Ablation of human colon carcinoma in nude mice by 131I-labeled monoclonal anti-carcinoembryonic antigen antibody F(ab')2 fragments.

Abstract

Pooled F(ab')2 fragments of three MAbs against distinct epitopes of carcinoembryonic antigen (CEA) were used for radioimmunotherapy of nude mice bearing a subcutaneous human colon carcinoma xenograft. 9-10 d after transplantation when tumor nodules were in exponential growth, 36 mice were

Carcinoembryonic antigen expression, antibody localisation and immunophotodetection of human colon cancer liver metastases in nude mice: A model for radioimmunotherapy

“…Such models have been used for radioimmunotherapy (RIT), radiotherapy and chemotherapy studies and have allowed direct measurement of tumour growth and remission (Cheung et al, 1986;Buchegger et al, 1989Buchegger et al, , 1995. However, S.C. tumours in mice might have different blood perfusion and tissue organisation compared with clinical primary and metastatic tumours.…”

The selection of antibodies for targeted therapy of small-cell lung cancer (SCLC) using a human tumour spheroid model to compare the uptake of cluster 1 and cluster w4 antibodies

“…Studies in animals with human tumour xenografts have demonstrated that the most favourable anti-tumour antibody uptake ratio is seen in small tumours (Rogers et al, 1986;Pedley et al, 1987), and this probably accounts for the good results of radioimmunotherapy in these animals (Cheung et al, 1986;Buchegger et al, 1989;Smith et al, 1991). In man radiolabelled antibodies have usually been given to treat large tumours, and although responses occur they are rarely sustained.…”

Les anticorps radiomarqués pour le traitement des cancers

“…Mais cette voie n'a pas été systématiquement suivie. La réduction de la taille du vecteur, en utilisant divers fragments d'anticorps, F(ab') 2 , Fab ou constructions à base de scFv (➜), n'a pas non plus apporté de résultat déci-sif [23]. La diffusion accrue des petites molécules dans l'organisme est contrebalancée par leur élimination plus rapide, ce qui est un avantage pour l'imagerie mais pas nécessairement pour la thérapie.…”