Abiotic stress elements in in vitro potato (Solanum tuberosum L.) exposed to air-based and liquid-based ultrasound: A comparative transcriptomic assessment

Dobránszki, Judit; Hidvégi, Norbert; Gulyás, Andrea; Tóth, Bianka; Silva, Jaime A. Teixeira da

doi:10.1016/j.pbiomolbio.2020.09.001

Cited by 8 publications

(10 citation statements)

References 30 publications

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“…AA, essential for GSH regeneration, was 40 ng/ml in the 4-week-old plantlets. If a much stronger ultrasound stress was applied ( Dobránszki et al., 2020 ), as presented in this work, it could badly disturb the membrane integrity according to the literature ( Rokhina et al., 2009 ), but we have got unexpected plant responses. The concentration of small molecule antioxidants was not increased compared with the control plants.…”

Section: Discussionmentioning

confidence: 66%

Ultrasonication affects the melatonin and auxin levels and the antioxidant system in potato in vitro

et al. 2022

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Melatonin is an ancient hormone whose physiological effects have been extensively studied in animals and human. We now know that it also plays a prominent role in the growth and development of plants. In our present experiment, the relationship between endogenous melatonin and the antioxidant system was investigated in potato plant grown in vitro. Changes in redox homeostasis under ultrasound stress were examined. The concentration of small molecule antioxidants and enzymes of the three-level antioxidant pathway was measured. ELISA method was used to determine the melatonin levels in plant tissues at each growth stage (0 h, 24 h, 48 h, 1 week, and 4 weeks after subculturing the explants) both in control and ultrasound-treated plants. Ultrasound stress activated the three-level defense system and decreased the endogenous melatonin levels. Melatonin was able to provide protection against membrane damage caused by drastic ultrasound treatment. Melatonin at the heart of the redox network is a key component regulating various biochemical, cellular, and physiological responses. It has a dual role, as it is able to act both as a growth regulator and an antioxidant. A close relationship was evidenced between the plant hormone indole-3-acetic acid and melatonin and ascorbic acid.

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Section: Discussionmentioning

confidence: 66%

Ultrasonication affects the melatonin and auxin levels and the antioxidant system in potato in vitro

et al. 2022

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“…Recent discoveries have confirmed that plants can sense, generate, and emit sound waves, which play a role in plant adaptation to the environment [6][7][8]. Physical signals, including acoustic signals, can quickly propagate at lower energy costs, enabling plants to adapt to environmental changes more swiftly and efficiently compared to chemical responses [7][8][9]. Acoustic waves are capable of transmitting energy and environmental information to plants, leading to changes in their growth and development [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, it influences various biological and physiological processes in plants, such as seed germination, the cell cycle, cell proliferation, shoot and root growth, callus development, signal transduction systems, enzyme and hormone activity, and gene expression [10,13,13,[22][23][24][25]. Recent publications [9,24,26] and collaborative studies [13] have highlighted the ability of ultrasound to alter potato plants in response to stress, impact mRNA transcription, and enhance in vitro growth and development.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasound Application in Potato Cultivation: Potential for Enhanced Yield and Sustainable Agriculture

Pszczółkowski,

Sawicka

2023

Sustainability

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Ultrasounds, characterized by high-frequency air vibrations exceeding 20 kHz, have traditionally found applications in medicine and the food industry, primarily for analyzing chemical composition and food product structure. They also have potential uses in agriculture, particularly in potato cultivation. Objective: The aim of this study was to assess the potential to increase yields of selected potato varieties through the use of ultrasound in agricultural practices. The research findings were derived from a field experiment conducted on Luvisols between 2015 and 2017 in central-eastern Poland. The field experience was designed using a randomized complete-block split-plot layout with three replications. The primary factor included eight potato cultivars representing various maturity groups. The second-order factors included two cultivation management practices: (A) ultrasound application as a pre-plant treatment, and (B) a control group without ultrasound application (sonication). The study assessed potato tuber yield and its structural characteristics. Results: Tuber yield was influenced by the chosen cultivation practices, as well as the variations in responses among potato cultivars to environmental factors and pre-plant treatments. Conclusion: The research findings suggest that the use of ultrasounds in agricultural practices holds promise as a valuable tool for promoting sustainable agriculture, increasing potato cultivation productivity, and fostering environmentally friendly production methods.

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“…On the other hand, the free radicals produced by hydrolysis can interact with amino acid residues and affect the activity of enzymes (29, 31). There has been some evidence of the effect of ultrasound on gene expression in plant, for instance, changes of mRNA transcription in potato exposed to ultrasound were observed (42)(43)(44). Noteworthily, a prior study in Arabidopsis exposed to sonication discovered transcriptomic changes, and changes in reactive oxygen species (ROS) scavenging ability, as well as in phytohormonal metabolism (45).…”

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confidence: 99%

Transcriptomic and physiological analysis reveals the possible mechanism of ultrasound inhibiting strawberry (Fragaria × ananassa Duch.) postharvest softening

Zhang

Jiang

et al. 2022

Front. Nutr.

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Ultrasound effectively inhibited strawberry softening but the mechanism was not clear. In this study, physical data including firmness, soluble pectin (SP) contents, pectin esterase (PE), polygalacturonase (PG) activity and transcriptome sequencing data were analyzed to explore the mechanism of strawberry response to ultrasonic treatment. After 24 days storage, the firmness reduction rate and soluble contents (SP) increased rate of the strawberry treated with ultrasound (25 kHz, 0.15 W/cm2) for 3 min decreased 41.70 and 63.12% compared with the control, respectively. While the PG and PE enzyme activities of ultrasound-treated strawberries were significantly lower than control after storage for 18 days. A total of 1,905 diferentially expressed genes (DEGs) were identified between ultrasound-treated and control, with 714 genes upregulated and 1,254 genes downregulated, including 56 genes in reactive oxygen species (ROS), auxin (AUX), ethylene (ETH) and jasmonic acid (JA) signaling pathways. At 0 h, 15 genes including LOX, JMT, ARP, SKP, SAUR, IAA, ARF, and LAX were significantly upregulated compared with the control group, which means reactive oxygen specie, auxin, ethylene and jasmonic acid-mediated signaling pathway respond to ultrasound immediately. ERF109, ERF110, and ACS1_2_6 downregulated before 2 days storage indicated ethylene signaling pathway was inhibited, while after 2 days, 9 genes including ERF027, ERF109, and ERF110 were significantly upregulated indicating that the response of the ethylene signaling pathway was lagging. Therefore, in strawberry ultrasound enhanced ROS scavenging and activated JA biosynthesis, which acts as a signal for delaying the activation of ET signaling pathway, thus suppressing the activity of pectin-degrading enzymes PE and PG, and ultimately inhibiting postharvest softening.

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Abiotic stress elements in in vitro potato (Solanum tuberosum L.) exposed to air-based and liquid-based ultrasound: A comparative transcriptomic assessment

Cited by 8 publications

References 30 publications

Ultrasonication affects the melatonin and auxin levels and the antioxidant system in potato in vitro

Ultrasonication affects the melatonin and auxin levels and the antioxidant system in potato in vitro

Ultrasound Application in Potato Cultivation: Potential for Enhanced Yield and Sustainable Agriculture

Transcriptomic and physiological analysis reveals the possible mechanism of ultrasound inhibiting strawberry (Fragaria × ananassa Duch.) postharvest softening

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