Aberration-free dynamic focusing with a multichannel micromachined membrane deformable mirror

Luan, Zhu; Sun, Pang-Chen; Fainman, Yeshaiahu

doi:10.1364/ao.38.005350

Cited by 22 publications

(10 citation statements)

References 7 publications

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“…In such techniques, scanning different axial planes requires the objective lens to be physically moved with an actuator, such as a stepper motor or a nanopositioner, a process which is temporally limited by the combined inertia of objective lens and actuator. Several methods have been developed to quickly change the focal position, and thus allow for axial scanning, including deformable mirrors 25 , variable focus liquid-filled lenses 26 , and simultaneous spatial and temporal focusing 27,28 . However, none have the ability to simultaneously scan in the lateral dimension, and none have been demonstrated to functionally image parameters of neuronal activity.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity

et al. 2008

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The dynamic ability of neuronal dendrites to shape and integrate synaptic responses is the hallmark of information processing in the brain. Effectively studying this phenomenon requires concurrent measurements at multiple sites on live neurons. Significant progress has been made by optical imaging systems which combine confocal and multiphoton microscopy with inertia-free laser scanning. However, all systems developed to date restrict fast imaging to two dimensions. This severely limits the extent to which neurons can be studied, since they represent complex threedimensional (3D) structures. Here we present a novel imaging system that utilizes a unique arrangement of acousto-optic deflectors to steer a focused ultra-fast laser beam to arbitrary locations in 3D space without moving the objective lens. As we demonstrate, this highly versatile randomaccess multiphoton microscope supports functional imaging of complex 3D cellular structures such as neuronal dendrites or neural populations at acquisition rates on the order of tens of kilohertz.

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Section: Introductionmentioning

confidence: 99%

Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity

et al. 2008

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“…Other methods for fast focusing, such as deformable mirrors (Zhu et al 1999), fluid-filled lenses (Zhang et al 2003), and electro-optic lenses (Shibaguchi and Funato 1992), have all been shown and are possibilities for offering fast 3D scanning when combined with either galvanometers or other 2D scanning methods. However, to our knowledge, none has yet been shown in two-photon imaging systems.…”

Section: D Scanning Systemsmentioning

confidence: 99%

High-Speed Two-Photon Imaging

Reddy¹,

Saggau²

2013

Cold Spring Harb Protoc

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The small size of neuronal dendrites and spines combined with the high speed of neurophysiological signals, such as transients in membrane potential or ion concentration, necessitates that any functional study of these structures uses recording methods with both high spatial and high temporal resolutions. In this regard, conventional two-photon microscopy, in combination with fluorescent indicators sensitive to physiological parameters, has proved to be only a partial solution by providing near-diffraction-limited spatial resolution even when imaging structures deep inside light-scattering tissue. This is because the relatively slow beam-scanning methods used in most conventional two-photon microscopes severely limit the extent to which functional data can be recorded. Here, we detail developments to create high-speed two-photon imaging systems that overcome this limitation and discuss important considerations that must be taken into account when attempting to construct such systems.

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“…Compared to the liquid lenses optical zoom system, the deformable mirror zoom system has a distinct advantage of being free of chromatic aberrations. [23][24][25] So we employ two deformable mirrors to be variable elements to realize an optical zoom in our system. The principle part of the system consists of a Cassegrain and a Cassegrain-inverse configuration.…”

Section: Active Optical System For Anymentioning

confidence: 99%

Active optical system for any field angle zoom

2011

Opt. Eng

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We have theoretically proposed a new type of active optical zoom system. It can change the magnification of an imaging system at any field angle, namely, it can change the effective focal length at a limited region of interest and simultaneously maintain high resolution. It is comprised of two static aspheric mirrors and two deformable mirrors; furthermore, by changing the curvature of mirrors the focal length can be adjusted from − 399 to − 558.6 mm. The system reduces the bandwidth of data transmission and may find potential applications in high speed photography. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).Subject terms: any field angle; deformable mirror; active zoom system; optical systems design.Paper 110523RR

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Aberration-free dynamic focusing with a multichannel micromachined membrane deformable mirror

Cited by 22 publications

References 7 publications

Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity

Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity

High-Speed Two-Photon Imaging

Active optical system for any field angle zoom

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