Ability of male Queensland fruit flies to inhibit receptivity in multiple mates, and the associated recovery of accessory glands

Radhakrishnan, Preethi; Taylor, Phillip W.

doi:10.1016/j.jinsphys.2007.10.014

Cited by 49 publications

(57 citation statements)

References 25 publications

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“…In the Queensland fruitfly, males transfer a spermatophore to the female during mating that inhibits the female receptivity. The accessory glands decrease in size after mating, and between 5.5h and 11h are needed for a full replenishment and recovery of the initial size of the glands in order to induce an inhibition in the next female (Radhakrishnan and Taylor, 2008).…”

Section: Discussionmentioning

confidence: 99%

Switching attraction to inhibition: mating-induced reversed role of sex pheromone in an insect

Barrozo¹,

Gadenne²,

Anton³

2010

Journal of Experimental Biology

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SUMMARYIn the moth, Agrotis ipsilon, newly mated males cease to be attracted to the female-produced sex pheromone, preventing them from re-mating until the next night, by which time they would have refilled their reproductive glands for a potential new ejaculate. The behavioural plasticity is accompanied by a decrease in neuron sensitivity within the primary olfactory centre, the antennal lobe (AL). However, it was not clear whether the lack of the sexually guided behaviour results from the absence of sex pheromone detection in the ALs, or if they ignore it in spite of detection, or if the sex pheromone itself inhibits attraction behaviour after mating. To test these hypotheses, we performed behavioural tests and intracellular recordings of AL neurons to non-pheromonal odours (flower volatiles), different doses of sex pheromone and their mixtures in virgin and newly mated males. Our results show that, although the behavioural and AL neuron responses to flower volatiles alone were similar between virgin and mated males, the behavioural response of mated males to flower odours was inhibited by adding pheromone doses above the detection threshold of central neurons. Moreover, we show that the sex pheromone becomes inhibitory by differential central processing: below a specific threshold, it is not detected within the AL; above this threshold, it becomes inhibitory, preventing newly mated males from responding even to plant odours. Mated male moths have thus evolved a strategy based on transient odour-selective central processing, which allows them to avoid the risk-taking, energy-consuming search for females and delay re-mating until the next night for a potential new ejaculate.

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Section: Discussionmentioning

confidence: 99%

Switching attraction to inhibition: mating-induced reversed role of sex pheromone in an insect

Barrozo¹,

Gadenne²,

Anton³

2010

Journal of Experimental Biology

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“…Previous studies of D. melanogaster demonstrated that mating induces transcription and translation of SFP genes (Bertram et al 1992; DiBenedetto et al 1990; Herndon et al 1997; Monsma et al 1990; Schmidt et al 1985; Simmerl et al 1995; Styger 1992), yet ours is among the first to demonstrate that males appear to fully replenish their SFP levels within 3 days of sexual inactivity (see also Coleman et al 1995). SFP replenishment also appears to occur in other Diptera: Male stalk-eyed flies ( Cyrtodiopsis dalmanni ) and Queensland fruit flies ( Bactrocera tryoni ) restore their accessory glands (the main site of SFP synthesis) to virgin size within ~24 h after mating (Rogers et al 2005; Radhakrishnan and Taylor 2008). Interestingly, SFP replenishment does not appear to occur in all Diptera.…”

Section: Discussionmentioning

confidence: 99%

“…Evidence from several species suggests that, like sperm (Wedell et al 2002), SFPs are a limited resource (Andersson et al 2004; Bissoondath and Wiklund 1996; Hihara 1981; Linklater et al 2007; Perez-Staples et al 2008; Radhakrishnan and Taylor 2008; Savalli and Fox 1999; Smith et al 1990; Torres-Vila and Jennions 2005; Vahed 2007; Wigby et al 2009). In some species, repeated matings by males reduces post-mating responses of their successive mates, suggesting that SFPs become depleted (e.g., Drosophila melanogaster : Hihara 1981; Anastrepha obliqua : Perez-Staples et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Seminal fluid protein depletion and replenishment in the fruit fly, Drosophila melanogaster: an ELISA-based method for tracking individual ejaculates

Sirot

Buehner

Fiumera

et al. 2009

Behav Ecol Sociobiol

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In many species, seminal fluid proteins (SFPs) affect female post-mating behavioral patterns, including sperm storage, egg laying, feeding, and remating. Yet, few studies have investigated the patterns of allocation, depletion, and replenishment of SFPs in male animals, despite the importance of these proteins to male and female reproductive success. To investigate such SFP dynamics, it is necessary to have a sensitive method for quantifying SFP levels in males and mated females. We developed such a method by adapting the enzyme-linked immunosorbent assay (ELISA) using anti-SFP antibodies. Here, we first use two Drosophila melanogaster SFPs (ovulin and sex peptide) to demonstrate that ELISAs provide accurate measures of SFP levels. We find that, consistent with previous data from Western blotting or immunofluorescence studies, levels of both ovulin and sex peptide decline in the mated female with time since mating, but they do so at different rates. We then use ELISAs to show that males become depleted of SFPs with repeated matings, but that previously mated males are able to transfer “virgin” levels of SFPs after 3 days of sexual inactivity. Finally, we demonstrate that ELISAs can detect SFPs from wild-caught D. melanogaster males and, thus, potentially can be used to track mating patterns in the wild. This method of measuring SFP dynamics can be used in a wide range of species to address questions related to male reproductive investment, female mating history, and variation in female post-mating behavioral changes.

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“…In support of this hypothesis, virgin B. tryoni females injected with male RT extracts experience diminished sexual receptivity and a shorter copulation duration when subsequently mated, similar to behaviors seen in previously mated females (94). That male AG size decreases after mating in B. tryoni suggests that this tissue is a major site of SFP synthesis (95). …”

Section: Function Of Identified Sfpsmentioning

confidence: 99%

Insect Seminal Fluid Proteins: Identification and Function

Avila

Sirot

LaFlamme

et al. 2011

Annu. Rev. Entomol.

785

844

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Seminal fluid proteins (SFPs) produced in reproductive tract tissues of male insects and transferred to females during mating induce numerous physiological and behavioral post-mating changes in females. These changes include decreasing receptivity to re-mating, affecting sperm storage parameters, increasing egg production, modulating sperm competition, feeding behaviors, and mating plug formation. In addition, SFPs also have anti-microbial functions and induce expression of anti-microbial peptides in at least some insects. Here, we review recent identification of insect SFPs and discuss the multiple roles these proteins play in the post-mating processes of female insects.

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Ability of male Queensland fruit flies to inhibit receptivity in multiple mates, and the associated recovery of accessory glands

Cited by 49 publications

References 25 publications

Switching attraction to inhibition: mating-induced reversed role of sex pheromone in an insect

Switching attraction to inhibition: mating-induced reversed role of sex pheromone in an insect

Seminal fluid protein depletion and replenishment in the fruit fly, Drosophila melanogaster: an ELISA-based method for tracking individual ejaculates

Insect Seminal Fluid Proteins: Identification and Function

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