Abiotic Stress Diagnosis via Laser Induced Chlorophyll Fluorescence Analysis in Plants for Biofuel

The current study was designed to identify the stage for the diagnosis of disease before visible symptoms appeared. Fluorescence spectroscopy has been employed to identify disease signatures for its early diagnosis in rice plant leaves. Bacterial leaf blight (BLB) diseased and healthy leaf samples were collected from the rice fields in September, 2017 which were then used to record spectra using an excitation wavelength at 410 nm. The spectral range of emission was set from 420 to 800 nm which covers the blue-green and the chlorophyll bands. It was found that diseased leaves have a narrower 'chlorophyll a' band than healthy ones, and furthermore, that the emission band at 730 nm was either declined or depleted in the sample with high infection symptoms. In contrast, the blue-green region was observed to increase due to the emergence of disease. As the band intensity of chlorophyll decreases during infection, this decrease in chlorophyll content and increase in the blue-green spectral region could provide a new approach for predicting BLB at an early stage. The important finding was that the chlorophyll degradation and rise in the blue-green region take place in leaves with BLB or during BLB infection. Principal component analysis has been applied to spectral data which successfully separated diseased samples from healthy ones even with very small spectral variations.

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Synchronous fluorescence spectroscopy for early diagnosis of citrus canker in citrus species

Atta¹,

Saleem²,

Ali³

et al. 2019

Laser Phys.

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Citrus canker is among the most destructive diseases of citrus crops that cause huge economic losses owing to a reduction in citrus production and at the same time threaten the marketability of citrus [1,2]. This disease is prevalent in most citrus-growing countries around the globe [3]. Citrus canker is caused by the bacterium Xanthomonas axonopodis pv. citri (Syn. X. citri pv. citri). However, the bacteria associated with the disease have been divided into different pathotypes based on a host range and symptomology. These include pathotypes; A (X. axonopodis pv. citri), B and C (X. axonopodis pv. aurantifolii), and E (X. axonopodis pv. citrumelo) [4]. Citrus canker symptoms on leaves, stems and fruits consist of Laser Physics

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Abiotic Stress Diagnosis via Laser Induced Chlorophyll Fluorescence Analysis in Plants for Biofuel

Cited by 3 publications

References 38 publications

The use of laser induced chlorophyll fluorescence (LIF) as a fast and non‑destructive method to investigate water deficit in Arabidopsis

The use of laser induced chlorophyll fluorescence (LIF) as a fast and non‑destructive method to investigate water deficit in Arabidopsis

Chlorophyll as a biomarker for early disease diagnosis

Synchronous fluorescence spectroscopy for early diagnosis of citrus canker in citrus species

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