Abnormal Amino-Acid Concentrations in the Blood of Patients with Acquired Immunodeficiency Syndrome (AIDS) May Contribute to the Immunological Defect

Dröge, Wulf; Eck, Hans‐Peter; Näher, Helmut; Pekar, Ursula; Daniel, Volker

doi:10.1515/bchm3.1988.369.1.143

Cited by 83 publications

(27 citation statements)

References 8 publications

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“…Although it is possible that the isolated T cells can take up intact GSH, no evidence for this was found in studies on mouse peripheral lymphocytes in vivo (43), human lymphoid cell lines (31,41), and human peripheral lymphocytes (6,11 (45). Such patients have low levels of plasma cysteine and of GSH in blood mononuclear cells (46,47). The synthesis of GSH by peripheral lymphocytes seems to depend on transport of cysteine moieties.…”

Section: Resultsmentioning

confidence: 99%

Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphocytes stimulated via the CD2 and CD3 antigens.

Suthanthiran

Anderson

Sharma

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

335

176

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Regulation of proliferation of normal human T lymphocytes (T cells) by glutathione (GSH) was explored with T-cell activation models that-do not require accessory cell signals. L-Buthionine-(S,R)-sulfoximine (BSO), which inactivates y-glutamylcysteine synthetase and therefore inhibits GSH synthesis, inhibited proliferation elicited by monoclonal antibodies directed at cluster designation 2 (CD2) and CD3

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Section: Resultsmentioning

confidence: 99%

Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphocytes stimulated via the CD2 and CD3 antigens.

Suthanthiran

Anderson

Sharma

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

335

176

View full text Add to dashboard Cite

show abstract

“…Low plasma GSH has been described in liver disease (30) and in human immunodeficiency virus infection (31,32). Since plasma GSH is derived mainly from the liver and is governed by sinusoidal GSH transport, down-regulation of this polypeptide may contribute to the profound systemic effects, including brain and immune dysfunction, seen in liver disease and chronic viral infections such as human immunodeficiency virus.…”

Section: Discussionmentioning

confidence: 99%

Expression cloning of the cDNA for a polypeptide associated with rat hepatic sinusoidal reduced glutathione transport: characteristics and comparison with the canalicular transporter.

Fernández‐Checa

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

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Using the Xenopus oocyte expression system, we previously identified an -4-kb fraction of mRNA from rat liver that expresses sulfobromophthalein reduced glutathione S-conjugate (BSP-GSH)-insensitive and an -2.5-kb fraction expressing BSP-GSH-sensitive reduced glutathione (GSH) transport. From the former, a 4.05-kb cDNA was cloned and characterized as the putative rat canalicular GSH transporter. Starting with a cDNA library constructed from the -2.5-kb fraction, we have now isolated a single clone that leads to expression of a BSP-GSH-and cystathionine- bases. An '40-kDa protein is generated in in vitro translation of cRNA for RsGshT. Northern blot analysis revealed a single -2.8-kb transcript in rat and human liver with negligible hybridization signal in other organs. The abundance of mRNA for RsGshT did not increase with phenobarbital treatment. Cis-inhibition by BSP-GSH and trans-inhibition by cystathionine and lack of induction by phenobarbital are characteristic of sinusoidal GSH secretion and thus indicate that RsGshT either encodes the sinusoidal GSH transporter itself or a regulatory subunit of the transporter that determines its liver-specific activity.

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“…The Nef protein is mainly localized in the cytoplasm (Franchini et al, 1986), where reducing conditions are prevalent. It was shown that, especially in T cells of HIV-infected patients, the reduced glutathione (GSH) level is very low (Eck et al, 1989;Buhl et al, 1989;Roederer et al, 1991) because of the reduced cysteine and elevated glutamate levels in the blood (Eck et al, 1989;Droge et al, 1988). The intracellular GSH level in these cells is dependent on the uptake of cysteine or cystine, for which a different transport system exists.…”

Section: Discussionmentioning

confidence: 99%

Oligomerization of the Nef protein from human immunodeficiency virus (HIV) type 1

Kienzle

Freund

Kalbitzer

et al. 1993

European Journal of Biochemistry

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The nef genes, derived from two different human immunodeficiency-virus-type-1 (HIV-1) strains, were expressed in procaryotic cells (Escherichia coli) and in eucaryotic cells (insect cells infected with nef-containing baculovirus). The oligomerization of recombinant Nef protein was studied by NMR spectroscopy and immunoblotting under various experimental conditions. 1H-NMR spectroscopy shows that native folded protein has the tendency to polymerize under low-salt conditions. These oligomers become covalently linked by disulfide bonds after decreasing the reduction potential, a process which is fully reversible. Cross-linking studies with bis(sulfo-succinimidyl)suberate and alkylation with iodoacetic acid under non-reducing and reducing conditions document for the first time that Nef can also form homomeric structures including monomers, dimers, trimers and tetramers in cell lysates and intact cells. We found disulfide-linked as well as non-covalently associated oligomers. Since the Nef molecules are not exclusively found in the cytoplasm of HIV infected cells and since the reduced glutathione concentration in lymphocytes of virus infected persons is known to be unusually low, it might be possible that these Nef oligomers have a biological function in vivo as well

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Abnormal Amino-Acid Concentrations in the Blood of Patients with Acquired Immunodeficiency Syndrome (AIDS) May Contribute to the Immunological Defect

Cited by 83 publications

References 8 publications

Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphocytes stimulated via the CD2 and CD3 antigens.

Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphocytes stimulated via the CD2 and CD3 antigens.

Expression cloning of the cDNA for a polypeptide associated with rat hepatic sinusoidal reduced glutathione transport: characteristics and comparison with the canalicular transporter.

Oligomerization of the Nef protein from human immunodeficiency virus (HIV) type 1

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