Ablation for Benign Liver Tumors: Current Concepts and Limitations

Maruyama, Hitoshi; Tobari, Maki; Nagamatsu, Hiroaki; Yamaguchi, Tadashi; Shiina, Shuichiro

doi:10.14218/jcth.2022.00205

Cited by 1 publication

(1 citation statement)

References 46 publications

(88 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 2 Surgical resection is the traditional treatment for hepatic hemangiomas; however, it is traumatic and has many complications. 3 Recently, radiofrequency ablation (RFA) has been increasingly used in the clinical treatment of hepatic hemangiomas, showing advantages such as an obvious curative effect, minimal invasiveness, safety, and good application prospects.…”

Section: Introductionmentioning

confidence: 99%

Heme-Inducing Endothelial Pyroptosis Plays a Key Role in Radiofrequency Ablation of Hepatic Hemangioma Leading to Systemic Inflammatory Response Syndrome

Yao,

Kong,

et al. 2024

JIR

View full text Add to dashboard Cite

Purpose Systemic inflammatory response syndrome (SIRS) is a common complication of radiofrequency ablation (RFA) for hepatic hemangiomas. RFA can cause hemolytic reactions during hepatic hemangioma ablation. However, the mechanisms underlying RFA-induced SIRS remain unclear. Methods We established an orthotopic liver hemangioma model and performed radiofrequency ablation. The levels of interleukin (IL)-1β and IL-18 and the production of ROS were measured. The wet-to-dry lung ratio, inflammation score, and in vivo endothelial cell permeability were examined. GSDMD −/− mice were used to investigate the effect of heme-inducing SIRS. RNA sequencing (RNA-seq) was performed to identify the main pathways underlying heme-induced SIRS. Western blotting and immunoprecipitation were used to determine the changes and interactions of associated proteins. Results The levels of heme, IL-1β, and IL-18 were significantly increased after RFA. The wet-to-dry lung ratio increased in hepatic hemangiomas after RFA, indicating that SIRS occurred. Heme induced increased levels of IL-1β and IL-18, cell death, wet-to-dry lung radio, and inflammation score in vitro and in vivo, indicating that heme induced SIRS and pyroptosis. Furthermore, GSDMD participates in heme-induced SIRS in mice, and GSDMD deletion in mice reverses the effect of heme. Heme regulates NLRP3 activation through the NOX4/ROS/TXNIP-TRX pathway, and an N-acetyl-L-cysteine (NAC) or NOX4 inhibitor (GLX351322) reverses heme-induced SIRS. Conclusion Our findings suggest that heme induces endothelial cell pyroptosis and SIRS in mice and decreasing heme levels and ROS scavengers may prevent SIRS in hepatic hemangioma after RFA.

show abstract

Section: Introductionmentioning

confidence: 99%