Ability of Agrobacterium tumefaciens and A. rhizogenes strains, inability of A. vitis and A. rubi strains to adapt to salt-insufficient environment, and taxonomic significance of a simple salt requirement test in the pathogenic Agrobacterium species

Tanaka, Katsuyuki; Arafat, Hussam Hassan; Urbanczyk, Henryk; Yamamoto, Sachiko; Moriguchi, Kazuki; Sawada, Hiroyuki; Suzuki, K.

doi:10.2323/jgam.55.35

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…Protocols for the ferric ammonium citrate test and 3-ketolactose production test followed general methods, as described by Moore et al (39). Growth in media containing a high concentration of NaCl was tested using liquid and solid LB (50). Vitamin requirement was determined by repeated-streak inoculation of a thin bacterial suspension (two toothpick-scratches of a solid culture in 1 ml water) to a synthetic medium for Agrobacterium (AB) agar consisting of minerals (1ϫ AB salts) (51), 0.5% glucose, and 1.5% agar and by subsequent cultivation for 4 days.…”

Section: Methodsmentioning

confidence: 99%

The Presence of the Hairy-Root-Disease-Inducing (Ri) Plasmid in Wheat Endophytic Rhizobia Explains a Pathogen Reservoir Function of Healthy Resistant Plants

Kang

Maeshige

Okamoto

et al. 2020

Appl Environ Microbiol

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Rhizobium radiobacter species complex (biovar 1 Agrobacterium) has been known for the crown-gall and hairy-root disease causative pathogens. Strains within this complex were also found as endophytes from many plant species with no symptom. The aim of this study is to reveal the endophyte variation of this complex and how these endophytic strains differ from pathogenic strains. In this study we devised a simple but effective screening method by exploiting the high resolution power of mass spectrometry. We screened endophyte isolates from young wheat and barley plants, which are resistant to the diseases, and identified seven isolates from wheat as members in the R. radiobacter species complex. Through further analyses, we assigned five strains to the genomovar (genomic group) G1 and two strains to G7 in the species. Notably, these two genomovar groups harbor many known pathogenic strains. In fact, the two G7 endophyte strains showed pathogenicity on tobacco, as well as the virulence prerequisites including a 200-kbp Ri plasmid. All of the five G1 strains possessed a 500-kbp plasmid, which is present in well-known crown-gall pathogens. These data strongly suggest a role of healthy wheat plants as a reservoir for pathogenic strains of this species. IMPORTANCE Crown gall and hairy root diseases exhibit very wide host-plant ranges that cover gymnosperm and dicot plants. Rhizobium radiobacter species complex is the representative species that harbor causative agents of the two diseases. Recently, a number of endophyte isolates from many plant species have been assigned to this species complex. We isolated seven endophyte strains belonging to the species complex from wheat plants, and revealed their genomovar affiliations and plasmid profile. The significance of this study is the finding of the genomovar correlation between the endophytes and the known pathogens, the presence of a virulence ability in two of the seven endophyte strains, and the high ratio of the pathogenic strains in the endophyte strains. This study therefore provides convincing evidences that could unravel the mechanism to maintain pathogenic agents of this species and to deliver them to susceptible plants sporadically.

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Section: Methodsmentioning

confidence: 99%

The Presence of the Hairy-Root-Disease-Inducing (Ri) Plasmid in Wheat Endophytic Rhizobia Explains a Pathogen Reservoir Function of Healthy Resistant Plants

Kang

Maeshige

Okamoto

et al. 2020

Appl Environ Microbiol

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“…and Ro-Ag-13) were cultured at 28 o C in IFO medium 702 (0.5% [wt/vol] polypeptone, 0.2% yeast extract, 0.01% MgSO4, pH 7.0) (Tanaka et al, 2009). When necessary, antibiotics were added to the media at the following final concentrations: kanamycin (50 µg/ml), neomycin (50 µg/ml), rifampicin (40 µg/ml) and nalidixic acid (30 µg/ml).…”

Section: Ae0078712)mentioning

confidence: 99%

Development of a reinforced Ti-eviction plasmid useful for construction of Ti plasmid-free Agrobacterium strains

Yamamoto

Suzuki

2012

Journal of Microbiological Methods

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“…The seed-borne strain CCGM7 was characterized by screening for genes that allow the bacteria to survive in seeds (Peralta et al, 2016). To extend this research, we tested nodules formed by CCGM7 (or a strain from soil, CFNEI73) and isolated two strains, named CCGM10 and CCGM11, capable of growing in LB medium, a phenotype reported for certain strains of agrobacteria (Tanaka et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Genomic Comparison of Agrobacterium pusense Strains Isolated from Bean Nodules

et al. 2016

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Ability of Agrobacterium tumefaciens and A. rhizogenes strains, inability of A. vitis and A. rubi strains to adapt to salt-insufficient environment, and taxonomic significance of a simple salt requirement test in the pathogenic Agrobacterium species

Cited by 5 publications

References 22 publications

The Presence of the Hairy-Root-Disease-Inducing (Ri) Plasmid in Wheat Endophytic Rhizobia Explains a Pathogen Reservoir Function of Healthy Resistant Plants

The Presence of the Hairy-Root-Disease-Inducing (Ri) Plasmid in Wheat Endophytic Rhizobia Explains a Pathogen Reservoir Function of Healthy Resistant Plants

Development of a reinforced Ti-eviction plasmid useful for construction of Ti plasmid-free Agrobacterium strains

Genomic Comparison of Agrobacterium pusense Strains Isolated from Bean Nodules

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