Aberrant P-cadherin expression is associated to aggressive feline mammary carcinomas

Figueira, Ana Catarina; Gomes, Catarina; Oliveira, Joana Tavares de; Vilhena, Hugo; Carvalheira, J.; Matos, Augusto J. F. de; Dias‐Pereira, Patrícia; Gärtner, Fátima

doi:10.1186/s12917-014-0270-z

Cited by 7 publications

(12 citation statements)

References 72 publications

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“…Furthermore, they observed a reduced expression of E-cadherin in the tumors expressing N-cadherin [ 86 ]. Figueira et al [ 119 ] identified a direct relationship between the expression of another cadherin, P-cadherin and the aggressive biological behavior of feline mammary carcinomas. Together, these results suggest that N-cadherin and P-cadherin may be promising biomarkers of poor prognosis in FMCs.…”

Section: Cat Critical Cancer Genes: From Gene Expression Patterns mentioning

confidence: 99%

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Cat Mammary Tumors: Genetic Models for the Human Counterpart

Adega

Borges

Chaves

2016

Veterinary Sciences

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The records are not clear, but Man has been sheltering the cat inside his home for over 12,000 years. The close proximity of this companion animal, however, goes beyond sharing the same roof; it extends to the great similarity found at the cellular and molecular levels. Researchers have found a striking resemblance between subtypes of feline mammary tumors and their human counterparts that goes from the genes to the pathways involved in cancer initiation and progression. Spontaneous cat mammary pre-invasive intraepithelial lesions (hyperplasias and neoplasias) and malignant lesions seem to share a wide repertoire of molecular features with their human counterparts. In the present review, we tried to compile all the genetics aspects published (i.e., chromosomal alterations, critical cancer genes and their expression) regarding cat mammary tumors, which support the cat as a valuable alternative in vitro cell and animal model (i.e., cat mammary cell lines and the spontaneous tumors, respectively), but also to present a critical point of view of some of the issues that really need to be investigated in future research.

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Section: Cat Critical Cancer Genes: From Gene Expression Patterns mentioning

confidence: 99%

“…Together, these results suggest that N-cadherin and P-cadherin may be promising biomarkers of poor prognosis in FMCs. Moreover, Figueira et al [ 119 ], suggest that the aberrant expression of P-cadherin is in fact a stronger marker for aggressiveness than the underexpression of E-cadherin.…”

Section: Cat Critical Cancer Genes: From Gene Expression Patterns mentioning

confidence: 99%

Cat Mammary Tumors: Genetic Models for the Human Counterpart

Adega

Borges

Chaves

2016

Veterinary Sciences

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“…Mammary tumours are among the most frequent neoplasias in female cats . Approximately, 80% to 90% are malignant, and most of these present an aggressive behaviour and a poor prognosis . Reported survival times from diagnosis of queens with malignant mammary tumours vary significantly from a few days to several years …”

Section: Introductionmentioning

confidence: 99%

“…1 Approximately, 80% to 90% are malignant, and most of these present an aggressive behaviour and a poor prognosis. [2][3][4] Reported survival times from diagnosis of queens with malignant mammary tumours vary significantly from a few days to several years. 5,6 Several studies characterized feline mammary tumours and evaluated related prognostic factors, most of them on clinical, histopathological and molecular features of these neoplasias.…”

Section: Introductionmentioning

confidence: 99%

Acute phase proteins and biomarkers of oxidative status in feline spontaneous malignant mammary tumours

