Aberrant T‐Cell Receptor Signalling of Interferon‐γ‐ and Tumour Necrosis Factor‐α‐Producing Cytotoxic CD8⁺ Vδ1/Vβ16 T Cells in a Patient with Chronic Neutropenia

Abstract

We previously found that the peripheral blood (PB) mononuclear cells (MCs) (PBMCs) of a patient with chronic neutropenia contained an expanded population of cytotoxic CD8 þ

Characterization of human αβTCR repertoire and discovery of D-D fusion in TCRβ chains

“…Some groups report that TRDV1 can rearrange with some TRAJ gene segments (Bank et al, 1992; Bank et al, 2003; Castelli et al, 1992; Miossec, 1990; Miossec et al, 1991; Ueno et al, 2003), while others believed that it just appears as a normal TRDV gene segment (Lefranc, 2001) that can only be engaged in TRD chain formation. In this study, our approaches could detect transcripts only if they contained the TRAC segment.…”

“…However, some TRDV gene segments form joints with TRAJ gene and can therefore be used to synthesize TRA. Furthermore, some specific CD8 + T cell clones with TRDV1 + TRA recognize and kill HIV-infected target cells (Ueno et al, 2003), while other TRDV1 + CD8 + T cell clones are believed to contribute to chronic neutropenia (Bank et al, 1992; Bank et al, 2003). In total, five out of eight TRDV genes, including TRDV4 to TRDV8, have been classified into this group (Lefranc, 2001), and their names are designated in the form of TRAV/DV plus the corresponding sequential numbers.…”

Vγ9+ γδ T cells in systemic sclerosis patients are numerically and functionally preserved and induce fibroblast apoptosis

“…PBMC were isolated by Ficoll Hypaque (Sigma, St. Louis, MO, USA) density centrifugation as previously described (Bank et al 2003). MC were collected from the interface after centrifugation, washed  3 with RPMI 1640 medium (Gibco, Invitrogen) and resuspended in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco BRL, UK), 2 mM glutamine and penicillin-streptomycin solution (100 mg/ml) (Ta'assiot Biologiot, Beit Haemek, Israel).…”

Pathophysiologic mechanisms and management of neutropenia associated with large granular lymphocytic leukemia

“…Furthermore, CD34 + cells from patients with AA, who have consistently elevated levels of TNF-a and IFN-g, upregulate Fas expression on CD34 + hematopoietic progenitors [71]. Interestingly, lymphocytes from some patients with T-LGL leukemia spontaneously secrete IFN-g and can also be induced to produce TNF-a [72]. Based on these observations, it has been proposed that in T-LGL leukemia, TNF-a and IFN-g, together with the overproduction of FasL, may induce Fas-mediated apoptosis of granulocytic progenitors [5,14,28].…”