Aberrant TdT Expression in Germ Cell Neoplasia—A Pitfall in Differential Diagnosis With Lymphoma?

Gayhart, Matthew; Smith, Steven C.; Wiles, Austin; Richard, Hope

doi:10.1093/ajcp/aqy090.080

Cited by 4 publications

(2 citation statements)

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“…In order to utilize TdT as a biomarker in clinical practice and target for drug discovery, accurate and robust detection of TdT is essential. The currently used standard methods for TdT detection are based on immunological assays or gel electrophoreses [10–13] . These methods are time‐consuming, labor intensive, expensive, complex and do not allow for real‐time quantification of enzyme activity.…”

Section: Introductionmentioning

confidence: 99%

“…The currently used standard methods for TdT detection are based on immunological assays or gel electrophoreses. [10][11][12][13] These methods are time-consuming, labor intensive, expensive, complex and do not allow for real-time quantification of enzyme activity. Due to the high medical interest, several new detection methods of TdT have been developed over the last decade, to address some of these obstacles.…”

Section: Introductionmentioning

confidence: 99%

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A Quenched Size‐Expanded Nucleotide Reports Activity of the Leukemia Biomarker Terminal Deoxynucleotidyl Transferase (TdT)**

Pals

Velema

2023

Angewandte Chemie

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Terminal deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that plays an essential role in the human adaptive immune system and is upregulated in several types of leukemia. It has therefore gained interest as a leukemia biomarker and potential therapeutic target. Herein, we describe a FRET-quenched fluorogenic probe based on a sizeexpanded deoxyadenosine that reports directly on TdT enzymatic activity. The probe enables real-time detection of primer extension and de novo synthesis activity of TdT and displays selectivity over other polymerase and phosphatase enzymes. Importantly, TdT activity and its response to treatment with a promiscuous polymerase inhibitor could be monitored in human Tlymphocyte cell extract and Jurkat cells using a simple fluorescence assay. Finally, employing the probe in a high-throughput assay resulted in the identification of a non-nucleoside TdT inhibitor.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Quenched Size‐Expanded Nucleotide Reports Activity of the Leukemia Biomarker Terminal Deoxynucleotidyl Transferase (TdT)**

Pals

Velema

2023

Angewandte Chemie

View full text Add to dashboard Cite

show abstract

A Quenched Size‐Expanded Nucleotide Reports Activity of the Leukemia Biomarker Terminal Deoxynucleotidyl Transferase (TdT)**

Pals

Velema

2023

Angew Chem Int Ed

View full text Add to dashboard Cite

Terminal deoxynucleotidyl Transferase (TdT) is a template‐independent DNA polymerase that plays an essential role in the human adaptive immune system and is upregulated in several types of leukemia. It has therefore gained interest as a leukemia biomarker and potential therapeutic target. Herein, we describe a FRET‐quenched fluorogenic probe based on a size‐expanded deoxyadenosine that reports directly on TdT enzymatic activity. The probe enables real‐time detection of primer extension and de novo synthesis activity of TdT and displays selectivity over other polymerase and phosphatase enzymes. Importantly, TdT activity and its response to treatment with a promiscuous polymerase inhibitor could be monitored in human T‐lymphocyte cell extract and Jurkat cells using a simple fluorescence assay. Finally, employing the probe in a high‐throughput assay resulted in the identification of a non‐nucleoside TdT inhibitor.

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Photocatalytic colorimetry and fluorescence dual-signal biosensor for instant determination of terminal deoxynucleotidyl transferase

He,

Chen,

Lian

et al. 2024

Sensors and Actuators B: Chemical

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Aberrant TdT Expression in Germ Cell Neoplasia—A Pitfall in Differential Diagnosis With Lymphoma?

Cited by 4 publications

References 0 publications

A Quenched Size‐Expanded Nucleotide Reports Activity of the Leukemia Biomarker Terminal Deoxynucleotidyl Transferase (TdT)**

A Quenched Size‐Expanded Nucleotide Reports Activity of the Leukemia Biomarker Terminal Deoxynucleotidyl Transferase (TdT)**

A Quenched Size‐Expanded Nucleotide Reports Activity of the Leukemia Biomarker Terminal Deoxynucleotidyl Transferase (TdT)**

Photocatalytic colorimetry and fluorescence dual-signal biosensor for instant determination of terminal deoxynucleotidyl transferase

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