Abnormal development of <i>Hymenolepis nana</i> larvae in immunosuppressed mice

Lucas, Sebastian; Hassounah, O.; Müller, R.; Doenhoff, Michael J.

doi:10.1017/s0022149x00006386

Cited by 28 publications

(18 citation statements)

References 10 publications

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“…The numbers of cysticercoids inoculated into mice in our study were consistent with other researchers who have reported successful infection to adult worm stages with 1±20 cysticercoids (Lucas et al, 1980;Ito et al, 1988;Kumazawa, 1992). Furthermore, in the present study, the consistent development of cysticercoids, obtained from the Japanese control isolate, into adult worms when inoculated into laboratory rodents indicated that the storage of cysticercoids in PBS prior to inoculation did not adversely affect the viability of the cysticercoids.…”

Section: Discussionsupporting

confidence: 91%

“…Based on previous research (Lucas et al, 1980;Ito et al, 1988;Asano et al, 1992;Maki & Ito, 1998) several different susceptible rodent hosts were used in the inoculation trials in this study. However, the use of these highly susceptible mice, even when further immunosuppressed with cortisone, and the use of hypothymic rodent hosts did not result in the development of eggs to either cysticercoid or adult worm stages in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…5. The pharmacological immunosuppression (Lucas et al, 1980) of mice, rats and hamsters using subcutaneous injections of cortisone acetate (Sigma, USA) as well as the use of a congenitally hypothymic mouse model (Ito, 1985). 6.…”

Section: Infection Methods Tested Using Human Isolatesmentioning

confidence: 99%

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Failure to infect laboratory rodent hosts with human isolates of Rodentolepis (= Hymenolepis) nana

et al. 2002

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Confusion exists over the species status and host-specificity of the tapeworm Rodentolepis (= Hymenolepis) nana. It has been described as one species, R. nana, found in both humans and rodents. Others have identified a subspecies; R. nana var. fraterna, describing it as morphologically identical to the human form but only found in rodents. The species present in Australian communities has never been identified with certainty. Fifty one human isolates of Rodentolepis (= Hymenolepis) nana were orally inoculated into Swiss Q, BALB/c, A/J, CBA/ CAH and nude (hypothymic) BALB/c mice, Fischer 344 and Wistar rats and specific pathogen free (SPF) hamsters. Twenty four human isolates of R. nana were cross-tested in flour beetles, Tribolium confusum. No adult worms were obtained from mice, rats or hamsters, even when immunosuppressed with cortisone acetate. Only one of the 24 samples developed to the cysticercoid stage in T. confusum; however, when inoculated into laboratory mice the cysticercoids failed to develop into adult worms. The large sample size used in this study, and the range of techniques employed for extraction and preparation of eggs provide a comprehensive test of the hypothesis that the human strain of R. nana is essentially non-infective to rodents.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Failure to infect laboratory rodent hosts with human isolates of Rodentolepis (= Hymenolepis) nana

et al. 2002

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“…This fact is of extreme relevance, taking into account that cestode infections induce very high levels of immunogenic responses in the parasitized animals (Lucas et al 1980, Ito, 1982). An interesting situation was observed in the inbred mice strains investigated: when the parasitism due to A. tetraptera reached high levels, those related to the infection with S. obvelata were low and vice-versa (Figs 5,8).…”

Section: Discussionmentioning

confidence: 99%

Helminth Parasites of Conventionally Maintained Laboratory Mice: II- Inbred Strains with an Adaptation of the Anal Swab Technique

Gonçalves

Pinto

Vicente

et al. 1998

Mem. Inst. Oswaldo Cruz

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Worm burdens recovered from inbred mice strains, namely C57Bl/6, C57Bl/10, CBA, BALB/c, DBA/ 2 and C3H/He, conventionally This approach intends to add new data on the helminth parasites of laboratory mice, since these investigations have arised a great interest and also, taking into account that these hosts as experimental animal models, are widely utilized in the evaluation of several biological parameters. Previously, besides the considered Swiss Webster mice, only two inbred strains (C57Bl/6, DBA/2) were investigated for the presence of helminths in Brazil (Pinto et al. 1994).Thus, the present findings ratify those reported for these strains, as well as complement the evaluation of worm burden in the other commonly referred inbred mice.The modification of the anal swab technique proposed herein aims to avoid unnecessary necropsies to investigate the spectrum of intestinal parasites in a rodent colony, since oxyurid eggs are of difficult detection during conventional stool examination procedures. MATERIALS AND METHODSTwo hundred and fifty Mus musculus (Linnaeus, 1758) inbred mice from two animal houses in the State of Rio de Janeiro, RJ, Brazil, were divided in two groups (A, B) according to their source. The suppliers were not identified by name due to ethical reasons. In group A, were included mice of the strains C57Bl/6, C57Bl/10, CBA, BALB/c, DBA/2 and C3H/He, with 25 animals each. The group B was represented by the strains C57Bl/6, CBA, BALB/c and C3H/He, with 25 mice each. Mice were male, 30 days old, weighting 20g each.Mice were sacrificed, helminths were recovered and processed for study as described elsewhere (Pinto et al. 1994).As for the swab anal procedures, the technique of Hall (1937) was modified and the device consists of a plastic rod (Fig. 1), with appropriate dimensions, for the examination of mice weighting at least 18g. One hundred and twelve mice were tested by this method. Animals are maintained in the proper position (Hofman's position), in order to avoid stress to the animals during the procedure. The rod is moistened in a 0.85% NaCl solution (Fig. 2) and after, introduced into the anus of the mouse, with carefully induced rotatory movements (Fig. 3), to avoid bleeding. The collected feces sample is transferred to a drop (20 µl) of saline physiological solution (0.85% NaCl) on a slide with a coverslip and examined under light microscope.

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“…Among cestodiosis, one case of T. crassiceps invasive cysticercosis has been described in an AIDS patient, 17 as well as one case of disseminated cestodiosis initially diagnosed as sparganosis in an immunodeficient patient with Hodgkin’s disease receiving chemotherapy 5 . The parasite observed in this latter case was secondarily reconsidered to be probably similar to H. nana 18,19 . The peripheral blood and sputum of immunodeficient patients may contain cysticercoids of H. nana , suggesting wide dissemination 20 .…”

mentioning

confidence: 82%