ABI3 mediated repression of RAV1 gene expression promotes efficient dehydration stress response in Arabidopsis thaliana

Sengupta, Sourabh; Ray, Anagh; Mandal, Drishti; Chaudhuri, Ronita Nag

doi:10.1016/j.bbagrm.2020.194582

Cited by 11 publications

(41 citation statements)

References 69 publications

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“…The overexpression of Arabidopsis RAV1 increased the sensitivity of transgenic plants to salt and drought stress ( Fu et al. 2014 , Sengupta et al. 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Chromatin Accessibility and Gene Expression upon Cold Stress Reveals that the RAV1 Transcription Factor Functions in Cold Response inVitis Amurensis

Ren

Wang

et al. 2021

Plant and Cell Physiology

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Cold tolerance is regulated by a variety of transcription factors (TFs) and their target genes. Except for the well-characterized C-repeat binding factors (CBFs)-dependent transcriptional cascade, the mechanisms of cold tolerance mediated by other transcriptional regulatory networks are still largely unknown. Here we used the assay for transposase-accessible chromatin with sequencing (ATAC-seq) and RNA-seq to identify cold responsive TFs in Vitis amurensis, a grape species with high cold hardiness. A number of 9 TFs, including CBF4, RAV1 and ERF104, were identified after cold treatment. Weighted gene co-expression network analysis (WGCNA) and gene ontology (GO) analysis revealed that these TFs may regulate cold response through different pathways. As a prime candidate TF, overexpression of VaRAV1 in grape cells improved its cold tolerance. The transgenic cells exhibited low electrolyte leakage and malondialdehyde (MDA) content and high peroxidase (POD) activity. Moreover, the TF gene TCP8 and a gene involving in homogalacturonan biosynthesis were found to be regulated by VaRAV1, suggesting that the contribution of VaRAV1 to cold tolerance may be achieved by enhancing stability of cell membrane and regulating the expression of target genes involved in plant cell wall composition. Our work provides novel insights into plant response to cold stress and demonstrates the utility of ATAC-seq and RNA-seq for rapid identification of TFs in response to cold stress in grapevine. The VaRAV1 may play an important role in adaption to cold stress.

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“…The overexpression of Arabidopsis RAV1 increased the sensitivity of transgenic plants to salt and drought stress ( Fu et al. 2014 , Sengupta et al. 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Chromatin Accessibility and Gene Expression upon Cold Stress Reveals that the RAV1 Transcription Factor Functions in Cold Response inVitis Amurensis

Ren

Wang

et al. 2021

Plant and Cell Physiology

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“…Pteridophytes showed Motif-10 only in GolS and PLC promoters. Interestingly, under stress ABI3 TFs are reported to downregulate expression of developmental specific RAV1 TFs, thus maintaining a fine balance between stress and development (Sengupta et al 2020). Hence, presence of CAREs for both ABI3VP1 and RAV1 in Motifs 4 and 10 is quite significant as it points out as a switch to regulate the available resources towards development or halt it when the plants are exposed to stressful environment.…”

Section: Regulatory Signatures For Initiating Stressresponsive Inositol Metabolismmentioning

confidence: 99%

Regulation of stress-induced inositol metabolism in plants: a phylogenetic search for conserved cis elements

Basak

Majumder

2021

J. Plant Biochem. Biotechnol.

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Inositol and its' metabolic derivatives such as galactinol, raffinose, methylated inositols etc. are well known significant osmoprotectants produced in response to various abiotic stresses in plants. The present work focuses on the importance of phylogenetic analysis in search of a presumptive 'master switch' involved in inducing a set of stress-responsive coregulated genes in the pathways involved in inositol synthesis and its further metabolism. It can be reasonably assumed that the entire network of genes induced under a particular stress is governed by a few common stress-responsive transcription factor binding sites or cis elements in their respective promoters. A phylogenetic analysis of such regulatory regions of the coregulated genes participating in entire inositol metabolic pathway throughout evolution, can form a novel approach in recognizing the conserved stress-regulatory signature(s) or 'master switch(es)' regulating this important pathway under the stress impact.

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“…The cDNA obtained was used for q-PCR based analysis with primers specific to AUX1, GH3.7, GH3.15, and ABI5 as per the method followed by Sengupta et.al., 2020. 21…”

Section: Methodsmentioning

confidence: 99%

“…[17][18][19] Earlier we have shown that ABI3 mediates dehydration stress response in Arabidopsis thaliana by regulating expression of an array of downstream genes. 20,21 Furthermore, ABI3 auto-activates its own expression during dehydration stress response primarily through the Sph/RYcis element present in its promoter region. 22 In this work we show that in absence of ABI3, indirect de-novo root regeneration is compromised in Arabidopsis thaliana callus cells, indicating the role of ABI3 in root regeneration.…”

Section: Introductionmentioning

confidence: 99%

ABI3 plays a role in de-novo root regeneration from Arabidopsis thaliana callus cells

Sengupta

Chaudhuri

2020

Plant Signaling & Behavior

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Developmental plasticity and the ability to regenerate organs during the life cycle are a signature feature of plant system. De novo organogenesis is a common mode of plant regeneration and may occur directly from the explant or indirectly via callus formation. It is now evident that callus formation occurs through the root development pathway. In fact, callus cells behave like a group of root primordium cells that are under the control of exogenous auxin. Presence or absence of auxin decides the subsequent fate of these cells. While in presence of external supplementation of auxin they are maintained as root primordia cells, absence of exogenous auxin induces the callus cells into patterning, differentiation and finally root emergence. Here we show that in absence of functional ABI3, a prominent member of the B3 superfamily of transcription factors, root regeneration is compromised in Arabidopsis callus cells. In culture medium free of any exogenous hormone supplementation, while adventitious root emergence and growth was prominently observed in wild type cells, no such features were observed in abi3-6 cells. Expression of auxin-responsive AUX1 and GH3 genes was significantly reduced in abi3-6 cells, indicating that auxin levels or distribution may be altered in absence of ABI3.

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ABI3 mediated repression of RAV1 gene expression promotes efficient dehydration stress response in Arabidopsis thaliana

Cited by 11 publications

References 69 publications

Characterization of Chromatin Accessibility and Gene Expression upon Cold Stress Reveals that the RAV1 Transcription Factor Functions in Cold Response inVitis Amurensis

Characterization of Chromatin Accessibility and Gene Expression upon Cold Stress Reveals that the RAV1 Transcription Factor Functions in Cold Response inVitis Amurensis

Regulation of stress-induced inositol metabolism in plants: a phylogenetic search for conserved cis elements

ABI3 plays a role in de-novo root regeneration from Arabidopsis thaliana callus cells

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