Ability of Pit and Fissure Sealant-containing Amorphous Calcium Phosphate to inhibit Enamel Demineralization

Abstract

Aim: To evaluate the effect of amorphous calcium phosphate (ACP)-containing pit and fissure sealant on inhibition of enamel demineralization in vitro.Materials and methods: Enamel specimens (n = 75) were prepared using freshly extracted noncarious human third molars. Box-shaped cavities (8 × 2 × 2 mm) on the buccal or lingual surfaces were prepared and restored with resin-based sealant (Concise™), ACP-containing sealant (Aegis®) or fluoride-containing sealant (Conseal-F™). The samples were acid challenged in a demineralizing solution of 50 mmol/l lactic acid at pH 5.0 for 4 days. The change in enamel microhardness (ASuH) was calculated. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey’s post hoc test.Results: The mean SuH0 (±SD) (in Vicker’s unit) prior to the acid challenge was: Concise™ (318.83 ± 33.86), Aegis® (331.03 ± 21.52), Conseal-F™ (310.12 ± 34.31). Following the acid challenge, the values dropped in all groups and ASuH (±SD) values were 269.17 ± 47.49, 151.39 ± 23.96 and 175.79 ± 32.39 respectively.Conclusion: The ACP-containing pit and fissure sealant has the potential to inhibit enamel demineralization.How to cite this article: Zawaideh FI, Owais AI, Kawaja W. Ability of pit and fissure sealant-containing amorphous calcium phosphate to inhibit enamel demineralization. Int J Clin Pediatr Dent 2016;9(1):10-14.

Ca-P spots modified zirconia by liquid precursor infiltration and the effect on osteoblast-like cell responses

“…This was because the ACP on the zirconia ceramics regains the hydroxyl in the hydrothermal treatment, which was similar to the research of Ha 29) . On the other hand, it confirmed the ACP is a precursor to the formation of HAP, which is the final, stable product in the precipitation of the calcium and phosphate ions from neutral or basic solutions 30) . 32) .…”

“…On the other hand, ACP-containing bioactive materials stimulate mineral growth by increasing the calcium and phosphate concentrations within the lesion, especially in acidic environment, thereby shifting the thermodynamic driving forces of the solution toward the formation of apatite 30) . As the dissolution experiments results showed, Ca-P spots modified zirconia exhibited the higher Ca 2+ and PO4 3− release before hydrothermal treatment compared to after hydrothermal treatment (p<0.05).…”

The Applications and Modifications of Casein Phosphopeptide-Amorphous Calcium Phosphate: An Update

“…Yassaei et al [74] reported the highest release of calcium ions and increased efficiency of Er:YAG laser to decrease demineralization when combined with CPP ACP products. A study by Hajizadeh et al [75] also found that the combined effect of Er:YAG laser with CPP-ACP and fluoride significantly increased dentin hardness and Er:YAG laser alone did not lead to increased dentin resistance to caries.…”

Ion release and recharge from a fissure sealant containing amorphous calcium phosphate

“…However, several factors may influence the long-term success of FS, such as oral biofilms containing S. mutans, which has esterase activity that hydrolyzes the ester bonds in materials containing methacrylate [3,4], or enamel demineralization in some parts of the teeth [5]. For this reason, particles such as fluoride or amorphous calcium phosphate (ACP) are added to FS materials to improve the preventive properties of sealants by increasing enamel remineralization and decreasing subsequent demineralization and secondary caries [6][7][8][9].…”