Aberrant upregulation of 14-3-3ơ expression serves as an inferior prognostic biomarker for gastric cancer

Abstract

Background14-3-3ơ is an intracellular, phosphoserine binding protein and proposed to be involved in tumorigenesis. However, the expression dynamics of 14-3-3ơ and its clinicopathological/prognostic significance in human tumors are still controversial.MethodsThe method of immunohistochemistry (IHC) and Western blot were utilized to examine the protein expression of 14-3-3ơ in gastric cancer and paired normal adjacent gastric mucosal tissues. Receive operating characteristic (ROC) curve analysis was employed to determine a cutoff score for 14-3-3ơ expression in a training set (n = 66). For validation, the ROC-derived cutoff score was subjected to analysis of the association of 14-3-3ơ expression with patient outcome and clinical characteristics in a testing set (n = 86) and overall patients (n = 152).ResultsThe expression frequency and expression levels of 14-3-3ơ were significantly higher in gastric cancer than in normal gastric mucosal tissues. Correlation analysis demonstrated that high expression of 14-3-3ơ in gastric cancer was significantly correlated with clinical stage and tumor invasion. Furthermore, in the testing set and overall patients, Kaplan-Meier analysis showed that elevated 14-3-3ơ expression predicted poorer overall survival (OS) and progression-free survival (PFS). Importantly, high 14-3-3ơ expression was also associated with shortened survival time in stage III and stage IV gastric cancer patients. Multivariate analyses revealed that 14-3-3ơ expression was an independent prognostic parameter in gastric cancer.ConclusionsThese findings provide evidence that high expression of 14-3-3ơ may be important in the tumor progression and servers as an independent molecular marker for poor prognosis of gastric cancer. Thus, overexpression of 14-3-3ơ identifies patients at high risk and is a novel therapeutic molecular target for this tumor.

Decreased Muc5AC expression is associated with poor prognosis in gastric cancer

“…The genes were associated with cell adhesion and regulation of cell proliferation (Table ). The genes for fibroblast growth factor binding protein 1 ( FGFBP1 ), stratifin ( SFN ), melanoma inhibitory activity ( MIA ), Muc4 ( MUC4 ), macrophage stimulating 1 receptor ( MST1R ), and catenin (cadherin‐associated protein) beta 1 ( CTNNB1 ) are reportedly involved in cancer progression.…”

“…cDNA microarrays of fresh gastric cancer tissue used in our study revealed differential expression of genes related to metastasis and invasion in tissues with high (≥25%) and low (<25%) levels of Muc5AC expression. Among the altered genes, FGFBP1 , SFN , MIA , MUC4 , MST1R and CTNNB1 are reportedly involved in cancer progression. These results indicate the possible genes underlying Muc5AC‐associated cancer invasion mechanisms.…”

Aurora-A Identifies Early Recurrence and Poor Prognosis and Promises a Potential Therapeutic Target in Triple Negative Breast Cancer

“…The immunohistochemistry (IHC) assay was performed as previously described [30]. The TMAs slides were deparaffinized in xylene, rehydrated through graded alcohol, and subsequently immersed in 3% hydrogen peroxide for 10 min to block endogenous peroxidase activity.…”

Clinical Significance of Increased Guanine Nucleotide Exchange Factor Vav3 Expression in Human Gastric Cancer

“…Thus, molecular pathology may be helpful to understand the disease pathogenesis and provide useful prognostic molecular markers. Potential biologic prognostic markers include overexpression of 14-3-3o, VEGF, mesothelin, EGF receptor, and elevated plasma level of osteopontin (6)(7)(8)(9)(10).…”