluating oocyte quality for IVF/ICSI and choosing which embryo to transplant are vital considerations for COH women. Previous studies have shown that ADAMTS1, whose expression is LH/hCG and follicle-size dependent, and which shows rapidly induced expression (>20fold increase in CCs after hCG treatment in humans) [42,43], was related to follicular development and ovulation and may be involved in female infertility such as PCOS [3,7,8,44,45]. PCOS patients have lower ADAMTS1 levels in CCs and higher ADAMTS1 levels in follicular fluids compared with normal ovulatory women [4,25,26].…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, several investigators have demonstrated a close correlation between ADAMTS1 levels in human CCs or follicle fluids and impaired oocyte quality in polycystic ovary syndrome (PCOS) patients [23][24][25][26]. Consistent with ADAMTS1 −/− mice, ADAMTS1 levels in CCs were decreased in PCOS patients, which might contribute to abnormalities, such as low fertilization rate and cleavage rate [4,7,26]. To date, no study has evaluated the relationship between ADAMTS1 level and controlled ovarian hyperstimulation (COH) outcomes in normal ovulatory patients.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 90%

“…LH mainly triggers the ERK1/ ERK2 signaling cascade in granulosa cells (GCs) to induce the expression of many genes crucial for ovulation, oocyte meiosis, and extracellular matrix (ECM) remodeling [1]. Many of these ERK1/ERK2-dependent genes, including AREG, ADAMTS1, and CITED2, in GCs were associated with oocyte quality and IVF outcomes [2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

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ADAMTS1 and HSPG2 mRNA levels in cumulus cells are related to human oocyte quality and controlled ovarian hyperstimulation outcomes

Jin

Yang

et al. 2020

J Assist Reprod Genet

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Purpose The study investigated potential correlations between the expression levels of ADAMTS1 and HSPG2 in cumulus cells (CCs) and controlled ovarian hyperstimulation (COH) outcomes. Methods RT-PCR was used to determine ADAMTS1 and HSPG2 mRNA levels in mice CCs at different timepoints (0, 4, 8, 12, and 16 h) after human chorionic gonadotropin (hCG) injection, and in CCs after RNAi treatment. Women with polycystic ovary syndrome (PCOS) (n = 45) and normal ovulatory controls (n = 103) undergoing IVF/ICSI were recruited. Relative ADAMTS1 and HSPG2 mRNA levels were measured by RT-PCR. Moreover, correlations of ADAMTS1 and HSPG2 levels with COH outcomes were analyzed. Results At different timepoints after hCG treatment, ADAMTS1 mRNA had the highest level at 12 h, whereas HSPG2 showed opposite profiles to ADAMTS1 with the lowest level at 12 h. HSPG2 expression was upregulated after ADAMTS1 RNAi treatment The PCOS group had higher HSPG2 and lower ADAMTS1 expression levels than controls. In normal ovulatory women (control group), a higher expression of ADAMTS1 and lower expression of HSPG2 were associated with more mature oocytes, transplantable embryos, and good quality embryos, whereas higher transplantable embryo rates and good quality embryo rates were obtained only with lower HSPG2 expression. ROC curves showed the co-measurement of ADAMTS1 and HSPG2 had a better predictive power than separate analyses. Conclusion The dynamic profiles of ADAMTS1 and HSPG2 were inversely correlated in CCs. In PCOS and normal ovulatory patients, higher ADAMTS1 and lower HSPG2 expression levels in CCs were related to better COH outcomes.

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Section: Discussionmentioning

confidence: 99%

Section: Electronic Supplementary Materialsmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

ADAMTS1 and HSPG2 mRNA levels in cumulus cells are related to human oocyte quality and controlled ovarian hyperstimulation outcomes

Jin

Yang

et al. 2020

J Assist Reprod Genet

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“…Importantly, the expression level of ADAMTS1 is shown to be associated with the oocyte quality in PCOS patients (Huang et al, 2013;GohariTaban et al, 2019). It has also been confirmed that ADAMTS1 and its substrate, cleaved VCAN1, dramatically increase during COC maturation from the germinal vesicle (GV) to MII stage (Bo and Julang, 2018), which suggests a key role of ADAMTS1 in the maturation of oocytes.…”

Section: Discussionmentioning

confidence: 88%

Expression Level of ADAMTS1 in Granulosa Cells of PCOS Patients Is Related to Granulosa Cell Function, Oocyte Quality, and Embryo Development

Guang

Yao

et al. 2021

Front. Cell Dev. Biol.