Vilhena

Tvarijonaviciute

Cerón

et al. 2019

Vet Comparative Oncology

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Acute phase proteins (APP) and biomarkers of oxidative status change in human and canine mammary tumours, however, they have not been studied in feline mammary tumours. The aims of this study were to investigate the APP and antioxidant responses in feline malignant mammary tumours, to evaluate their relation with tumour features, and to assess their prognostic value. Serum amyloid A (SAA), haptoglobin (Hp), albumin, butyrylcholinesterase (BChE), insulin‐like growth factor1 (IGF1), paraoxonase1 (PON1), total serum thiols (Thiol), glutathione peroxidase (GPox) and total antioxidant capacity determined by different assays, including trolox equivalent antioxidant capacity assessed by two different methodologies (TEAC1/2), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC), were determined in serum of 50 queens with spontaneous mammary carcinomas and of 12 healthy female cats. At diagnosis, diseased queens presented significantly higher SAA and Hp, and lower albumin, BChE, GPox, TEAC1, TEAC2 and CUPRAC than controls. Different tumour features influenced concentrations of APP and antioxidants. Increases in serum Hp, and decreases in albumin, Thiol and FRAP were significantly associated with neoplastic vascular emboli, metastasis in regional lymph nodes and/or in distant organs. Distant metastasis development during the course of the disease was associated with increases in SAA and TEAC1. At diagnosis, decreased albumin was associated with a longer survival, and BChE <1.15 μmoL/mL.minute was associated with a shorter survival time on multivariate analysis. Feline malignant mammary tumours are associated with an APP response and oxidative stress, and different tumour features influence the inflammatory response and the oxidative damage. Furthermore, some of these analytes proved to have prognostic value.

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“…Therefore, the development of novel adjuvant therapies is crucial [14]. P-cadherin has been recently considered as a potential therapeutic target in human breast cancer [37], and considering the large number of P-cadherin positive feline mammary tumours [16,38] this molecule may constitute a potential therapeutic target in this species too. Therefore, an in vivo model that expresses P-cadherin is fundamental to study the potential anti-Pcadherin therapy in feline mammary tumours.…”

Section: Discussionmentioning

confidence: 99%

An in vitro and in vivo characterization of the cadherin-catenin adhesion complex in a feline mammary carcinoma cell line

Figueira¹,

Gomes²,

Mendes³

et al. 2016

Clin Diagn Pathol

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Feline mammary cancers have high recurrence and metastatic potential. Abnormal expression or function in the major molecules of the cadherin-catenin adhesion complex have been related to breast cancer development and associated to cell migration, invasion and metastatic dissemination. In feline mammary tumours, cadherins and catenins´ role is still poorly known. Therefore, we seek for suitable in vitro and in vivo model systems to study the leading role of P-cadherin and the molecules of the cadherin-catenin complex (CCC) in feline mammary carcinogenesis.Major molecules from the CCC (E-and P-cadherin, α-, β-and p120-catenin) were evaluated in a feline mammary carcinoma cell line (FMCm), by Western blot analysis, immunofluorescence, imunoprecipitation and in situ proximity ligation assay. The FMCm cell line tumourigenic and metastatic capacity was assessed by orthotopically inoculation of a cell suspension in the mammary fat pad of athymic nude mice (N:NIH (S) II-nu/nu). Mice xenografts, as well as metastatic lesions, were evaluated immunohistochemically for cadherins and catenins expression. P-and E-cadherin double-labelling immunofluorescence was also assessed.The FMCm cell line expressed E-and P-cadherin as well as α-, β-and p120-catenin. E-cadherin was showed to interact with each catenin, revealing a putative complete CCC. The cells had E-and P-cadherin co-expression and a close proximity between these two molecules. The FMCm cell line revealed to be highly tumourigenic and metastatic leading to the formation of primary and metastatic lesions in all animals that express all the molecules from the CCC studied. FMCm cell line represents a useful model for in vitro and in vivo studies of feline mammary carcinoma progression.

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Aberrant P-cadherin expression is associated to aggressive feline mammary carcinomas

Cited by 7 publications

References 72 publications

Cat Mammary Tumors: Genetic Models for the Human Counterpart

Cat Mammary Tumors: Genetic Models for the Human Counterpart

Acute phase proteins and biomarkers of oxidative status in feline spontaneous malignant mammary tumours

An in vitro and in vivo characterization of the cadherin-catenin adhesion complex in a feline mammary carcinoma cell line

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