Abnormal Expression of Differentiation Related Proteins and Proteoglycan Core Proteins in the Urothelium of Patients With Interstitial Cystitis

Hauser, Paul; Dozmorov, Mikhail G.; Bane, Barbara L.; Slobodov, Gennady; Culkin, Daniel J.; Hurst, Robert E.

doi:10.1016/j.juro.2007.09.022

Cited by 112 publications

(102 citation statements)

References 20 publications

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“…Although we found a heterogeneous pattern of immunofluorescence for all patients with PBS/IC, our findings are in agreement with other researchers, that analyzed biomarkers by immunohistochemistry in the urothelium of patients with cystitis and found distinct patterns of marking, sometimes with predominance of either one of a set of matrix components, but all the profiles different from those found in normal urothelium (15,16). Whereas the inflammatory involvement of the urothelium does not occur uniformly, our current results and also those of Hauser et al (16) suggest different profiles of involvement and expression of various inflammatory components of the extracellular matrix and depending on the different stages of inflammation and/or remodeling that each segment of the bladder is subject.…”

Section: Discussionsupporting

confidence: 92%

“…Whereas the inflammatory involvement of the urothelium does not occur uniformly, our current results and also those of Hauser et al (16) suggest different profiles of involvement and expression of various inflammatory components of the extracellular matrix and depending on the different stages of inflammation and/or remodeling that each segment of the bladder is subject. That is why a pattern of GAG expression has not been achieved.…”

Section: Discussionsupporting

confidence: 68%

“…But one can not assure they are specific to PBS/IC. Variations are described in literature and this topic also remains controversial (9,15,16).…”

Section: Discussionmentioning

confidence: 99%

“…Urine and tissue levels of GAG have been studied in PBS/IC and in animal models but their results are not uniform (2,(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Such variation suggests that there is no complete understanding of how and why GAG is produced and degraded in PBS/IC, if GAG expression is the cause or the reaction for PBS/IC.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis

et al. 2014

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ARTICLE INFO ______________________________________________________________ ______________________Introduction: Painful bladder syndrome/interstitial cystitis (PBS/IC) pathogenesis is not fully known, but evidence shows that glycosaminoglycans (GAG) of bladder urothelium can participate in its genesis. The loss of these compounds facilitates the contact of urine compounds with deeper portions of bladder wall triggering an inflammatory process. We investigated GAG in urine and tissue of PBS/IC and pure stress urinary incontinence (SUI) patients to better understand its metabolism. Materials and Methods: Tissue and urine of 11 patients with PBS/IC according to NIDDK criteria were compared to 11 SUI patients. Tissue samples were analyzed by histological, immunohistochemistry and immunofluorescence methods. Statistical analysis were performed using t Student test and Anova, considering significant when p < 0.05. Results: PBS/IC patients had lower concentration of GAG in urine when compared to SUI (respectively 0.45 ± 0.11 x 0.62 ± 0.13 mg/mg creatinine, p < 0.05). However, there was no reduction of the content of GAG in the urothelium of both groups. Immunofluorescence showed that PBS/IC patients had a stronger staining of TGF-beta, decorin (a proteoglycan of chondroitin/dermatan sulfate), fibronectin and hyaluronic acid. Conclusion: the results suggest that GAG may be related to the ongoing process of inflammation and remodeling of the dysfunctional urothelium that is present in the PBS/IC.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 68%

“…But one can not assure they are specific to PBS/IC. Variations are described in literature and this topic also remains controversial (9,15,16).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis

et al. 2014

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“…38 Abnormal expression of differentiation related proteins (keratin 18 and 20) and proteoglycan core proteins (biglycan, decorin, perlecan and syndecan-1), which are found in BPS/IC bladders, also suggest a loss of barrier function proteins, and altered differentiation proteins are independent and occur independently of inflammation. 39 BUC in BPS/IC patients produce a unique APF, which is secreted into the urine of BPS/IC patients, whereas normal BUC do not; this APF inhibited proliferation of cultured BUC. 40 APF is a frizzled-related peptide growth inhibitor exclusively containing a transmembrane segment of a frizzled protein.…”

Section: Urothelial Differentiation Proteins and Proteoglycan Proteinmentioning

confidence: 94%

Potential urine and serum biomarkers for patients with bladder pain syndrome/interstitial cystitis

Kuo

2014

Int J of Urology

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Abbreviations & Acronyms APF = antiproliferative factor BPS/IC = bladder pain syndrome/interstitial cystitis BUC = bladder urothelial cells CRP = C-reactive protein EGF = epidermal growth factor GP51 = glycoprotein 51 HB-EGF = heparin binding growth factor-like growth factor IC = interstitial cystitis IL = interleukin MCP-1 = monocyte chemotactic protein-1 MIP-1β = macrophage inflammatory protein OAB = overactive bladder NGF = nerve growth factor NIDDK = National Institute of Diabetes, Digestive, and Kidney Diseases TNF-α = tumor necrosis factor-α ZO-1 = zonula occludens-1 Abstract: There is a lack of consensus on the pathophysiology of bladder pain syndrome/ interstitial cystitis. The chronic pain symptoms of bladder pain syndrome/interstitial cystitis refractory to local treatment could be a result of central nervous system sensitization and persisting abnormalities in the bladder wall, which activate the afferent sensory system. Evidence also shows that bladder pain syndrome/interstitial cystitis is a heterogeneous syndrome and that the two subtypes, the ulcerative (classic) and non-ulcerative types, represent different disease entities. There is a need for non-invasive markers for the differential diagnoses of the subtypes of bladder pain syndrome/interstitial cystitis, and between bladder pain syndrome/interstitial cystitis and bladder sensory disorders, such as hypersensitive bladder syndrome or overactive bladder. Bladder pain syndrome/interstitial cystitis, but not overactive bladder, involves an aberrant differentiation program in the bladder urothelium that leads to altered synthesis of several proteoglycans, cell adhesion and tight junction proteins, and bacterial defense molecules. These findings have led to the rationale for identifying urinary biomarkers to detect bladder pain syndrome/interstitial cystitis in patients with frequency urgency syndrome. Recently, the markers that have been the focus of the most research are antiproliferative factor, epidermal growth factor, heparin-binding epidermal growth factor, glycosaminoglycans and bladder nitric oxide. In addition, inflammatory proteins in the urine and serum play important roles in the pathogenesis of bladder pain syndrome/interstitial cystitis. The urinary proteome is an easily accessible source of biomarkers for differentiation between inflammatory bladder disorders. Analysis of multiple urinary proteins and serum cytokines could provide a diagnostic basis for bladder pain syndrome/interstitial cystitis, and could be a tool for the differential diagnosis of bladder pain syndrome/interstitial cystitis and other sensory bladder disorders.

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Interstitial Cystitis/Bladder Pain Syndrome

Han

Nguyen

Tennyson

et al. 2020

Female Sexual Pain Disorders

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Abnormal Expression of Differentiation Related Proteins and Proteoglycan Core Proteins in the Urothelium of Patients With Interstitial Cystitis

Cited by 112 publications

References 20 publications

Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis

Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis

Potential urine and serum biomarkers for patients with bladder pain syndrome/interstitial cystitis

Interstitial Cystitis/Bladder Pain Syndrome

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