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A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1) is an extracellular matrix metalloproteinase that plays an important role in the process of ovulation. According to previous studies, the expression level of ADAMTS1 in the granulosa cells of polycystic ovarian syndrome (PCOS) patients and the mechanism for regulating oocyte quality and embryonic development potential are still unclear. Our research clarified that ADAMTS1 was significantly increased in granulosa cells of PCOS patients as compared to ovulatory controls. After silencing ADAMTS1 in granulosa cells, cell proliferation and E2 secretion were significantly inhibited, which may be related to the down-regulation of B-cell lymphoma 2 (Bcl2) family genes and key genes involved in E2 synthesis. Through retrospective analysis of the clinical data, it was found that the expression level of ADAMTS1 was significantly positively correlated to the oocyte maturation rate and good-quality embryo rate in PCOS patients. The downregulation of ADAMTS1 in primary granulosa cells lead to the changes in the expression of marker genes for oocyte and embryonic quality. By using immunofluorescence staining, it was found ADAMTS1 was expressed in various stages of pre-implantation embryo but its expression level gradually decreases with the development of the embryo. In addition, the silence of ADAMTS1 in 3PN zygotes significantly prolonged the development time of the zygote to the morula stage. This is, to our knowledge, the first time to explored the mechanism by which ADAMST1 is involved in affecting the quality of oocytes and embryonic development potential, which will provide new evidence for further understanding of the follicular microenvironment and embryo development.

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“…This study was a part of a project approved by the Ethics Committee of the Hamadan University of Medical Sciences (IR.UMSHA. (11). The data were analyzed using SPSS software version 16.…”

Section: Methodsmentioning

confidence: 99%

ADAMTS proteoglycanases downregulation with impaired oocyte quality in PCOS

Taban

Amiri

Saidijam

et al. 2021

Archives of Endocrinology and Metabolism

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Objective: A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4) and ADAMTS-5 normal expression levels are essential for ovulation and subsequent fertilization. The objective of the present study was to assess expression pattern of these genes in cumulus cells (CCs) taken from patients with polycystic ovary syndrome (PCOS) and to investigate any possible relationship with the oocyte quality. Subjects and methods: ADAMTS-4 and -5 expression levels within CCs containing oocytes at the metaphase II (MII) and germinal vesicle (GV) stages, taken from 35 patients with PCOS and 35 women with normal ovarian function, were investigated using RT-qPCR. Moreover, possible correlations between ADAMTS-4, ADAMTS-5, and progesterone receptors (PRs) expression as well as oocyte quality were evaluated. Results: ADAMTS-4 and -5 expression levels were dramatically diminished in the CCs of the PCOS patients when compared to the controls. ADAMTS-4 and -5 expression levels were correlated with each other and with the oocyte quality. Furthermore, lower expression levels of ADAMTS-4 and -5 in the PCOS patients were strongly correlated with the diminished PRs expression levels. Conclusions: Downregulation of ADAMTS-4 and -5 in the human CCs of the PCOS patients correlated with the decline in the PRs expression, and impaired oocyte quality may cause lower oocyte recovery, maturation, and fertilization rate.

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Abnormal expressions of ADAMTS-1, ADAMTS-9 and progesterone receptors are associated with lower oocyte maturation in women with polycystic ovary syndrome

Cited by 17 publications

References 30 publications

ADAMTS1 and HSPG2 mRNA levels in cumulus cells are related to human oocyte quality and controlled ovarian hyperstimulation outcomes

ADAMTS1 and HSPG2 mRNA levels in cumulus cells are related to human oocyte quality and controlled ovarian hyperstimulation outcomes

Expression Level of ADAMTS1 in Granulosa Cells of PCOS Patients Is Related to Granulosa Cell Function, Oocyte Quality, and Embryo Development

ADAMTS proteoglycanases downregulation with impaired oocyte quality in PCOS

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