Ability of Infants to Taste PTC: Its Application in Cases of Doubtful Paternity

Hm, Cardullo; Le, Holt

doi:10.3181/00379727-76-18569

Cited by 10 publications

(7 citation statements)

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“…Family and twin studies suggest this trait is inherited as a Mendelian recessive, with two alleles typically represented as T and t, with T representing the 'tasting' allele and t the 'non-tasting' allele (Blakeslee 1931, Snyder 1931, Blakeslee 1932, Levit and Soboleva 1935, Lee 1937, Rife 1938, Hogben 1946, Matsunaga and Tsuji 1957, Merton 1958, Pons 1960, Martin 1975, Rao and Morton 1977, Forrai and Bankovi 1984, Whissell-Buechy 1990b. The evidence for a genetic component underlying the PTC tasting ability is so strong that it was once used in paternity tests before DNA markers were available (Cardullo and Holt 1951). The ability to taste PTC is listed as a genetic trait (McKusick 1995), (MIM No.…”

Section: Introductionmentioning

confidence: 99%

The genetics of phenylthiocarbamide perception

Guo

Reed

2001

Annals of Human Biology

262

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The ability to taste the bitter compound phenylthiocarbamide (PTC) and related chemicals is bimodal, and all human populations tested to date contain some people who can and some people who cannot taste PTC. Why this trait has been maintained in the population is uncertain but this polymorphism may influence food selection, nutritional status or thyroid metabolism. The gene product that gives rise to this phenotype is unknown, and its characterization would provide insight into the mechanism of bitter taste perception. Although this trait is often considered a simple Mendelian trait, i.e. one gene two alleles, a recent linkage study found a major locus on chromosome 5p15 and evidence for an additional locus on chromosome 7. The development of methods to identify these genes will provide a good stepping-stone between single-gene disorders and polygenic traits.

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Section: Introductionmentioning

confidence: 99%

The genetics of phenylthiocarbamide perception

Guo

Reed

2001

Annals of Human Biology

262

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“…Besides its importance in genetic and anthropological studies, PTC taste sensitivity has been shown to be important in food selection, which may affect individual metabolism and physiology [33]. It was previously used in paternity testing before the advent of DNA markers [34].…”

Section: Discussionmentioning

confidence: 99%

Distribution of sensory taste thresholds for phenylthiocarbamide (PTC) taste ability in North Indian Muslim populations

Hussain

Shah

Afzal

2013

Egyptian Journal of Medical Human Genetics

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The ability to taste Phenylthiocarbamide (PTC), a bitter organic compound has been described as a bimodal autosomal trait in both genetic and anthropological studies. This study is based on the ability of a person to taste PTC. The present study reports the threshold distribution of PTC taste sensitivity among some Muslim populations of North India, as till now no detailed information is available. A survey was conducted among healthy individuals within the age range of 10-45 years who were randomly selected from among six populations viz; Syed, Sheikh, Pathan, Ansari, Qureshi and Saifi. The PTC tasting ability was measured using a serial dilution method of Harris and Kalmus. A bimodal distribution was observed from the graphs plotted for the PTC threshold distribution. The mean PTC threshold values (±SD) of the male and female individuals were calculated as 8.12 ± 0.21 and 8.39 ± 0.20, respectively. The threshold values among the six populations ranged from 7.71 to 8.81 among males, 7.44-9.04 among females and 7.86-8.91 as combined. The results found that females show a higher mean threshold value than males, though of no statistical significance. This type of study will provide brief information on the distribution of PTC sensory thresholds among some Muslim populations of North India. This study has some physiological relevance to highlight the adaptability of endogamous groups to behavioral traits in the same place.Ó 2013 Production and hosting by Elsevier B.V. on behalf of Ain Shams University.

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“…10 The evidence for a genetic basis for variation in PTC sensitivity was so consistent that PTC sensitivity was used as a paternity test before the invention of molecular genetic tests. 11 In 2003, Un-kyung Kim and colleagues determined that a single gene on chromosome 7q encoding a member of the TAS2R bitter-taste receptor family is the locus for PTC sensitivity. They concluded that taster status was conferred by three coding single-nucleotide polymorphisms in this gene, which produced distinct haplotypes.…”

Section: The Human Ptc Genementioning

confidence: 99%

“…Snyder ultimately concluded that nontaster status was conferred by a recessive allele at a single locus, but the exact locus remained a mystery 10 . The evidence for a genetic basis for variation in PTC sensitivity was so consistent that PTC sensitivity was used as a paternity test before the invention of molecular genetic tests 11 …”

Section: Introductionmentioning

confidence: 99%

Using the Integrated Genome Viewer to reveal amplicon‐derived polymorphism enriched at the phenylthiocarbamide locus in the teaching lab

Brenner¹,

Scheid²,

DeGrazia

et al. 2021

Biochem Molecular Bio Educ

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Due to its distinct phenotype and relatively simple inheritance pattern, the phenylthiocarbamide (PTC) loci is frequently utilized in teaching laboratories to demonstrate genetic concepts such as Mendelian inheritance and population genetics. We have developed a next-generation sequencing and bioinformatics approach to analyze the PTC gene locus to reveal single nucleotide polymorphism (SNP) variation at nucleotide position 785 that predicts tasting ability in humans. Here students purify DNA from their own cheek cells, perform polymerase chain reaction (PCR) amplification of the PTC gene followed by cleaved amplified polymorphic sequence (CAPS) testing. Students perform a second PCR on the PTC loci using high-fidelity Taq to create bar-coded amplicons for next-generation sequencing on the Ion Torrent Personal Genome Machine. Bioinformatic verification reveals polymorphic variation by aligning the entire class PTC PCR fragment sequence to the human gene using Bowtie2 and visualizing the results in the Integrated Genome Viewer. This exercise presents a learning opportunity for students to use next-generation sequencing to predict their own PTC taste sensitivity phenotype coupled with the standard CAPS method. This approach brings the PTC teaching method into the genomics era.

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Ability of Infants to Taste PTC: Its Application in Cases of Doubtful Paternity

Cited by 10 publications

References 0 publications

The genetics of phenylthiocarbamide perception

The genetics of phenylthiocarbamide perception

Distribution of sensory taste thresholds for phenylthiocarbamide (PTC) taste ability in North Indian Muslim populations

Using the Integrated Genome Viewer to reveal amplicon‐derived polymorphism enriched at the phenylthiocarbamide locus in the teaching lab

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