Ablation of FAM20C caused short root defects via suppressing the BMP signaling pathway in mice

Li, Lili; Liu, Peihong; Lv, Xuechao; Tian-Liang, YU; Jin, Xingai; Wang, Rui; Xie, Xiaoyan; Wang, Qingshan; Liu, Yingqun; Saiyin, Wuliji

doi:10.1007/s00056-022-00386-7

Cited by 5 publications

(5 citation statements)

References 34 publications

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“…This study conducted bicinchoninic Acid (BCA) assay (Wanlei Bio, Shenyang, China) for measuring sample protein content to normalize the loading size of samples. Proteins extracted in OSCC tissues were separated by SDS‐PAGE and analyzed through WB assay according to previous description 17,18 . Alpha FluorChem FC3 (Protein Simple, CA, USA) was employed for detecting protein bands, whereas ImageJ software was utilized for quantifying imaging data.…”

Section: Methodsmentioning

confidence: 99%

“…Proteins extracted in OSCC tissues were separated by SDS-PAGE and analyzed through WB assay according to previous description. 17,18 Alpha FluorChem FC3 (Protein Simple, CA, USA) was employed for detecting protein bands, whereas ImageJ software was utilized for quantifying imaging data. WB assay was conducted on proteins extracted in diverse samples.…”

Section: Protein Isolation and Western-blotting Assaymentioning

confidence: 99%

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Relationship of biglycan and decorin expression with clinicopathological features and prognosis in patients with oral squamous cell carcinoma

Liu

Wang

Piao

et al. 2022

J Oral Pathology Medicine

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Objective: This study focused on investigating relation between biglycan (BGN) and decorin (DCN) expression and prognostic outcome for oral squamous cell carcinoma (OSCC) cases. Material and Methods: BGN and DCN mRNA and protein expression was detected by qRT-PCR and Western-blotting (WB) assays from 31 OSCC samples as well as healthy samples. This work harvested 101 paraffin-embedded OSCC together with 30 healthy samples, and conducted immunohistochemical (IHC) staining for assessing pathological changes. Association of DCN with BGN within OSCC was explored by Spearman's analysis. Survival rate was explored by Kaplan-Meier (KM) approach. Multivariate analysis was conducted by Cox regression. Results: WB and qRT-PCR results showed BGN up-regulation (p < 0.001, p < 0.0001) whereas DCN down-regulation (p < 0.0001, p < 0.0001) with fresh OSCC tissues; the expression of BGN and DCN associated with the OSCC histopathological grade. IHC results suggested elevated BGN level (p < 0.0001) whereas DCN downregulation (p < 0.0001) with paraffin embedded OSCC tissues. The expression of BGN and DCN associated with histopathologic grades and tumor stage of OSCC.The result of Spearman's analysis demonstrated significant association between the expression of BGN and DCN in OSCC. Survival analysis revealed that patients with higher BGN/lower DCN level showed poor overall survival (OS) as well as tumorspecific survival (TSS). Multivariate analysis proved that BGN and DCN independently predicted the prognosis of OS and TSS. Conclusion:BGN and DCN expression levels can be adopted for predicting OSCC prognostic outcome.

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Section: Methodsmentioning

confidence: 99%

Section: Protein Isolation and Western-blotting Assaymentioning

confidence: 99%

Relationship of biglycan and decorin expression with clinicopathological features and prognosis in patients with oral squamous cell carcinoma

Liu

Wang

Piao

et al. 2022

J Oral Pathology Medicine

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“…Depletion of Fam20 c in mouse dental mesenchymal cells leads to reduced expression of Runx2 and Osx/Sp7 as well as downregulated transcription of Dmp1 and Dspp , indicating that FAM20C positively regulates odontoblastic differentiation ( Liu et al, 2018a ). Furthermore, in mouse models with ablation of Fam20 c, the expression of Dspp was reduced in odontoblasts from the root region, and the BMP signaling pathway was inhibited ( Li et al, 2022b ).…”

Section: Genetic Syndromes With Dentin Defects Caused By Impaired Odo...mentioning

confidence: 99%

The odontoblastic differentiation of dental mesenchymal stem cells: molecular regulation mechanism and related genetic syndromes

Pan,

Yang,

et al. 2023

Front. Cell Dev. Biol.

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Dental mesenchymal stem cells (DMSCs) are multipotent progenitor cells that can differentiate into multiple lineages including odontoblasts, osteoblasts, chondrocytes, neural cells, myocytes, cardiomyocytes, adipocytes, endothelial cells, melanocytes, and hepatocytes. Odontoblastic differentiation of DMSCs is pivotal in dentinogenesis, a delicate and dynamic process regulated at the molecular level by signaling pathways, transcription factors, and posttranscriptional and epigenetic regulation. Mutations or dysregulation of related genes may contribute to genetic diseases with dentin defects caused by impaired odontoblastic differentiation, including tricho-dento-osseous (TDO) syndrome, X-linked hypophosphatemic rickets (XLH), Raine syndrome (RS), hypophosphatasia (HPP), Schimke immuno-osseous dysplasia (SIOD), and Elsahy-Waters syndrome (EWS). Herein, recent progress in the molecular regulation of the odontoblastic differentiation of DMSCs is summarized. In addition, genetic syndromes associated with disorders of odontoblastic differentiation of DMSCs are discussed. An improved understanding of the molecular regulation and related genetic syndromes may help clinicians better understand the etiology and pathogenesis of dentin lesions in systematic diseases and identify novel treatment targets.

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“…Similarly, conditional disruption of Fam20c in both dental epithelium and dental mesenchyme leads to pronounced reduction in BMP-4 and phosphor-Smad1/5/9 levels, resulting in severe shortened roots and abnormal odontoblast differentiation. This disruption also correlates with decreased expression of downstream targets, including Osterix and Krüppel-like factor 4 (Klf4) ( Li et al, 2022 ). These findings provide additional evidence supporting the significance of BMP-4 in influencing odontoblast differentiation and root formation at precise concentrations.…”

Section: Roles Of Bmp Signaling In Tooth Root Developmentmentioning

confidence: 99%

BMP signaling in the development and regeneration of tooth roots: from mechanisms to applications

Liu,

Guo,

Shi

et al. 2023

Front. Cell Dev. Biol.

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Short root anomaly (SRA), along with caries, periodontitis, and trauma, can cause tooth loss, affecting the physical and mental health of patients. Dental implants have become widely utilized for tooth restoration; however, they exhibit certain limitations compared to natural tooth roots. Tissue engineering-mediated root regeneration offers a strategy to sustain a tooth with a physiologically more natural function by regenerating the bioengineered tooth root (bio-root) based on the bionic principle. While the process of tooth root development has been reported in previous studies, the specific molecular mechanisms remain unclear. The Bone Morphogenetic Proteins (BMPs) family is an essential factor regulating cellular activities and is involved in almost all tissue development. Recent studies have focused on exploring the mechanism of BMP signaling in tooth root development by using transgenic animal models and developing better tissue engineering strategies for bio-root regeneration. This article reviews the unique roles of BMP signaling in tooth root development and regeneration.

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Ablation of FAM20C caused short root defects via suppressing the BMP signaling pathway in mice

Cited by 5 publications

References 34 publications

Relationship of biglycan and decorin expression with clinicopathological features and prognosis in patients with oral squamous cell carcinoma

Relationship of biglycan and decorin expression with clinicopathological features and prognosis in patients with oral squamous cell carcinoma

The odontoblastic differentiation of dental mesenchymal stem cells: molecular regulation mechanism and related genetic syndromes

BMP signaling in the development and regeneration of tooth roots: from mechanisms to applications

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