Ability of plasmid DNA complexed with histidinylated lPEI and lPEI to cross in vitro lung and muscle vascular endothelial barriers

Gomez, Jean-Pierre; Pichon, Chantal; Midoux, Patrick

doi:10.1016/j.gene.2013.03.055

Cited by 10 publications

(6 citation statements)

References 56 publications

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“…MCEC are a frequently used in vitro cell system 11,12,[16][17][18][19] . Though they showed endothelial cell-like behavior in in vitro assays, MCEC apparently lost key endothelial cell characteristics in their gene expression program.…”

Section: Discussionmentioning

confidence: 99%

Gene expression in immortalized versus primary isolated cardiac endothelial cells

Deng

Pollmeier

Zhou

et al. 2020

Sci Rep

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Endothelial cells take pivotal roles in the heart and the vascular system and their differentiation, subspecification and function is determined by gene expression. A stable, in vitro cardiac endothelial cell line could provide high cell numbers as needed for many epigenetic analyses and facilitate the understanding of molecular mechanisms involved in endothelial cell biology. To test their suitability for transcriptomic or epigenetic studies, we compared the transcriptome of cultured immortalized mouse cardiac endothelial cells (MCEC) to primary cardiac endothelial cells (pEC). Whole transcriptome comparison of MCEC and pEC showed a correlation of 0.75-0.77. Interestingly, correlation of gene expression declined in endothelial cell-typical genes. In MCEC, we found a broad downregulation of genes that are highly expressed in pEC, including well-described markers of endothelial cell differentiation. Accordingly, systematic analysis revealed a downregulation of genes associated with typical endothelial cell functions in MCEC, while genes related to mitotic cell cycle were upregulated when compared to pEC. In conclusion, the findings from this study suggest that primary cardiac endothelial cells should preferably be used for genome-wide transcriptome or epigenome studies. The suitability of in vitro cell lines for experiments investigating single genes or signaling pathways should be carefully validated before use.

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Section: Discussionmentioning

confidence: 99%

Gene expression in immortalized versus primary isolated cardiac endothelial cells

Deng

Pollmeier

Zhou

et al. 2020

Sci Rep

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“…Building on the proton sponge hypothesis, histidines or other imidazole derivatives with a p K a of around 6 have been incorporated into polymers such as polylysine, PEI, or dendrimers to increase their endosomal buffer capacity. Various reports demonstrate the beneficial effects of these buffering units on transfection efficiency …”

Section: Cationic Core Polymersmentioning

confidence: 99%

Nucleic Acid Therapeutics Using Polyplexes: A Journey of 50 Years (and Beyond)

2015

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“…As reported in the literature, branched PEI is a well‐known cytotoxic molecule in most cell types related to its direct interaction with plasma membrane molecules, and forms a precipitation on the cell surface 32 . Safer alternatives of PEI could be designed for in vivo administrations because of its beneficial activities on a wide range of advanced biological applications, including nonviral gene, 26 DNA, nucleic acid, and drug delivery, 33 transfection efficiency, 13 and especially significant antimicrobial activity 12 . Polyplex forms of PEI containing DNA, lipids or different biomolecules reduce the toxicity of branched PEI thanks to the lower cationic charge density of the polyplex form 14 .…”

Section: Resultsmentioning

confidence: 99%

“…12 However, the high toxicity of PEI limits its direct use to inhibit bactericidal, fungal or viral growth, especially in human contact application areas. 12 To overcome this less biocompatibility effect, preparation of complex form PEI with various biomacromolecules such as DNA, 13 lipids, 14 and phenolic compounds. 15 Sahiner and Demirci 16 reported that synthesis of betainized PEI microgels was the most feasible approach in design of more compatible PEI-based material.…”

Section: Introductionmentioning

confidence: 99%

Degradable, biocompatible, and antibacterial polygalacturonic acid/polyethyleneimine polyplex particles

Suner

2023

Polymers for Advanced Techs

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Anionic polygalacturonic acid (PGA), a prebiotic for healthy microbiota, and cationic polyethyleneimine (PEI), a strong antimicrobial polymer against harmful infections, were ionically crosslinked to prepare biocompatible and antibacterial polygalacturonic acid/polyethyleneimine (PGA/PEI) polyplex particles. The PGA/PEI particles were spherical in shape and had a wrinkled surface with a micron size range of particles from 9 to 0.6 μm. The PGA/PEI polyplex particle was degradable under more acidic and basic conditions but was highly stable between pH 3 and 7. The prepared PGA/PEI polyplex particles are found compatible for intravenous applications with hemolysis rate of less than 1% and almost 100% blood clotting index values up to 1 mg/mL concentration. Similarly, no significant toxicity was found on L929 fibroblast cells for 24 h incubation time in the presence of 1 mg/mL concentration of the PGA/PEI polyplex particles. As an antibacterial material, the PGA/PEI polyplex particles totally prevented the growth of Gram‐negative Escherichia coli and Gram‐positive Staphylococcus aureus. Due to the antibacterial, biocompatible, blood compatibility, and degradability, the PGA/PEI polyplex particles can be utilized in wide range of application such as particularly wound dressing, cosmetic and health care products, and drug delivery applications.

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Ability of plasmid DNA complexed with histidinylated lPEI and lPEI to cross in vitro lung and muscle vascular endothelial barriers

Cited by 10 publications

References 56 publications

Gene expression in immortalized versus primary isolated cardiac endothelial cells

Gene expression in immortalized versus primary isolated cardiac endothelial cells

Nucleic Acid Therapeutics Using Polyplexes: A Journey of 50 Years (and Beyond)

Degradable, biocompatible, and antibacterial polygalacturonic acid/polyethyleneimine polyplex particles

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