Ability of Different Growth Indicators to Detect Salt Tolerance of Advanced Spring Wheat Lines Grown in Real Field Conditions

Abstract

Plant growth indicators (GIs) are important for evaluating how different genotypes respond to normal and stress conditions separately. They consider both the morphological and physiological components of plants between two successive growth stages. Despite their significance, GIs are not commonly used as screening criteria for detecting salt tolerance of genotypes. In this study, 36 recombinant inbred lines (RILs) along with four genotypes differing in their salt tolerance were grown under normal and 150 mM NaCl in a two-year field trial. The performance and salt tolerance of these germplasms were assessed through various GIs. The analysis of variance showed highly significant variation between salinity levels, genotypes, and their interaction for all GIs and other traits in each year and combined data for two years, with a few exceptions. All traits and GIs were significantly reduced by salinity stress, except for relative growth rate (RGR), net assimilation rate (NAR), and specific leaf weight (SLW), which increased under salinity conditions. Traits and GIs were more correlated with each other under salinity than under normal conditions. Principal component analysis organized traits and GIs into three main groups under both conditions, with RGR, NAR, and specific leaf area (SLA) closely associated with grain yield (GY) and harvest index, while leaf area duration (LAD) was closely associated with green leaf area (GLA), plant dry weight (PDW), and leaf area index (LAI). A hierarchical clustering heatmap based on GIs and traits organized germplasms into three and four groups under normal and salinity conditions, respectively. Based on the values of traits and GIs for each group, the germplasms varied from high- to low-performing groups under normal conditions and from salt-tolerant to salt-sensitive groups under salinity conditions. RGR, NAR, and LAD were important factors determining genotypic variation in GY of high- and low-performing groups, while all GIs, except leaf area duration (LAR), were major factors describing genotypic variation in GY of salt-tolerant and salt-sensitive groups. In conclusion, different GIs that reveal the relationship between the morphological and physiological components of genotypes could serve as valuable selection criteria for evaluating the performance of genotypes under normal conditions and their salt tolerance under salinity stress conditions.