Ability of peritoneal exudate cells to trigger various mechanisms of erythroid differentiation at different terms after massive blood loss in mice

Abstract

Thirty minutes after massive blood loss, peritoneal cells gained the ability to trigger reparative erythropoiesis in the bone marrow of normal syngeneic recipients. This was manifested in proliferation of oxyphilic erythroblasts and activation of the reserve pathway of erythroid differentiation 4 days after cell transplantation. The maximum transfer capacity of peritoneal cells was observed 4 h after blood loss, but 4 days later they only initiated mitoses in oxyphilic erythroblasts.

Various aspects of the involvement of peritoneal exudate cells in the regulation of apoptosis

“…Previous experiments with adoptive transfer of PC from animals with massive hemorrhage to healthy syngeneic recipients showed that changes in the mitotic index of erythroblasts and percentage of cells in the bone marrow is related to activity of PC in response to hemorrhage [1], but not to administration of the culture medium.…”

“…Series I was performed by a scheme resulting in maximum stimulation of bone marrow erythropoiesis after transplantation of PC from anemic donors [1]. Transplantation of 0.5 ml suspension of PC was followed by a significant increase in MCHC and MCH compared to the intact control (Table 1).…”

“…Experiments were performed on male inbred CBA mice weighing 23-27 g. Experimental conditions were selected taking into account published data [1]. The ability of PC to stimulate bone marrow erythropoiesis was maximum 2-18 h after blood withdrawal (2.5% body weight).…”

“…Stimulation of bone marrow erythropoiesis and increase in the number of blood reticulocytes were observed starting from the 4th day after adoptive transfer of PC. The isolation of PC (5 h after hemorrhage) and intraperitoneal transplantation of cells to syngeneic recipients were performed using culture medium 199 on glutamine-free Hanks solution (pH 7.2; M. P. Chumakov Institute of Poliomyelitis and Viral Diseases, Russian Academy of Medical Sciences) [1].…”

“…Over the first hours after external hemorrhage, peritoneal cells (PC) in vivo gain the ability to induce intensive proliferation of bone marrow erythroid cells and modify cell differentiation. PC are characterized by extreme potency in stimulating erythropoiesis under conditions of massive hemorrhage; adoptive transfer of PC into the abdominal cavity of intact syngeneic recipients causes an imbalance of erythropoiesis and the appearance of signs of "emergency" erythropoiesis typical of mice with hemorrhage [1]. Apart from the proliferative response of bone marrow erythroid precursors, activation of hemoglobin (Hb) synthesis is an important compensatory mechanism during hemorrhage.…”