Ability of Allium cepa L. root tips and Tradescantia pallida var. purpurea in N-nitrosodiethylamine genotoxicity and mutagenicity evaluation

Abstract

N -nitroso compounds, such as N -nitrosodiethylamine (NDEA), can be formed by the reaction of secundary amines with nitrosating agents, and are suspected to be involved in tumors in humans. NDEA has been considered a weak carcinogen in genotoxic assays probably due to the inefficient nitrosamine activation system that is used and/or to the efficient repair system. In this work, we evaluated the sensibility of Allium cepa L. root tips and Tradescantia stamen hair mutation assay (Trad-SH) using Tradescantia pallida var. purpurea for NDEA (0.1; 0.5; 5 and 25mM) genotoxicity and mutagenicity induction. Allium cepa L. was treated with different NDEA concentrations for 3h, for 3 consecutive days, including negative control (distilled water) and positive control maleic hydrazide (MH 30μg/mL). After treatment, the roots were hydrolyzed, squashed, and the mitotic index (MI) and cytological abnormalities were scored. The results revealed a cytostatic effect of NDEA (0.5 and 5mM), showing a significant reduction in the MI. Chromosome stickiness suggests a NDEA toxic effect. T. pallida purpurea did not respond to mutagens with a dosedependent pattern. In conclusion, our study indicates that the root tips of Allium cepa L. have sensibility to detect NDEA genotoxicity, but not for Trad-SH test.

Allium cepa and Tradescantia pallida bioassays to evaluate effects of the insecticide imidacloprid

“…Another plant used in the present study was T. pallida. However, few studies have used this species to evaluate the effects of pesticides (Raino et al, 2010) because it is most commonly used to evaluate environmental samples (Savóia et al, 2009;Crispim et al, 2012;Pereira et al, 2013). The results involving T. pallida indicate significant dose-dependent increases in the frequencies of MN in the groups exposed to the three IMI concentrations.…”

Genotoxicity of Polycyclic Aromatic Hydrocarbons and Nitro-Derived in Respirable Airborne Particulate Matter Collected from Urban Areas of Rio de Janeiro (Brazil)

“…The roots were exposed to treatment with organic extract and the controls for 24 h. Afterwards, the roots were removed from the bulbs and hydrolyzed with HCl 1N : acetic acid 45% (1 : 9) at 50°C for 5 min. The hydrolyzed root tips were squashed and stained with 2% aceto-orcein [24, 25]. Cytological abnormalities like micronuclei (MCN), bridges, breaks, and laggards were analyzed.…”

Study on tissue compatibility and genotoxicity of a radiation sterilized disposable burette transfusion apparatus

“…The mutagenicity and genotoxicity evaluation, using more than one test system, is often pointed out [12]. In our study we combined two tests to check for the genotoxicity and mutagenicity of the radiation sterilized disposable burette transfusion apparatus.The tests of gene mutation and chromosome aberration which directly reflect mutagenicity of toxic chemicals are reliable methods to evaluate mutagenicity of toxic chemicals [9,13,14].…”